Biotrophic Plant Microbe Interactions

Throughout their life, plants interact with all sorts of microbes. Some of these are detrimental and cause disease; some interactions are mutually beneficial for both partners. It is clear that most, if not all, of the interactions are regulated by highly complex checks and balances sustained by signalling and exchange of messengers and nutrients. The interactions where both partners are alive for a significant part of their time together are called biotrophic. In this e-book we bring together 33 articles representing the current state-of-the-art in research about diverse biotrophic plant-microbe associations aimed at describing and understanding how these complex and ubiquitous partnerships work and ultimately support much of the land-based biosphere.

Throughout their life, plants interact with all sorts of microbes. Some of these are detrimental and cause disease; some interactions are mutually beneficial for both partners. It is clear that most, if not all, of the interactions are regulated by highly complex checks and balances sustained by signalling and exchange of messengers and nutrients. The interactions where both partners are alive for a significant part of their time together are called biotrophic. In this e-book we bring together 33 articles representing the current state-of-the-art in research about diverse biotrophic plant-microbe associations aimed at describing and understanding how these complex and ubiquitous partnerships work and ultimately support much of the land-based biosphere.

Plants and microbes interact in a complex relationship that can have both harmful and beneficial impacts on both plant and microbial communities. Effectors, secreted microbial molecules that alter plant processes and facilitate colonization, are central to understanding the complicated interplay between plants and microbes. Effectors in Plant-Microbe Interactions unlocks the molecular basis of this important class of microbial molecules and describes their diverse and complex interactions with host plants. Effectors in Plant Microbe Interactions is divided into five sections that take stock of the current knowledge on effectors of plant-associated organisms. Coverage ranges from the impact of bacterial, fungal and oomycete effectors on plant immunity and high-throughput genomic analysis of effectors to the function and trafficking of these microbial molecules. The final section looks at effectors secreted by other eukaryotic microbes that are the focus of current and future research efforts. Written by leading international experts in plant-microbe interactions, Effectors in Plant Microbe Interactions, will be an essential volume for plant biologists, microbiologists, pathologists, and geneticists.

This book, divided into 13 chapters, explores recent discoveries in the area of molecular plant-microbe interactions. It focuses mainly on the mechanisms controlling plant disease resistance and the cross talk among the signalling pathways involved, and the strategies used by fungi and viruses to suppress these defences. Two chapters deal with the role of symbionts (such as the symbiotic actinobacteria and vesicular arbuscular mycorrhizal fungi) during their interactions with plants.

Secretomics describes the global study of proteins that are secreted by a cell, a tissue or an organism, and has recently emerged as a field for which interest is rapidly growing. The term secretome was first coined at the turn of the millennium and was defined to comprise not only the native secreted proteins released into the extracellular space but also the components of machineries for protein secretion. Two secretory pathways have been described in fungi: i) the canonical pathway through which proteins bearing a N-terminal peptide signal can traverse the endoplasmic reticulum and Golgi apparatus, and ii) the unconventional pathway for proteins lacking a peptide signal. Protein secretion systems are more diverse in bacteria, in which types I to VII pathways as well as Sec or two-arginine (Tat) pathways have been described. In oomycete species, effectors are mostly small proteins containing an N-terminal signal peptide for secretion and additional C-terminal motifs such as RXLRs and CRNs for host targeting. It has recently been shown that oomycetes exploit non-conventional secretion mechanisms to transfer certain proteins to the extracellular environment. Other non-classical secretion systems involved in plant-fugal interaction include extracellular vesicles (EVs, Figure 1 from Samuel et al 2016 Front. Plant Sci. 6:766.). The versatility of oomycetes, fungi and bacteria allows them to associate with plants in many ways depending on whether they are biotroph, hemibiotroph, necrotroph, or saprotroph. When interacting with a live organism, a microbe will invade its plant host and manipulate its metabolisms either detrimentally if it is a pathogen or beneficially if it is a symbiote. Deciphering secretomes became a crucial biological question when an increasing body of evidence indicated that secreted proteins were the main effectors initiating interactions, whether of pathogenic or symbiotic nature, between microbes and their plant hosts. Secretomics may help to contribute to the global food security and to the ecosystem sustainability by addressing issues in i) plant biosecurity, with the design of crops resistant to pathogens, ii) crop yield enhancement, for example driven by arbuscular mycorrhizal fungi helping plant hosts utilise phosphate from the soil hence increase biomass, and iii) renewable energy, through the identification of microbial enzymes able to augment the bio-conversion of plant lignocellulosic materials for the production of second generation biofuels that do not compete with food production. To this day, more than a hundred secretomics studies have been published on all taxa and the number of publications is increasing steadily. Secretory pathways have been described in various species of microbes and/or their plant hosts, yet the functions of proteins secreted outside the cell remain to be fully grasped. This Research Topic aims at discussing how secretomics can assist the scientists in gaining knowledge about the mechanisms underpinning plant-microbe interactions.

This book is a printed edition of the Special Issue "Plant Microbe Interaction 2017" that was published in IJMS

A multiplicity of biotrophic micro-organisms interact with plants in nature, forming symbiotic relationships that range from mutualism to antagonism. Microorganisms that have adopted biotrophy as a lifestyle are able to colonize the plant and often to cross the plant cell boundaries by forming intracellular structures that are the site of nutrient uptake/exchange. To establish themselves within plant tissues, both mutualistic and pathogenic biotrophs need to overcome the plant defense response through an exchange of molecular signals. Our knowledge of the nature of these signals and their function in the interaction has rapidly increased over the last few years. This volume focuses on the genetic, molecular and cellular components involved in the communication between partners of well-known symbioses, but also reports on the advances for less studied systems.