Chromatography Of Lipids In Biomedical Research And Clinical Diagnosis

This multi-author volume provides an unprecedented in-depth coverage of the separation, identification and quantitation of simple and complex food and tissue fats and other lipids by chromatographic and mass spectrometric methods with emphasis on applications to biomedical research and clinical diagnosis. It contains a total of 13 chapters written by scientists who are internationally recognized in their specific fields. The volume covers analyses of molecular species of eicosanoids, sterols, and glycero and glycolipids in healthy and diseased human tissues and in appropriate animal models. The text complements the simpler group determinations of lipid classes commonly employed in clinical laboratories. The volume anticipates the discovery of the specific metabolism of molecular species of complex glycerolipids and provides the medical researcher and clinical investigator with the means for dealing with it.

Focusing on state-of-the-art gas chromatography (GC) and high-performance liquid chromatography, this guide discusses the theories behind, and applications of, the latest developments in chromatographic techniques - assessing the strengths and limitations of each methodology.;Presenting many detection methods for the first time, Lipid Chromatographic Analysis: provides a review of conventional column and thin-layer chromatography; examines a new GC technique to detect plasmalogen phospholipids; outlines a supercritical fluid chromatography for lipids; details the first systematic description of a microanalytical procedure for cholesterol and related compounds; explains a GC analysis of lipid breakdown products for monitoring biological processes such as ageing, carcinogenesis and mutagenesis; and describes techniques that allow for the simultaneous analysis of non-polar and polar lipids.

Applications

In this third edition, more than 40 renowned authorities introduce and update chapters on the theory, fundamentals, techniques, and instrumentation of thin-layer chromatography (TLC) and high-performance thin-layer chromatography (HPTLC), highlighting the latest procedures and applications of TLC to 19 important compound classes and coverage of TLC applications by compound type. Easily adaptable to industrial scenarios , the Handbook of Thin-Layer Chromatography, Third Edition supports practical research strategies with extensive tables of data, offers numerous figures that illustrate techniques and chromatograms, and includes a glossary as well as a directory of equipment suppliers.

Chromatography for the Analysis of Lipids focuses on the three main chromatographic techniques for analyzing lipids: thin layer (TLC), gas liquid (GLC), and high performance liquid chromatography (HPLC). It also covers supercritical fluid chromatography (SFC). Applications for each chromatographic technique are discussed with emphasis on their quantitative aspects and suitability for use, and variations in technique are covered in detail. Figures are used throughout the book to illustrate typical results, which shows end points that can be compared in your own laboratory. This important technical book will be an excellent workbench companion for chromatographers, food scientists, lipid chemists, analytical chemists, and others who use chromatographic techniques for lipid analysis.

Handbook of Chromatography: Analysis of Lipids provides a valuable review of state-of-the-art applications of chromatographic techniques (TLC, GC, HPLC) and other analytical techniques. Much of this volume is devoted to applications of HPLC (including supercritical fluid chromatography) in the analysis of lipids such as fatty acids, oxygenated fatty acids, enantiomeric acyl- and alkylglycerols, and lipoproteins. The handbook also provides extensive coverage of applications of combinations of various chromatographic techniques used in the analysis of ozonides, anacardic acids, glycerophospholipids, products of lipolysis, artifacts and contaminants in edible fats, acylated proteins, non-caloric lipids, lipophilic vitamins, acyl-Coenzyme A thioesters, dolichols, mycolic acids, technical fats and fat products, and liposomes. Handbook of Chromatography: Analysis of Lipids will be a useful reference for oil chemists, biochemists, fat science technologists, and other scientists involved in lipid research.

This book provides the most up-to-date information on a wide range of developments in chromatographic methods and applications. It presents timely, cutting-edge reviews in the fields of bio-, analytical, organic, polymer, and pharmaceutical chemistry.

The food analyst plays an important role in modern society. Stricter control over additives in food and concern about the effects of contamination of food by industrial and agricultural chemicals are among the developments which are leading to an increasing emphasis on detailed and accurate analysis of food. However. analysis of food is required for many reasons, including detection of toxic components, monitoring legislation, detecting adulteration, formulation of controlled diets, controlling formulation during product development and detecting changes in food during storage and processing. Foods comprise a complex mixture of components and food analysis requires efficient methods of separation with high sensitivity or specificity of detection. Although many food components are involatile or thermally labile and therefore not suitable for analysis by gas chromatography, other components are volatile and this technique is the preferred analytical method. Developments in methods of derivati zation, injector design and column technology have also extended the applicability of gas chromatography to the analysis of relatively involatile compounds.