Bacillus Thuringiensis Biotechnology

Bacillus thuringiensis (Bt) has been used as a biopesticide in agriculture, forestry and mosquito control because of its advantages of specific toxicity against target insects, lack of polluting residues and safety to non-target organisms. The insecticidal properties of this bacterium are due to insecticidal proteins produced during sporulation. Despite these ecological benefits, the use of Bt biopesticides has lagged behind the synthetic chemicals. Genetic improvement of Bt natural strains, in particular Bt recombination, offers a promising means of improving efficacy and cost-effectiveness of Bt-based bioinsecticide products to develop new biotechnological applications. On the other hand, the different Bacillus species have important biotechnological applications; one of them is carried out by producing secondary metabolites, which are the study object of natural product chemistry. The amazing structural variability of these compounds has attracted the curiosity of chemists and the biological activities possessed by natural products have inspired the pharmaceutical industry to search for lead structures in microbial extracts. Screening of microbial extracts reveals the large structural diversity of natural compounds with broad biological activities, such as antimicrobial, antiviral, immunosuppressive, and antitumor activities that enable the bacterium to survive in its natural environment. These findings widen the target range of Bacillus spp., in special B. thuringiensis, besides insecticidal activity and help people to better understand its role in soil ecosystem.

This book describes new strategies being used to combat disease agents and invertebrate pests. Outstanding experts from the United States, Belgium, China, Guatemala, Japan, Philippines, Singapore, and Thailand have contributed chapters that cover the latest achievements in genetic engineering, emphasizing the microbial and viral biological control agents that can provide environmentally safe, economical control systems. Topics discussed include genetic engineering of Bacillus thuringiensis and B. sphaericus, the development of insect resistance to microbial biocontrol agents, engineering of baculoviruses and nematodes, bioengineering of plants, plant transformation by particle bombardment, fusion of cultured insect cells, new immunodiagnostic assays and control measures against parasitic human diseases, and genetically engineered microbial agents for malaria control. The book also presents improved mass production procedures of microbial and viral biocontrol agents, as well as regulatory and environmental aspects of genetically engineered biocontrol agents. Biotechnology for Biological Control of Pests and Vectors will provide a valuable reference for researchers and students of biological control, microbiology, virology, and molecular biology.

This textbook introduces to the fundamentals and applications of insect biotechnology in a concise manner. Global economically important insects such as silkworm, lac or honey bee are in the focus of this text. The book attempts to address all of the latest developments in this growing field such as application of metagenomics and proteomics in mining for genes and enzymes that could be beneficial in diverse industrial and biomedical applications. Further, insects have been established as suitable tool for the production of transgenic products which is discussed in one dedicated chapter. The book targets students and researchers in molecular biology, biotechnology or entomology who want to get familiar with the emerging field of insect biotechnology.

Probing the profitable new science of creating--and altering--life forms "Extraordinarily well documented . . . remarkably clear. This is the most comprehensive coverage of these issues to date. It will be required reading for some time." -- Lawrence Busch, Michigan State University "Krimsky and Wrubel not only describe the components of agricultural biotechnology, they address and analyze controversies involving the risks and benefits of new technologies. Coverage of technical to social components of agricultural biotechnology is unusually complete and thorough. Their even-handed and comprehensive approach to these topics is rare and extremely valuable." -- Richard Weinzierl, University of Illinois Modern agriculture is being transformed by the genetic alteration of seeds, animals, and microorganisms, a process that has produced such products as flavor saver tomatoes and crops resistant to specific insects or herbicides. Agricultural Biotechnology and the Environment is the first comprehensive overview of the ongoing transformation of agriculture, exploring the impact of genetic engineering from scientific, social, ethical, and ecological perspectives. Sheldon Krimsky and Roger Wrubel detail the impact the new generation of products is expected to have on agricultural practice and the environment and assess the degree to which current trends in biotechnology match earlier expectations. They also analyze the social and political response to innovations resulting from genetic technology. Closely examined in each of three areas--transgenic plants, genetically engineered microorganisms, and transgenic animals--are technical and scientific problems, social controversies, and anticipated environmental impacts. An objective, detailed look at a subject of interest to a broad spectrum of readers, Agricultural Biotechnology and the Environment will be of interest to researchers in the new biotechnology fields as well as to educated general readers and policymakers. A volume in the series The Environment and the Human Condition

Transgenic crops are now grown in the USA and several other countries, and are now entering some of the developing countries. It was felt that few people in most of the Central American countries knew about the potential benefits and risks of the new technology of transgenic crops, so a workshop was held at the Panamerican Agriculture College in Honduras in May 1996. This publication sets out the presentations, discussion and recommendations of this regional workshop, with reference to cotton, maize and rice crops, legislation aspects and pest resistance management. The booklet with 11 chapters provides an interesting overview of the subject, which would be of interest to other areas of the world. (Review by G.A. Matthews, in: Crop Protection vol. 6, issue 5 (1997)p. 492)

Due to increasing problems occurring from massive applications of pesticides, such as insect resistance to pesticides, the use of biotechnological tools to minimize losses from insect pests has become inevitable. Presenting alternative strategies for alleviating biotic stresses, Biotechnological Approaches for Pest Management and Ecological Sustain