Biochemical And Immuno Histochemical Analysis Of App Processing And Amyloid Pathology In Single And Multiple Transgenic Mice As Models For Alzheimer S Disease

In Alzheimer's Disease (AD), the Amyloid Precursor Protein (APP) is endoproteolytically cleaved by beta-secretase to liberate beta-stub and subsequently processed by beta-secretase to produce Amyloid-beta (AP). Considering these endoproteolytic products have been implicated in AD pathogenesis, we have modified APP such that the cytoplasmic domain is absent and unable to support full-length beta-stub synthesis, yet able to produce full-length Abeta. By engineering mice with this transgene, we can assess whether Abeta or beta-stub cause cognitive deficits as compared to TgCRND8 mice that support synthesis of full length APP, beta-stub and Abeta. Moreover, transgenes with an altered APP copper binding domain (CuBD) have been made to prevent the post-natal lethality seen in TgCRND8 mice, while still exhibiting AD pathology. Through genetic, biochemical, and behavioural analyses of our transgenic mouse models, we will be able to define the contributions of the cytoplasmic tail and the CuBD of APP in AD pathogenesis.