Single Molecule Cellular Biophysics
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Single Molecule Cellular Biophysics
Author | : Mark C. Leake |
Publsiher | : Cambridge University Press |
Total Pages | : 135 |
Release | : 2013-01-31 |
Genre | : Science |
ISBN | : 9781139619349 |
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Recent advances in single molecule science have presented a new branch of science: single molecule cellular biophysics, combining classical cell biology with cutting-edge single molecule biophysics. This textbook explains the essential elements of this new discipline, from the state-of-the-art single molecule techniques to real-world applications in unravelling the inner workings of the cell. Every effort has been made to ensure the text can be easily understood by students from both the physical and life sciences. Mathematical derivations are kept to a minimum whilst unnecessary biological terminology is avoided and text boxes provide readers from either background with additional information. 100 end-of-chapter exercises are divided into those aimed at physical sciences students, those aimed at life science students and those that can be tackled by students from both disciplines. The use of case studies and real research examples make this textbook indispensable for undergraduate students entering this exciting field.
Single Molecule Cellular Biophysics
Author | : Royal Society (Great Britain) |
Publsiher | : Unknown |
Total Pages | : 135 |
Release | : 2012 |
Genre | : Cytology |
ISBN | : 0854039902 |
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Handbook of Single Molecule Biophysics
Author | : Peter Hinterdorfer,Antoine van Oijen |
Publsiher | : Springer Science & Business Media |
Total Pages | : 634 |
Release | : 2009-12-24 |
Genre | : Science |
ISBN | : 9780387764979 |
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This handbook describes experimental techniques to monitor and manipulate individual biomolecules, including fluorescence detection, atomic force microscopy, and optical and magnetic trapping. It includes single-molecule studies of physical properties of biomolecules such as folding, polymer physics of protein and DNA, enzymology and biochemistry, single molecules in the membrane, and single-molecule techniques in living cells.
An Introduction to Single Molecule Biophysics
Author | : Yuri L. Lyubchenko |
Publsiher | : CRC Press |
Total Pages | : 240 |
Release | : 2017-11-22 |
Genre | : Science |
ISBN | : 9781351333122 |
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This book gives an accessible, detailed overview on techniques of single molecule biophysics (SMB), showing how they are applied to numerous biological problems associated with understanding the molecular mechanisms of DNA replication, transcription, and translation, as well as functioning of molecular machines. It covers major single molecule imaging and probing techniques, highlighting key strengths and limitations of each method using recent examples. The chapters begin with a discussion of single molecule fluorescence techniques followed by an overview of the atomic force microscope and its use for direct time-lapse visualization of dynamics of molecular complexes at the nanoscale, as well as applications in measurements of interactions between molecules and mechanical properties of isolated molecules and their complexes. The next chapters address magnetic tweezers and optical tweezers, including instrumentation, fundamentals of operation, and applications. A final chapter turns to nanopore transport and nanopore-based DNA sequencing technology that will play a major role in next-generation genomics and healthcare applications.
Single Molecule Biophysics
Author | : Tamiki Komatsuzaki,Masaru Kawakami,Satoshi Takahashi,Haw Yang,Robert J. Silbey |
Publsiher | : John Wiley & Sons |
Total Pages | : 525 |
Release | : 2011-11-16 |
Genre | : Science |
ISBN | : 9781118131381 |
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Discover the experimental and theoretical developments in optical single-molecule spectroscopy that are changing the ways we think about molecules and atoms The Advances in Chemical Physics series provides the chemical physics field with a forum for critical, authoritative evaluations of advances in every area of the discipline. This latest volume explores the advent of optical single-molecule spectroscopy, and how atomic force microscopy has empowered novel experiments on individual biomolecules, opening up new frontiers in molecular and cell biology and leading to new theoretical approaches and insights. Organized into two parts—one experimental, the other theoretical—this volume explores advances across the field of single-molecule biophysics, presenting new perspectives on the theoretical properties of atoms and molecules. Single-molecule experiments have provided fresh perspectives on questions such as how proteins fold to specific conformations from highly heterogeneous structures, how signal transductions take place on the molecular level, and how proteins behave in membranes and living cells.This volume is designed to further contribute to the rapid development of single-molecule biophysics research. Filled with cutting-edge research reported in a cohesive manner not found elsewhere in the literature, each volume of the Advances in Chemical Physics series serves as the perfect supplement to any advanced graduate class devoted to the study of chemical physics.
Molecular and Cellular Biophysics
Author | : Meyer B. Jackson |
Publsiher | : Cambridge University Press |
Total Pages | : 135 |
Release | : 2006-01-12 |
Genre | : Science |
ISBN | : 1139447246 |
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Molecular and Cellular Biophysics provides advanced undergraduate and graduate students with a foundation in the basic concepts of biophysics. Students who have taken physical chemistry and calculus courses will find this book an accessible and valuable aid in learning how these concepts can be used in biological research. The text provides a rigorous treatment of the fundamental theories in biophysics and illustrates their application with examples. Conformational transitions of proteins are studied first using thermodynamics, and subsequently with kinetics. Allosteric theory is developed as the synthesis of conformational transitions and association reactions. Basic ideas of thermodynamics and kinetics are applied to topics such as protein folding, enzyme catalysis and ion channel permeation. These concepts are then used as the building blocks in a treatment of membrane excitability. Through these examples, students will gain an understanding of the general importance and broad applicability of biophysical principles to biological problems.
Single Molecule Biophysics and Poisson Process Approach to Statistical Mechanics
Author | : Susanta K Sarkar |
Publsiher | : Morgan & Claypool Publishers |
Total Pages | : 74 |
Release | : 2016-04-01 |
Genre | : Science |
ISBN | : 9781681741161 |
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This is an overview of single molecule physics, the study of both equilibrium and non-equilibrium properties at the single molecule level. It begins with an introduction to this fascinating science and includes a chapter on how to build the most popular instrument for single molecule biophysics, the total internal reflection fluorescence (TIRF) microscope. It concludes with the Poisson process approach to statistical mechanics, explaining how to relate the process to diverse areas and see how data analysis and error bars are integral parts of science.
Single Molecules and Nanotechnology
Author | : Rudolf Rigler,H. Vogel |
Publsiher | : Springer Science & Business Media |
Total Pages | : 318 |
Release | : 2007-12-07 |
Genre | : Technology & Engineering |
ISBN | : 9783540739241 |
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This book focuses on recent advances in the rapidly evolving field of single molecule research. These advances are of importance for the investigation of biopolymers and cellular biochemical reactions, and are essential to the development of quantitative biology. Written by leading experts in the field, the articles cover a broad range of topics, including quantum photonics of organic dyes and inorganic nanoparticles and monitoring of single molecule (enzymatic) reactions.