Comprehensive Laboratory Manual In Biology Xii

With newly introduced 2 Term Examination Pattern, CBSE has eased out the pressure of preparation of subjects and cope up with lengthy syllabus. Introducing, Arihant’s CBSE TERM II – 2022 Series, the first of its kind that gives complete emphasize on the rationalize syllabus of Class 10th & 12th. The all new “CBSE Term II 2022 – Biology” of Class 12th provides explanation and guidance to the syllabus required to study efficiently and succeed in the exams. The book provides topical coverage of all the chapters in a complete and comprehensive manner. Covering the 50% of syllabus as per Latest Term wise pattern 2021-22, this book consists of: 1. Complete Theory in each Chapter covering all topics 2. Case-Based, Short and Long Answer Type Question in each chapter 3. Coverage of NCERT, NCERT Examplar & Board Exams’ Questions 4. Complete and Detailed explanations for each question 5. 3 Practice papers base on entire Term II Syllabus. Table of Content Human Health and Diseases, Microbes in Human Welfare, Biotechnology: Principles and Processes, Biotechnology and its Applications, Organisms and Populations, Biodiversity and Its Conversation, Practice Paper (1-3)

A. Surface Chemistry 1.To prepare colloidal solution (sol) of starch, 2. To prepare a colloidal solution of egg albumin 3.To prepare colloidal solution of gum, 4. To prepare colloidal solution of aluminium hydroxide [Al(OH)3], 5.To prepare colloidal solution of ferric hydroxide [Fe(OH)3], 6.To prepare colloidal solution of arsenious sulphide [As2S3], 7. To purify a freshly prepared sol by dialysis, 8. To compare the effectiveness of different common oils (Castor oil, cotton seed oil, coconut oil, kerosene oil, mustard oil) in forming emulsions. Viva-Voce B. Chemical Kinetics 1. To study the effect of concentration on the rate of reaction between sodium thiosulphate and hydrochloric acid, 2. To study the effect of temperature on the rate of reaction between sodium thiosulphate and hydrochloric acid, 3. To study the rate of reaction of iodide ions with hydrogen peroxide at different concentrations of iodide ions, 4. To study the rate of reaction between potassium iodate (KIO3) and sodium sulphite (Na2SO3) using starch solution as indicatorl Viva-Voce C. Thermochemistry 1.Determine the enthalpy of dis solution of copper sulphate (CuSO4.5H2O) in water at Room temperature, 2. To determine the enthalpy of neutralization of the reaction between HCl and NaOH, 3. To determine enthalpy change during the interaction between acetone and chloroform Viva-Voce D. Electrochemistry 1.To study the variation of cell potential in Zn|Zn2+||Cu2+|Cu, with change in concentration of electrolytes (CuSO4 or ZnSO4) at room temperature Viva-Voce E.Chromatography 1.To separate the coloured components (pigment) present in the given extract of leaves and flowers by ascending paper chromatography and find their Rf values, 2. To separate the coloured components present in the mixture of red and blue inks by ascending paper chromatography and find their Rf values, 3.To separate Co2+ and Ni2+ ions present in the given mixture by using ascending paper chromatography and determine their Rf values Viva-Voce F. Preparation of Inorganic Compounds 1.Preparation of double salt of ferrous ammonium sulphate (Mohr’s salt) from ferrous sulphate and ammonium sulphate, 2. To prepare a pure sample of potash alum (fitkari), 3. Preparation of crystals of potassium ferric oxalate or pottasium trioxlato ferrate (III) Viva-Voce G. Preparation of Organic Compounds 1. Preparation of iodoform from ethyl alcohol or acetone, 2. Preparation of acetanilide in laboratory, 3. Preparation of b-Naphthol aniline dye, 4. To prepare a pure sample of dibenzalacetone, 5. To prepare a pure sample of p-nitro acetanilide Viva-Voce H. Tests for the Functional Groups Present in Organic Compounds Viva-Voce I. Study of Carbohydrates, Fats and Proteins 1.To study simple reactions of carbohydrate, 2. To study simple reactions of fats, 3. To study simple reactions of proteins, 4. To investigate presence of carbohydrates, fats and proteins in food stuffs Viva-Voce J. Volumetric Analysis 1. To prepare 250 ml of M/10 solution of oxalic acid, 2.To prepare 250 ml of M/10 solution of ferrous ammonium sulphate, 3. Prepare M/20 solution of oxalic acid, with its help find out the molarity and strength of the given solution of potassium permanganate, 4.Prepare M/20 solution of Mohr’s salt, using this solution determine the molarity and strength of potassium permanganate solution Viva-Voce K. Qualitative Analysis Viva-Voce INVESTIGATORY PROJECTS 1.To study the presence of oxalate ions in guava fruit at different stages of ripening. 2. To study the quantity of caseine present in different samples of milk. 3.Preparation of soyabean milk and its comparison with natural milk with respect to curd formation, effect of temperature etc.4.To study the effect of potassium bisulphite as food preservative at various concentrations. 5. To study the digestion of starch by salivary amylase and the effect of pH and temperature on it. 6. To study and compare the rate of fermentation of the following materials—wheat flour, gram flour, potato juice and carrot juice. 7.To extract essential oils present in saunf (aniseed), ajwain (corum), illaichi (cardomom).8. To detect the presence of adulteration in fat, oil and butter, 9.To investigate the presence of NO2– in brinjal.

This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein. The third edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, rather than a 4-week intensive course. The “project approach to experiments was maintained: students still follow a cloning project through to completion, culminating in the purification of recombinant protein. It takes advantage of the enhanced green fluorescent protein - students can actually visualize positive clones following IPTG induction. Cover basic concepts and techniques used in molecular biology research labs Student-tested labs proven successful in a real classroom laboratories Exercises simulate a cloning project that would be performed in a real research lab "Project" approach to experiments gives students an overview of the entire process Prep-list appendix contains necessary recipes and catalog numbers, providing staff with detailed instructions