Computational Methods For Three Dimensional Microscopy Reconstruction

Approaches to the recovery of three-dimensional information on a biological object, which are often formulated or implemented initially in an intuitive way, are concisely described here based on physical models of the object and the image-formation process. Both three-dimensional electron microscopy and X-ray tomography can be captured in the same mathematical framework, leading to closely-related computational approaches, but the methodologies differ in detail and hence pose different challenges. The editors of this volume, Gabor T. Herman and Joachim Frank, are experts in the respective methodologies and present research at the forefront of biological imaging and structural biology. Computational Methods for Three-Dimensional Microscopy Reconstruction will serve as a useful resource for scholars interested in the development of computational methods for structural biology and cell biology, particularly in the area of 3D imaging and modeling.

Three-dimensional (3D) reconstruction is the process of capturing the shape and appearance of real objects using computer vision and computer graphics. In this book, the authors present topical research in the study of the methods, applications and challenges of 3D reconstruction. Topics include 3D medical reconstruction and case studies; 3D reconstruction of coronary anatomy using invasive imaging modalities; recent advances in eel spectroscopic tomography; stereoscopic Schlieren/shadowgraph 3D reconstruction techniques; three-dimensional refractive index imaging of cells to study light scattering properties of cells and tissue; 3D imaging of material properties by combination of scanning probe microscope and ultramicrotome; 3D reconstruction and its application for maxillofacial surgery training; the automated systems of processing of the fragmented material at archaeological and craniology 3D reconstruction; three-dimensional reconstruction of an acinus for numerical and experimental studies; large scene reconstruction based on ToF cameras; and the structure and motion factorization of nonrigid objects.

Principles of Electron Optics: Second Edition, Advanced Wave Optics provides a self-contained, modern account of electron optical phenomena with the Dirac or Schrödinger equation as a starting point. Knowledge of this branch of the subject is essential to understanding electron propagation in electron microscopes, electron holography and coherence. Sections in this new release include, Electron Interactions in Thin Specimens, Digital Image Processing, Acquisition, Sampling and Coding, Enhancement, Linear Restoration, Nonlinear Restoration – the Phase Problem, Three-dimensional Reconstruction, Image Analysis, Instrument Control, Vortex Beams, The Quantum Electron Microscope, and much more. Includes authoritative coverage of many recent developments in wave electron optics Describes the interaction of electrons with solids and the information that can be obtained from electron-beam techniques Includes new content on multislice optics, 3D reconstruction, Wigner optics, vortex beams and the quantum electron microscope

Cryoelectron microscopy of biological molecules is among the hottest growth areas in biophysics and structural biology at present, and Frank is arguably the most distinguished practitioner of this art. CryoEM is likely over the next few years to take over much of the structural approaches currently requiring X-ray crystallography, because one can now get good and finely detailed images of single molecules down to as little as 200,000 MW, covering a substantial share of the molecules of greatest biomedical research interest. This book, the successor to an earlier work published in 1996 with Academic Press, is a natural companion work to our forthcoming book on electron crystallography by Robert Glaeser, with contributions by six others, including Frank. A growing number of workers will employ CryoEM for structural studies in their own research, and a large proportion of biomedical researchers will have a growing interest in understanding what the capabilities and limits of this approach are.

In 1917, Johann Radon published his fundamental work, where he introduced what is now called the Radon transform. Including important contributions by several experts, this book reports on ground-breaking developments related to the Radon transform throughout these years, and also discusses novel mathematical research topics and applications for the next century.

The Academic Job Search Handbook is the comprehensive guide to finding a faculty position in any discipline. Building on the groundbreaking success and unique offerings of earlier volumes, the fifth edition presents insightful new content on aspects of the search at all stages. Beginning with an overview of academic careers and institutional structures, it moves step by step through the application process, from establishing relationships with advisors, positioning oneself in the market, learning about job openings, preparing CVs, cover letters, and other application materials, to negotiating offers. Of great value are the sixty new sample documents from a diverse spectrum of successful applicants. The handbook includes a search timetable, appendices of career resources, and a full sample application package. This fifth edition features new or updated sections on issues of current interest, such as job search concerns for pregnant or international candidates, the use of social media strategies to address CV gaps, and difficulties faced by dual-career couples. The chapter on alternatives to faculty jobs has been expanded and presents sample résumés of PhDs who found nonfaculty positions. For more than twenty years, The Academic Job Search Handbook has assisted job seekers in all academic disciplines in the search for faculty positions at different kinds of institutions from research-focused universities to community colleges. Current faculty who used the book themselves recommend it to their own students and postdocs. The many new first-person narratives provide insight into issues and situations candidates may encounter such as applying for an international job, combining parenting with an academic career, going from an administrative job to a faculty position, and seeking faculty positions as a same-sex couple.

This unique resource details the theory, working methods, and applications of electron tomographic techniques for imaging asymmetric, noncrystalline biological specimens.

Readership: Academics, researchers, industrialists, postgraduate and graduate students in databases, fuzzy logic, machine vision/pattern recognition, neural networks, bioengineering, electrical & electronic engineering, and bioinformatics.Key Features: Provides a significant and uniquely comprehensive reference source for research workers and practitioners Features 130 contributors from 27 countries, among the foremost authorities in industry, government and academia Institutions, laboratories and individuals involved in the area of medical imaging should possess this setKeywords:Medical Imaging;Systems Technology;Cardiovascular Systems;Brain Systems;General Anatomy;Modalities;Diagnosis Optimization Methods;Computational Methods