Plant Microtechniques and Protocols

Plant Microtechniques and Protocols
Author: Edward Chee Tak Yeung,Claudio Stasolla,Michael John Sumner,Bing Quan Huang
Publsiher: Springer
Total Pages: 576
Release: 2015-09-19
Genre: Science
ISBN: 9783319199443

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A proper understanding of the structural organization of the plant body is essential to any study in plant biology. Experimental studies in vivo and in situ will lead to structural, physiological, and cellular changes of the experimental material. To study macroscopic and microscopic changes, different histological methods and microtechniques can be used as they provide valuable information of the experimental system. In addition, the observed structural changes allow investigators to set hypothesis for further studies based on one’s own observation. Thus, proper selection and utilization of microtechniques are a must for the success of a research program. At present, an up-to-date collection of protocols are not readily available in the literature. The latest work in plant microtechniques was published in 1999 by Ruzin but many others are no longer in print [e.g., Jensen (1964); O’Brien and McCully (1981)]. Furthermore, a majority of published works focus on techniques related to general processing and staining procedures. A comprehensive treatment that encompasses broader applications of microtechniques to other disciplines is lacking [e.g., archeology, wood science, etc.]. There is a need to create a comprehensive volume of botanical methods and protocols which includes traditional and novel techniques that can be used by researchers in plant science and investigators in other disciplines that require plant microtechniques in their research and teaching. This book covers a wide variety of applications and brings them up-to-date to make them understandable and relevant, especially to students using the methods for the first time. It is our intention to create a useful reference for plant histology and related methods that will serve as a foundation for plant scholars, researchers, and teachers in the plant sciences.​

Plant Microtechniques and Protocols

Plant Microtechniques and Protocols
Author: Edward Chee Tak Yeung,Claudio Stasolla,Michael John Sumner,Bing Quan Huang
Publsiher: Unknown
Total Pages: 135
Release: 2015
Genre: Electronic Book
ISBN: 3319199455

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This work gathers common plant microtechniques and updates their procedures using a simple and fully understandable approach. The text serves as a handy resource for scientists familiar with the protocols and as a guide for the novice, especially students just beginning to learn about various structural methods for the first time. This book analyzes a range of topics in order to generate cross-talks among scientists in different research disciplines. The first section of this volume covers the more commonly used embedding methods, with emphasis on the preparative methods for light and electron microscopy. A number of cell biology related protocols are compiled in the second section to showcase the usefulness of various techniques based on different processing and staining methods. The third section highlights some common and recent procedures in wood preparation. The last section includes botanical methods related to archaeological uses of plant materials. A special chapter on field and herbarium procedures is also included to serve as a guide to students interested in plant collection and taxonomic studies.

Plant Microtechnique and Microscopy

Plant Microtechnique and Microscopy
Author: Steven E. Ruzin
Publsiher: Oxford University Press, USA
Total Pages: 340
Release: 1999
Genre: Science
ISBN: 0195089561

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This text provides in-depth coverage of the field of microtechnique. The traditional paraffin method is covered in explicit step-by-step protocols, with theoretical background included to give students the tools required to design their own experiments and interpret existing results. In addition, the theory and practice of modern applications, such as methacrylate embedding and sectioning, microwave tissue processing, fluorescence histochemistry, and in-situ hybridization, are documented thoroughly. Preceding the first chapter are ten single-page Quick Start protocols. Patterned after computer software manuals, these abbreviated instructions provide the experienced student with the fundamental steps to complete the ten microtechnique protocols most frequently used today. Appendices on laboratory practice (chemical toxicities, common calculations and buffer tables) are provided.

Plant Tissue Culture New Techniques and Application in Horticultural Species of Tropical Region

Plant Tissue Culture  New Techniques and Application in Horticultural Species of Tropical Region
Author: Duong Tan Nhut,Hoang Thanh Tung,Edward Chee-Tak YEUNG
Publsiher: Springer Nature
Total Pages: 400
Release: 2022-04-05
Genre: Science
ISBN: 9789811664984

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This book presents latest work in the field of plant biotechnology regarding high-efficiency micropropagation for commercial exploitation at low labor and equipment costs. The book consists of 18 chapters on establishing advanced culture systems, techniques as well as latest modification protocols on a variety of crops. It also discusses new methods such as nylon film culture system, light-emitting diode and wireless light-emitting diode system, stem elongation, wounding manipulation and shoot tip removal, in vitro hydroponic and microponic culture system, thin cell layer culture system etc. Plant cell tissue has been developed more than fifty years ago. Since then applications of in vitro plant propagation expanded rapidly all around the world and played as an important role in agricultural and horticultural systems. This book will be of interest to teachers, researchers, scientists, capacity builders and policymakers. Also the book serves as additional reading material for undergraduate and graduate students of agriculture, forestry, ecology, soil science, and environmental sciences.

Protocols for Macroalgae Research

Protocols for Macroalgae Research
Author: Bénédicte Charrier,Thomas Wichard,C R K Reddy
Publsiher: CRC Press
Total Pages: 421
Release: 2018-04-17
Genre: Medical
ISBN: 9781351132930

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This book presents a wide range of tested and proven protocols relevant to a number of fields within biotechnology used in laboratory experiments in everyday phycological (seaweed) research. A major focus will be on bioenergy related aspects of this emerging technology. These protocols will be written in a clear and concise manner using simple language permitting even nonspecialist to adequately understand the significance of this research. It will also contain all necesssary notes and guidelines for successful execution of these experiments.

Plant Cell Biology

Plant Cell Biology
Author: Anonim
Publsiher: Academic Press
Total Pages: 456
Release: 2020-08-31
Genre: Science
ISBN: 9780128215340

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Plant Cell Biology, volume 160 in "Methods in Cell Biology", includes chapters on modern experimental procedures and applications developed for research in the broad area of plant cell biology. Topics covered in this volume include techniques for imaging and analyzing membrane dynamics and movement across membranes; cell wall composition, structure and mechanics; cytoskeleton dynamics and organization; cell development; ion channel physiology; cell mechanics; and methods related to quantifying cell morphogenesis. Provide in-depth procedures and application notes from selected experts who developed the methods Each chapter will include figures and movies as appropriate to explain complex techniques Chapters will include caveats of techniques and future prospects

Plant Microtechnique and Microscopy

Plant Microtechnique and Microscopy
Author: Steven E. Ruzin
Publsiher: Oxford University Press, USA
Total Pages: 322
Release: 1999
Genre: Science
ISBN: 0195089561

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Plant microtechnique has generated renewed interest in recent years, due in part to the need for molecular biologists to visualize a gene or gene product in the context of the whole plant. Plant Microtechnique and Microscopy offers uniquely in-depth coverage of this reinvigorated field. Thoroughly covering classical aspects of microscope slide preparation, it goes a step beyond all other available manuals by also documenting the theory and practice of modern applications. The text opens with single-page "Quick Start" protocols that provide students with fundamental instructions to complete eight of the most common microtechnique protocols used today. The following sections cover the theory and practice of microtechnique. The traditional paraffin method is demonstrated by explicit step-by-step protocols, and theoretical background is incorporated to give students the tools required to design their own experiments and to interpret existing results. In addition, modern applications such as methacrylate embedding and sectioning, microwave tissue processing, fluorescence histochemistry, and in situ hybridization are discussed in detail. The manual also contains a definitive chapter on microscopy and describes, in both text and diagrams, the optical principles of techniques such as phase contrast and DIC as well as confocal and deconvolution wide-field microscopy. Appendices on laboratory practice (chemical toxicities, common calculations, and buffer tables), an extensive appendix on optics and its application to the microscope, and an extensive bibliography of over 550 references are also included. Ideal for courses in plant biology, Plant Microtechnique and Microscopy also serves as an indispensable reference for all students of microscopy, histology, and histological technique. It is a valuable addition to every biological laboratory.

Plant Pathogen Interactions

Plant Pathogen Interactions
Author: Nora A. Foroud,Jonathan A. D. Neilson
Publsiher: Springer Nature
Total Pages: 242
Release: 2023-05-30
Genre: Science
ISBN: 9781071631591

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This detailed collection focuses on current methodologies for studying various crop diseases. The book explores phytopathogen detection, host-pathogen interactions at the earliest stages of colonization, RNA-seq analyses, as well as host responses downstream of, or coincidental to, gene expression and a newly established protocol for gene editing of Medicago sativa. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Plant-Pathogen Interactions serves as an ideal guide for researchers working in the vital area of plant diseases.