Basic And Advanced Laboratory Techniques In Histopathology And Cytology

The second edition of this well-received book provides detailed information on the basic and advanced laboratory techniques in histopathology and cytology. It offers clear guidance on the principles and techniques of routine and special laboratory techniques. It also covers advanced laboratory techniques such as immunocytochemistry, flow cytometry, liquid-based cytology, polymerase chain reactions, tissue microarray, molecular technology, etc. The book's second edition covers several important recent topics with many new chapters, such as liquid biopsy, artificial neural network, digital pathology, and next-generation sequencing. Each chapter elucidates basic principle, practical methods, troubleshooting, and clinical applications of the technique. It includes multiple colored line drawings, microphotographs, and tables to illustrate each technique. The book is a helpful guide to the post-graduate students and fellows in pathology, practicing pathologists, as well as laboratory technicians, and research students.

This is a brand new edition of the leading reference work on histological techniques. It is an essential and invaluable resource suited to all those involved with histological preparations and applications, from the student to the highly experienced laboratory professional. This is a one stop reference book that the trainee histotechnologist can purchase at the beginning of his career and which will remain valuable to him as he increasingly gains experience in daily practice. Thoroughly revised and up-dated edition of the standard reference work in histotechnology that successfully integrates both theory and practice.Provides a single comprehensive resource on the tried and tested investigative techniques as well as coverage of the latest technical developments. Over 30 international expert contributors all of whom are involved in teaching, research and practice.Provides authoritative guidance on principles and practice of fixation and staining. Extensive use of summary tables, charts and boxes.Information is well set out and easy to retrieve. Six useful appendices included (SI units, solution preparation, specimen mounting, solubility). Provides practical information on measurements, preparation solutions that are used in daily laboratory practice. Color photomicrographs used extensively throughout. Better replicates the actual appearance of the specimen under the microscope. Brand new co-editors. New material on immunohistochemical and molecular diagnostic techniques.Enables user to keep abreast of latest advances in the field.

This book is a practical guide to histopathological and cytopathological techniques for disease detection and diagnosis. Divided into fifteen chapter, the text begins with an overview of cells and tissue, discussion on microscopy, and an introduction to the importance of histopathology. The following sections cover different techniques, each describing basic theory, procedure, potential difficulties, and then concluding with important subjective and objective questions. Recent developments in the field including immunochemistry, automation, and microarray, are also discussed. Each technique is explained with the help of diagrams and figures to assist understanding. Key points Practical guide to histopathological and cytopathological techniques Presented in a step by step approach, with illustrative diagrams and figures Discusses recent advances and procedures Includes chapter on safety in the histopathology laboratory

In the era of precision medicine, physicians are increasingly in need of more definitive diagnostic, prognostic, and predictive information derived from small biopsy specimens such as cytology samples in order to guide effective patient care. Cytopathology is well poised to meet this challenge. Whilst the traditional cytomorphologic component of cytology practice is still valid, enormous advances have been made in the field of cytopathology thanks to transformative technology and innovative individuals that have augmented the cytologists' ability to meet the demands of modern medicine. The purpose of this book is to describe, illustrate, and review many of the most recent developments regarding modern techniques employed in cytopathology. This latest monograph is intended for all cytologists including cytopathologists, cytotechnologists, cytology lab assistants, trainees, research scientists, and anyone who is interested in the field of cytopathology. We have invited pioneering experts in their respective fields to author these chapters. This book is not only the culmination of their groundbreaking work and effort but also presents a critical review of the current literature. We have attempted to provide readers with an informative and comprehensive aid so that they may better appreciate how emerging technology has been applied to cytology. Each chapter in this book presents a stand-alone contemporary review of emerging topics in cytopathology. We hope that you will find this monograph thought-provoking and a valuable reference for your practice.

This new, full-color resource is the only book to document the full range of normal cytologic findings encountered in all body areas. It covers both exfoliative and fine- needle aspiration cytology with an emphasis on standard (Papanicolaou and Diff-Quik) stains. Over 1,140 outstanding full-color images vividly display the complete spectrum of normal samples at a variety of magnifications.

This book is a complete guide to histopathology techniques for trainees. Beginning with an introduction to tissue examination, the next chapters discuss fixation and fixatives, tissue processing and embedding, decalcification, microtomy and section cutting, and frozen section and cryostat. The following sections cover different staining procedures, immunohistochemistry, and automation in histopathology, concluding with chapters on biological waste management and quality management. Each chapter includes a self-assessment exercise with short notes and answers, and the comprehensive text is further enhanced by nearly 350 clinical photographs, diagrams and tables. Key points Complete guide to histopathology techniques for trainees Provides detail on different staining procedures, immunohistochemistry, and automation Features self-assessment exercises with notes and answers Highly illustrated with clinical photographs, diagrams and tables

Covering anatomical, clinical, pathological and laboratory aspects of surgical histopathology specimens, Histopathology Specimens: Clinical, Pathological and Laboratory Aspects, Second Edition relates specimen dissection and its clinical context to relevant histopathology reports, and therefore a more comprehensive patient prognosis and management is possible. Histopathology Specimens: Clinical, Pathological and Laboratory Aspects, Second Edition explains pathological and clinical terminology, including a glossary of clinical request form abbreviations. A standardised step-wise approach to specimen handling is illustrated with simple line diagrams and highlights essentials of the histopathology report, relating them to appropriate specimen dissection. The integrated multidisciplinary team approach taken to the modern clinical management of patients is reflected by correlating patient presentation, diagnostic and staging investigations with histopathology specimens. Current WHO and TNM tumor classifications are referenced. Histopathology Specimens: Clinical, Pathological and Laboratory Aspects, Second Edition will be of educative value and act as a reference tool for the medical undergraduate student, medical trainee in histopathology and the biomedical scientist, and as a useful aide memoire for the histopathology consultant.

Master the art and science of specimen collection, preparation, and evaluation with Canine & Feline Cytology: A Color Atlas and Interpretation Guide, Second Edition. This easy-to-use guide covers all body systems and fluids including a special chapter on acquisition and management of cytology specimens. Hundreds of vivid color images of normal tissue alongside abnormal tissue images - plus concise summaries of individual lesions and guidelines for interpretation - will enhance your ability to confidently face any diagnostic challenge. A greatly expanded image collection, with more than 1,200 vivid, full-color photomicrographic illustrations depicting multiple variations of normal and abnormal tissue for fast and accurate diagnosis Clear, concise descriptions of tissue sampling techniques, slide preparation and examination guidelines Helpful hints for avoiding technical pitfalls and improving diagnostic quality of specimens Includes all body systems and fluids as well as pathological changes associated with infectious agents Histologic and histopathologic correlates provided in all organ system chapters. User-friendly format and logical organization facilitates readability and learning. Expert contributors represent the most respected leaders in the field. NEW! Chapter on Fecal Cytology Highlighted boxes featuring Key Points provide helpful tips for best conceptual understanding and diagnostic effectiveness Photomicrographs now include more comparative histology Discussions of broader uses of stains and immunocytochemistry for differential cytologic characterization Expanded chapter on Advanced Diagnostic Techniques includes more methodology and application of current tools, representing advances in both aspiration and exfoliative cytology.