Bioluminescence And Chemiluminescence

The critically acclaimed laboratory standard, Methods in Enzymology, is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. The series contains much material still relevant today - truly an essential publication for researchers in all fields of life sciences.

Bioluminescence and chemiluminescence are among the most important technologies in the life sciences. This latest volume of the long-running biannual Bioluminescence and Chemiluminescence symposium series presents the latest developments in the fundamental and applied aspects of bioluminescence and chemiluminescence. The book covers the fundamental aspects of bioluminescence, including beetle, marine bacterial and Cypridina bioluminescence, and the fundamental aspects of chemiluminescence, including 1,2-dioxetanes. It also presents recent developments in instrumentation and devices and a wide range of applications of bioluminescence and chemiluminescence. The applications are succinctly described and include applications of luminescence in antioxidant research, phagocytosis, microbiology, ecology, food and environmental testing, immunoassay, enzyme assays, DNA probe assays, and reporter gene and gene expression assays. The proceedings have been selected for coverage in: • Biochemistry & Biophysics Citation Index™ • Chemistry Citation Index™ • Index to Scientific & Technical Proceedings® (ISTP® / ISI Proceedings) • Index to Scientific & Technical Proceedings (ISTP CDROM version / ISI Proceedings) • CC Proceedings — Engineering & Physical Sciences • CC Proceedings — Biomedical, Biological & Agricultural Sciences Contents:Bioluminescence and Mating Behavior in Pony Fish, Leiognathus nuchalis (N Azuma et al.)Importance of Firefly Luciferase C-terminal Domain in Binding of Luciferyl-Adenylate (K Ayabe et al.)Effect of Oxygen and Hydrogen Ion on the Modulation of the Bioluminescence from Luminous Bacteria (H Karatani et al.)Superoxide or Singlet Oxygen: The Chemiluminescence of Cypridina Luciferin Analogues in Photodynamic Solutions (M Bancírová & I Šnyrychová)On the Role of Singlet-Oxygen Dimol Chemiluminescence in Dioxirane Reactions (W Adam et al.)On the CIEEL Mechanism of Triggerable Dioxetanes: Does the Electron Jump or Is It Charge Transfer? (W Adam & A V Trofimov)Single-Molecule Imaging of Protein in Living Cells by Pin-Fiber Video-Microscopy (Y Hirakawa et al.)Construction of a Novel Bioluminescence Bacterial Biosensor for Real-Time Monitoring of Cytotoxic Drugs Activity (H M Alloush et al.)The Chemiluminescent Measurement of the Black and Green Tea Antioxidant Capacity and the Comparison with Their Antimicrobial Activity (M Bancírová & I Šnyrychová)Use of Bioluminescent Salmonella typhimurium DT104 to Monitor Uptake and Intracellular Survival Within a Human Cell-Line (J E Angell et al.)Tandem Bioluminescent Enzyme Immunoassay for BDNF and NT-4/5 (S Akahane et al.)Use of the Peroxyoxalate Chemiluminescent Reaction in Acetone in the Presence of Nile Red for the Analysis of Glucose (P Castro-Hartmann et al.)A New Assay for Determining Pyrophosphate Using Pyruvate Phosphate Dikinase and Its Application to DNA Analysis (H Arakawa et al.)and other papers Readership: Scientists in basic luminescene research, analytical chemists and biochemists. Keywords:Chemiluminescene;Bioluminescence;Luciferase;Luciferin;ATP;Bioanalysis;Green Fluroscent Protein (GFP);Imaging;Clinical AnalysisKey Features:Up-to-date coverage of the latest developments in bioluminescence and chemiluminescenceComprehensive coverage of fundamental and applied aspects of bioluminescence and chemiluminescenceLatest experimental procedures and protocols in bioluminescence and chemiluminescence

This complete and well-organized overview of chemiluminescence and bioluminescence is divided into two parts. The first covers historical developments and the fundamental principles of these phenomena before going on to review recent advances and instrumentation. The second part deals with the applications in a variety of research fields including life sciences, drug discovery, diagnostics, environment, agrofood, and forensics. The book is suitable not only for researchers currently employing detection techniques in their research activity, but also for those approaching the subject for the first time. Particular emphasis is placed on the use of chemiluminescence and bioluminescence for the development of a variety of (bio)analytical methods, such as flow-assisted methods, enzyme-, antibody-or gene probe-based assays also in multiplexed formats, miniaturized analytical devices, biosensors, BRET and protein complementation assays, whole-cell biosensors, and bioluminescence molecular imaging. Individual chapters are devoted to the most important and rapidly developing fields including: Instrumentation for Chemiluminescence and Bioluminescence; In vivo, Molecular Imaging; Biotechnological Improvements of Bioluminescent Systems; Cell-based Bioluminescent Biosensors, and Miniaturized Analytical Devices Based on Chemiluminescence, Bioluminescence and Electrochemiluminescence. The book also includes a comprehensive collection of recent bibliographic references.

Since the publication of Bioluminescence and Chemiluminescence, Part B, genes have been cloned that encode luciferases from an array of bioluminescent organisms, novel applications of these genes have been developed, and much has been learned of the fundamental chemistry, biochemistry, structural biology, and biophysics of these intriguing enzymes. New strategies for application of chemiluminescence technology have been developed and refined, promising to further reduce the need to use radioisotopes in basic research and clinical laboratory settings. Methods for detection of low levels of light continue to push the limits of detection, allowing ready monitoring in real time of intricate subcellular processes within living cells. This book affords a glimpse of the state of the art of a rapidly advancing field, and presents to users of these methods a detailed reference of current activities in the field. The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences. Outlines the use of luminescent reporter technologies to monitor gene expression and protein trafficking Describes the luminescence-based clinical assay technologies Details the basic biochemistry, biophysics, and chemistry of light-emitting reactions that are critical for applications Includes explanations of the instrumentation used for detection and quantification of low level light Shows the new applications of luminescence-based technologies that result largely from broad advances in recombinant DNA technologies and nonscale methods

This volume contains the papers presented at the 8th International Symposium on Bioluminescence and Chemiluminescence held at the University of Cambridge in September 1994. These Proceedings provide a substantial account of bioluminescence and chemiluminescence worldwide. The papers are presented in a way that will enable them to be used as a primary source of the most significant research in the area. Papers are grouped into the following areas: chemiluminescence, luminescence as a signal, luminescence in the environment, luminescence in education, methods of ATP and firefly luciferase analyses, molecular biology of luminescence, and imaging of luminescence.

This book focuses on instrumentation of chemi- and bioluminescence and discusses the nature of chemiluminescence as the exothermic oxidation of a substrate organic compound to give an energy-rich product that is luminescent. It describes the applications of chemiluminescence.