Gene Expression And Regulation In Cultured Cells

This volume provides a broad, state-of-the-art coverage of diverse technical topics in gene expression in mammalian cells, including the development of vectors for production of proteins in cultured cells, in transgenic animals, vaccination, and gene therapy; progress in methods for the transfer of genes into mammalian cells and the optimization and monitoring of gene expression; advances in our understanding and manipulation of cellular biochemical pathways that have a quantitative and qualitative impact on mammalian gene expression; and the large-scale production and purification of proteins from cultured cells.

"Central dogma" was presented by Dr. Francis Crick 60 years ago. The information of nucleotide sequences on DNAs is transcribed into RNAs by RNA polymerases. We learned the mechanisms of how transcription determines function of proteins and behaviour of cells and even how it brings appearances of organisms. This book is intended for scientists and medical researchers especially who are interested in the relationships between transcription and human diseases. This volume consists of an introductory chapter and 14 chapters, divided into 4 parts. Each chapter is written by experts in the basic scientific field. A collection of articles presented by active and laboratory-based investigators provides recent advances and progresses in the field of transcriptional regulation in mammalian cells.

This cutting-edge text will be a valuable resource for scientists and aspiring graduate students interested in the intersection of RNAi, miRNA, and stem cell molecular biology and exciting areas of research such as regenerative medicine and cancer.

The Second Edinburgh International Workshop was held in September, 1984 and took as its topic the coordinated regulation of gene expression. The intention of this series of workshops is to promote exchange of ideas and data between scientists and clinicians whose interests span molecular and cell biology, development and differentiation, oncology, and genetic and developmental pathologies. It is hoped that such interdisciplinary discussions may give rise to fruitful insights. The meetings are structured to give ample time for discussion after each formal presentation and culminate in a session of general discussion which is reported at the end of the volume of proceedings. We are very grateful to the participants, all of whom participated in the discussion and whose contributions were essential to the success of the meeting. Novel ideas were often put forward and explored thoroughly from different angles. We normally expect to achieve quite rapid publication of the proceedings of the meeting and are grateful to authors who produced typescripts of their contributions expeditiously, but, as may sometimes be the case with multi-author works, some contributors had difficulty in meeting schedules for submitting manuscripts or corrections of the text of the discussion, and in one case we have been unable to publish any record of the contribution. Our committment to the publication of the discussion, allowing participants to make corrections to the transcript of the session, such as insertion of references and clarification of oral contributions, has also imposed some delay.