Clinical And Forensic Applications Of Capillary Electrophoresis Pathology And Laboratory Medicine
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Clinical and Forensic Applications of Capillary Electrophoresis
Author | : John R. Petersen,Amin A. Mohammad |
Publsiher | : Springer Science & Business Media |
Total Pages | : 449 |
Release | : 2001-04-20 |
Genre | : Medical |
ISBN | : 9781592591206 |
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Because capillary electrophoresis (CE) is driving the automation of manual and semi-manual techniques, conserves precious samples, and minimizes the use of hazardous organic chemicals, it constitutes a powerful new methodology for the clinical laboratory. In Clinical and Forensic Applications of Capillary Electrophoresis, John R. Petersen and Amin A. Mohammad, along with a panel of leading basic and clinical investigators, review those CE methods that are now replacing many routine serum and blood tests in clinical and forensic laboratories. Major areas reviewed include the coating of columns; the analysis of serum, urine, and CSF proteins and paraproteins; abnormal hemoglobins and hemoglobin Alc; peptides, amino and organic acids; therapeutic drugs; drugs of abuse; viral load; and short tandem repeats (STR). The methods discussed include capillary zone, micellar, electrokinetic, capillary gel, and nonaqueous electrophoresis. The authors show how a variety of detection methods (UV-visible, fluorescence, and MS) can be used in conjunction with CE to detect a broad array of analytes, ranging from serum proteins to PCR products to drugs of forensic importance. Innovative and highly practical, Clinical and Forensic Applications of Capillary Electrophoresis demonstrates the power and versatility of CE-not only to develop new assays, but also to markedly simplify today's clinical and forensic laboratory methodology.
Clinical and Forensic Applications of Capillary Electrophoresis Pathology and Laboratory Medicine
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Author | : John R. Petersen,Amin A. Mohammad |
Publsiher | : Unknown |
Total Pages | : 135 |
Release | : 2001 |
Genre | : Electronic Book |
ISBN | : OCLC:748539240 |
Download Clinical and Forensic Applications of Capillary Electrophoresis Pathology and Laboratory Medicine Book in PDF, Epub and Kindle
Because capillary electrophoresis (CE) is driving the automation of manual and semi-manual techniques, conserves precious samples, and minimizes the use of hazardous organic chemicals, it constitutes a powerful new methodology for the clinical laboratory. In Clinical and Forensic Applications of Capillary Electrophoresis, John R. Petersen and Amin A. Mohammad, along with a panel of leading basic and clinical investigators, review those CE methods that are now replacing many routine serum and blood tests in clinical and forensic laboratories. Major areas reviewed include the coating of columns; the analysis of serum, urine, and CSF proteins and paraproteins; abnormal hemoglobins and hemoglobin Alc; peptides, amino and organic acids; therapeutic drugs; drugs of abuse; viral load; and short tandem repeats (STR). The methods discussed include capillary zone, micellar, electrokinetic, capillary gel, and nonaqueous electrophoresis. The authors show how a variety of detection methods (UV-visible, fluorescence, and MS) can be used in conjunction with CE to detect a broad array of analytes, ranging from serum proteins to PCR products to drugs of forensic importance. Innovative and highly practical, Clinical and Forensic Applications of Capillary Electrophoresis demonstrates the power and versatility of CE-not only to develop new assays, but also to markedly simplify today's clinical and forensic laboratory methodology.
Fundamentals of Molecular Diagnostics
Author | : David E. Bruns,Edward R. Ashwood,Carl A. Burtis |
Publsiher | : Elsevier Health Sciences |
Total Pages | : 298 |
Release | : 2007-05-25 |
Genre | : Medical |
ISBN | : 9781416037378 |
Download Fundamentals of Molecular Diagnostics Book in PDF, Epub and Kindle
This book offers an introduction to the newest, fastest-growing field in laboratory science. Explaining and clarifying the molecular techniques used in diagnostic testing, this text provides both entry-level and advanced information. It covers the principles of molecular biology along with genomes and nucleic acid alterations, techniques and instrumentation, and applications of molecular diagnostics. Written by leading experts, including Patrick Bossuyt, Angela Caliendo, Rossa W.K. Chiu, Kojo S.J. Elenitoba-Johnson, Andrea Ferreira-Gonzalez, Amy Groszbach, Sultan Habeebu, Doris Haverstick, Malek Kamoun, Anthony Killeen, Noriko Kusukawa, Y.M. Dennis Lo, Elaine Lyon, Gwendolyn McMillin, Christopher Price, James Versalovic, Cindy Vnencak-Jones, Victor Weedn, Peter Wilding, Thomas Williams, and Carl Wittwer, this book includes illustrations, tables, and a colorful design to make information easy to find and easy to use. A full-color, 4-page insert shows realistic images of the output for many molecular tests. Learning Objectives open each chapter with an overview of what you should achieve. Key Words are listed and defined at the beginning of each chapter, and are bolded in the text. Review Questions at the end of every chapter let you measure your comprehension. Advanced Concepts are included, but set apart from the rest of the text, for students who want a higher level of learning. Ethics boxes address ethical issues, allowing you to apply your knowledge to real-life scenarios. A glossary of all key words may be easily accessed in the back of the book.
Clinical Applications of Capillary Electrophoresis
Author | : Stephen M. Palfrey |
Publsiher | : Springer Science & Business Media |
Total Pages | : 215 |
Release | : 2008-02-01 |
Genre | : Medical |
ISBN | : 9781592596898 |
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In Clinical Applications of Capillary Electrophoresis, Stephen Palfrey brings together for first time a collection of detailed capillary electrophoresis protocols designed exclusively for clinical applications. Written by the leading scientists who have often perfected these methods in their own laboratories, the protocols furnish new and more powerful assays for many routine serum and blood tests now regularly performed in clinical laboratories, including urine protein analysis, hemoglobin separation, and the detection of CSF proteins, lipoproteins, myoglobin, cryoglobulins, HbA1c, and cathepsin. The protocols offered for DNA studies include double-stranded DNA analysis, the prenatal diagnosis of Down's syndrome, Rh D/d genotyping, the identification of mutated p53 oncogene, and the detection of microsatellite instability in cancers. Many of the methods can be automated to replace the more costly and labor-intensive tests that are currently used in most clinical laboratories. Clinical Applications of Capillary Electrophoresis demonstrates clearly the simplicity, versatility, and power of CE over conventional methods. It offers to beginning clinical investigators, as well as established laboratories new to the technique, a representative range of highly practical CE methods-assays that are not only certain to become ever more productive, but are already eminently useful today.
Electrophoresis Recent Advances New Perspectives and Applications
Author | : Yee-Shan Ku |
Publsiher | : BoD – Books on Demand |
Total Pages | : 102 |
Release | : 2024-01-24 |
Genre | : Science |
ISBN | : 9781837694310 |
Download Electrophoresis Recent Advances New Perspectives and Applications Book in PDF, Epub and Kindle
Electrophoresis is a classic molecular biology technique. The basic idea is to separate molecules based on their sizes and charges. Coupled with other technologies, electrophoresis is applied in various research fields to suit different purposes. This book describes and discusses the applications of electrophoresis in various research fields, including single-cell technology, veterinary diagnosis, dental research, biodiversity study, and soil research.
Capillary Gel Electrophoresis
Author | : Andras Guttman,László Hajba |
Publsiher | : Newnes |
Total Pages | : 391 |
Release | : 2021-12-04 |
Genre | : Science |
ISBN | : 9780080931357 |
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Capillary Gel Electrophoresis and Related Microseparation Techniques covers all theoretical and practical aspects of capillary gel electrophoresis. It also provides an excellent overview of the key application areas of nucleic acid, protein and complex carbohydrate analysis, affinity-based methodologies, micropreparative aspects and related microseparation methods. It not only gives readers a better understanding of how to utilize this technology, but also provides insights into how to determine which method will provide the best technical solutions to particular problems. This book can also serve as a textbook for undergraduate and graduate courses in analytical chemistry, analytical biochemistry, molecular biology and biotechnology courses. Covers all theoretical and practical aspects of capillary gel electrophoresis Excellent overview of the key applications of nucleic acid, protein and complex carbohydrate analysis, affinity-based methodologies, micropreparative aspects and related microseparation methods Teaches readers how to use the technology and select methods that are ideal for fundamental problems Can serve as a textbook for undergraduate and graduate courses in analytical chemistry, analytical biochemistry, molecular biology and biotechnology courses
Evaluation of Environmental Contaminants and Natural Products A Human Health Perspective
Author | : Ashita Sharma,Manish Kumar,Satwinderjeet Kaur ,Avinash Kaur Nagpal |
Publsiher | : Bentham Science Publishers |
Total Pages | : 296 |
Release | : 2019-06-10 |
Genre | : Science |
ISBN | : 9789811410956 |
Download Evaluation of Environmental Contaminants and Natural Products A Human Health Perspective Book in PDF, Epub and Kindle
Unbridled urbanization and development of natural land resources has led to the degradation of our surrounding environment. The air that we breathe, the water we drink and the food we eat is at risk of being contaminated with a plethora of chemical pollutants, some of them being potentially carcinogenic. This presents a challenge to human health. This book attempts to address this challenge in two parts which represent two different approaches. The first part of the book summarizes the alarming effects of environmental contaminants. Various studies depicting the direct relationship of environmental contaminants with cancer incidence have been referenced. Scientific studies have established an inverse relation between cancer and ingestion of dietary phytoconstituents (phytochemicals) in the form of fruits, vegetables and botanical herbs. Plant products as dietary supplements can suppress contaminant toxicity by regulating the resulting reactive species and also by assisting their bodily excretion through Phase 1 and Phase 2 enzyme metabolism. The second part of the book, shifts focus to phytoconstituents which, if included in diet, can prevent the harmful effects of pollutants. The text references numerous studies showing the anti-mutagenic, anti-genotoxic and anti-carcinogenic potential of many plant products. The combination of information about contemporary issues of carcinogenic contaminants in the environment coupled with the references to relevant studies in this handbook will enlighten readers studying courses in environmental chemistry, toxicology, botany, and ecology about environmental toxins and help them understand specific dietary measures known to reduce the toxic impact. Researchers in the field of nutrition can also benefit from the information provided.
Electrophoresis
Author | : Budin Michov |
Publsiher | : Walter de Gruyter GmbH & Co KG |
Total Pages | : 1122 |
Release | : 2020-09-07 |
Genre | : Science |
ISBN | : 9783110389968 |
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Electrophoresis is a straightforward but informative analytical method used in biochemistry, biology and medicine. This book combines a detailed discussion of theory and technical application with an elaborate section on troubleshooting and problem solving in electrophoresis. Therefore the book is an important guide for both students and scientists.