Molecular Diagnostics In Cytopathology

Molecular diagnostics are increasingly used to help guide targeted therapy in solid organ tumors and hematologic malignancies. A large proportion of molecular testing is performed on limited-volume samples obtained via minimally invasive techniques, such as fine needle aspiration. Increasingly, cytopathologists play an essential role in this process, both in the triage of specimens during rapid on-site evaluation and in the evaluation of archival samples to determine suitability for ancillary testing. Therefore, it is imperative that practicing cytopathologists stay abreast of up-to-date diagnostic, prognostic, and predictive ancillary tests that can be used on limited cytologic material. This is a challenge since the landscape of known genomic alterations is constantly evolving and the subsequent set of testing options is ever expanding. The proposed text will provide a user-friendly quick-reference handbook to serve as a useful resource for practicing pathologists and laboratory personnel dealing with, and interested in, this evolving field of molecular cytopathology. Essential components to be presented include: 1) pre-analytic factors that affect sample selection and evaluation; 2) specimen preparation to maximize confidence in results; 3) interpretation of results; 4) potential limitations; and 5) workflow algorithms. In addition, specific disease specific molecular testing details will be outlined to provide the reader with resources for quick reference. All chapters will be written by experts in their fields and will include the most up-to-date scientific and clinical information. Molecular Diagnostics in Cytopathology will be of value to Cytopathologists, Cytotechnologists, Cytotechnology students, Cytopathology fellows, Surgical pathologists, Pathology residents and fellows, Molecular Pathologists, Molecular pathology fellows, Molecular technologists, as well as Translational researchers with an interest in molecular cytopathology.

This book reviews the current applications of molecular tools in cytopathology and provides a concise handbook for those who provide care in this era of personalized medicine. Specifically, the text provides a comprehensive and concise review of the emerging molecular tests available clinically in different subspecialities of diagnostic pathology. It reviews the current data of molecular testing already applied in cytopathology, discusses some of the biomarkers with potential utility in cytopathology in the near future and reviews the technical challenges in applying and validating molecular tools in liquid-based cytologic materials. Molecular Cytopathology will serve as a valuable resource for cytopathologists, cytotechnologists, pathology trainees, and clinicians with an interest in molecular applications in cytopathology.

This volume in the Foundations in Diagnostic Pathology Series packs today's most essential cell and tissue base molecular pathology into a compact, high-yield format! It focuses on the state of the art in practical validated molecular diagnostics as applied across the fields of surgical pathology and cytology. With an emphasis on current, clinically valid, and diagnostically important applications today and in the near future, you can be assured you’re getting the most up-to-date, authoritative coverage available. Its pragmatic, well-organized approach, nearly 250 full-color illustrations, and at-a-glance boxes and tables make the information you need easy to access. Practical and affordable, this resource is ideal for study and review as well as everyday clinical practice! Offers detailed discussions on today’s technologies to help you select the best test for case evaluation. Presents recognized molecular pathologists who convey the most current information, keeping you on the cusp of your field. Features nearly 250 full-color illustrations that present important pathologic features, enabling you to form a differential diagnosis and compare your findings with actual cases. Uses a consistent, user-friendly format, including at-a-glance boxes and tables for easy reference.

This text presents comprehensive review of the recommendations of the Papanicolaou Society of Cytopathology for reporting results of respiratory cytology specimens along with advances in diagnostic, prognostic, and predictive immunohistochemical and molecular techniques which can be performed on cytology specimens.The majority of the text focuses on the standardized terminology and nomenclature for respiratory cytology as recommended by the Papanicolaou Society of Cytopathology. Each of the diagnostic categories are described in depth and formal definitions for each category are given along with cytologic criteria. Explanatory notes are given discussing limitations of the category as well as its malignancy risk and reproducibility. Management recommendations are supplied for each category. Sample reports documenting the preferred reporting format are also given. Each category is associated with a reference list establishing the evidence based nature of the categories. An in depth discussion of ancillary testing is provided, including the utilization of microbiologic culture, immunohistochemistry, and molecular techniques. Substantial emphasis is placed on molecular diagnostics necessary for optimization of personalized testing and the appropriate use of targeted therapies. The text represents a comprehensive resource for the state of the science of the cytologic diagnosis of respiratory lesions.

This book is intended for practicing pathologists and cytopathologists, as well as for pathology trainees and cytotechnicians. It starts with a detailed description of the extremely important pre-analytical phase for molecular testing followed by a presentation of the key tests and their application in different organs, e.g. the lung or thyroid. Step-by-step instructions for the different assays, reporting and clinical integration of the test results are discussed. The authors help the reader to benefit from their experiences by providing a valuable tool for the implementation of these techniques in daily practice. Though the use of molecular techniques is well established in surgical biopsies, to date they are not widely used in connection with cytological material. However, in some fields like lung cancer or aspirates from the pancreas and biliary tract the only available material for diagnosis is the cytological preparation a fact that has created a need for the standardization of molecular techniques on cytology.

The Year Book of Pathology and Laboratory Medicine brings you abstracts of the articles that reported the year's breakthrough developments in pathology and laboratory medicine, carefully selected from more than 300 journals worldwide. Expert commentaries evaluate the clinical importance of each article and discuss its application to your practice. There's no faster or easier way to stay informed! Chapters in this annual cover the most current information on all aspects of pathology and laboratory medicine including: molecular diagnostics, dermatopathology, anatomic pathology techniques, outcomes analysis, cytopathology, clinical immunology, clinical microbiology, neuropathology and hematology.

Diagnostic Cytopathology Board Review and Self-Assessment provides a comprehensive systems-based review of non-gynecological cytology including cytomorphology, pitfalls and ancillary studies, presented in a high-yield format with board-type multiple choice questions and detailed answers. It provides an excellent review, resource and self-assessment for pathologists, cytopathologists and cytotechnologists, as well as trainees (pathology residents, cytopathology fellows and cytotechology students) who are preparing for board examinations or in-service examinations, in addition to those who are looking to fine-tune their cytology diagnostic skills. The volume is organized in a systems-based format including chapters covering lung, lymph nodes, pancreas, liver, gastrointestinal tract, thyroid, salivary gland, central nervous system, soft tissue and bone, kidney, adrenal, retroperitoneal and exfoliative cytology of urine, serous fluids and CSF. This book is enriched with the following features to maximize studying and review of cytopathology" HIGH YIELD REVIEW: Concise high-yield review with tables emphasizing key points, useful criteria and important concepts to maximize your score on board examinations and your diagnostic knowledge of exfoliative and aspiration cytopathology. BOARD EXAM-TYPE QUESTIONS: Over 800 board exam-type questions covering all areas of non-gynecological cytology with emphasis on cytomorphology, diagnostic pitfalls and ancillary studies, including detailed answers with in-depth discussions and key references from the literature and major cytopathology text books. HIGH QUALITY FULL-COLOR IMAGES: Over 600 high-quality full-color photomicrographs, including images of immunohistochemical stains and other ancillary studies performed.

Accompanying CD-ROM contains ... "a companion eBook version of Molecular diagnostics : for the clinical laboratorian, Second edition ... for downloading and use in the reader's PC or PDA."--Page 4 of cover.