Genetic Engineering And In Vitro Culture Of Crop Legumes

An introductory chapter provides an up-to-date review of biotechnology and genetic engineering for crop legumes: strategy, techniques and goals. Following chapters examine each of major category: economic and nutritional importance, applicable genetic engineering techniques, and feasible objectives for improvement. Special attention is given to soybeans, the most important of the legumes. The text is well illustrated and carefully organized for easy reference.

The contributions of plant genetics to the production of higher yielding crops of superior quality are well documented. These successes have been realized through the application of plant breeding techniques to a diverse array of genetically controlled traits. Such highly effective breeding procedures will continue to be the primary method employed for the development of new crop cultivars; however, new techniques in cell and molecular biology will provide additional approaches for genetic modification. There has been considerable speculation recently concerning the potential impact of new techniques in cell and molecular biology on plant improvement. These genetic engineering techniques should offer unique opportunities to alter the genetic makeup of crops if applied to existing breeding procedures. Many questions must be answered in order to identify specific applications of these new technologies. This search for applications will require input from plant scientists working on various aspects of crop improvement. This volume is intended to assess the interrelationships between conventional plant breeding and genetic engineering.

Legumes include many very important crop plants that contribute very critical protein to the diets of both humans and animals around the world. Their unique ability to fix atmospheric nitrogen in association with Rhizobia enriches soil fertility, and establishes the importance of their niche in agriculture. Divided into two volumes, this work presents an up-to-date analysis of in vitro and recombinant DNA technologies for the improvement of grain, forage and tree legumes. Volume 10B presents the current state and future prospects of in vitro regeneration and genetic transformation expression and stability of transgenes modification of traits in almost all the important legumes, for example: soybean; peanut; pea; french bean; chick pea; pigeon pea; cowpea; mung bean; black gram; azuki bean; lentil; Lathyrus; lupinus; Lotus spp; Medicago spp; Trifolium spp; Winged bean; Guar; and tree legumes for their improvement.

A comprehensive and groundbreaking collection of ideas for plant improvement Most of the world’s supply of legumes is cultivated under adverse conditions that make this commercially important crop susceptible to the vagaries of nature and damaging stresses. Genetic manipulation has become a proven way for cultivators to battle these problems; but there has been a lack of reliable information on the practical aspects of this promising method. Handbook of New Technologies for Genetic Improvement of Legumes fills this void by providing a vast array of groundbreaking essays from some of the discipline’s most renowned experts. This unique collection is your single source for not only the most up-to-date accounts of genetic manipulation employed today, but also ideas for realistic and practical uses. Comprehensive in its approach, Handbook of New Technologies for Genetic Improvement of Legumes presents the latest research on all aspects of genetically modified legume production. With contributions from over 90 international experts, this eye-opening resource offers an in-depth exploration of key topics such as, genetic transformation technology, suppression-subtractive hybridization, c-DNA micro arrays, molecular mapping, tagging regulatory elements, and real-time PCR. Additionally, this text features a wealth of detailed references and informative tables to clearly explain pertinent data. Chapters in Handbook of New Technologies for Genetic Improvement of Legumes detail both family- and species-specific topics such as: • agrobacterium-mediated transformation in trifolium and medicago • biotechnological approaches to soybean improvement • transgenic approaches for enhancement of vitamin E • particle bombardment and SAAT approaches • agrobacterium tumefaciens-mediated transformation and in vitro regeneration • plant regeneration via organogenesis, somatic embryogenesis, and protoplast culture • somaclonal variation and its exploitation • cotyledonary node and embryo axes as explants • in planta transformation of pisum sativum l. • de novo regeneration protocol • reverse genetic approaches in medicago truncatula • tagging regulatory elements in plants • gene expression analysis with DNA array technology • advent of diverse DNA markers to decipher genome sequence polymorphism • defensins in legumes • legume-nematode interaction with reference to model legumes • and much, much more! Handbook of New Technologies for Genetic Improvement of Legumes is an invaluable resource for researchers and educators in the area of plant genetic manipulation and plant biotechnology as well as scientists, educators, and graduate students focusing on the genetic manipulation of legumes.

The applications of biotechnology to the improvement of horticultural crops have been considerable. In vitro propagation technologies, commonly referred to as micropropagation, are the methods of choice for propagating a wide range of horticultural crops. This book explores this subject.

Plant Cell and Tissue Culture gives an exhaustive account of plant cell culture and genetic transformation, including detailed chapters on all major field and plantation crops. Part A presents a comprehensive coverage of all necessary laboratory techniques for the initiation, nutrition, maintenance and storage of plant cell and tissue cultures, including discussions on these topics, as well as on morphogenesis and regeneration, meristem and shoot tip culture, plant protoplasts, mutant cell lines, variation in tissue cultures, isogenic lines, fertilization control, cryopreservation, transformation, and the production of secondary metabolites. Part B then proceeds into detail on the specific in vitro culture of specific crops, including cereals, legumes, vegetables, potatoes, other roots and tubers, oilseeds, temperate fruits, tropical fruits, plantation crops, forest trees and ornamentals. Plant Cell and Tissue Culture is, and is likely to remain, the laboratory manual of choice, as well as a source of inspiration and a guide to all workers in the field.

Biotechnology Has Made Significant Advances In Recent Years And Emerged As A Frontline Area Of Research And Development, With An Overwhelming Impact On The Society. Plant Biotechnology Is A Engine For Green To Evergreen Revolution. It Has Shown Great Promise In Recent Years For Improving Crop Productivity, Reducing Environmental Pollution And Improving The Quality Of Agricultural Produce. This Book Contains 17 Chapters Written By Leading Experts In The Discipline Incorporating Recent Developments In The Subject. Major Section Includes Articles On Plant Biotechnology. Topic Covering Information On Genetic Modification In Plants - A Biotechnological Scenario; Genetic Transformation In Leguminous Crops; Role Of Biotechnology In Ornamental Crops; Genetic Engineering For Major Abiotic Stresses Tolerance In Crop Plants; Transgenic Technology For Future; Molecular Markers In Plant Genome Analysis And Molecular Markers For Assessing Genetic Diversity In Coconut Palm. Topics On Micropropagation Of Prosopis, Callusing Response And Direct In Vitro Regeneration Of Nodal Explants; In Vivo Propagation And Conservation Of Pogostemon; In Vivo & In Vitro Comparison Of Bioactive Substances From Bacopa, Centella And Convolvulus Related To Memory Improvement Provide Useful Information On Application Of Tissue Culture Technique In Biotechnology. Articles On Microfluidics; Tannase: A Versatile Enzyme For Future'S Biotechnology; Bioplastics- Biotechnological Solution For Undegradable Plastic Wastes; Peptide Nucleic Acid: Prospects Of 21St Century & Genetic Engineering Of Plants For Environmental Cleaning Up Are Some Of The Important Areas Which Have Been Specifically Addressed In Order To Make This Book More Distinct And Relevant, In The Present Day Context. This Book Will Be Useful To Biotechnologists, Agriculture Scientists, Researches, Teachers & Students Of Plant Science.

The world's most comprehensive, well documented and well illustrated book on this subject. With extensive subject and geographic index. 152 photographs and illustrations - mostly color, Free of charge in digital format on Google Books.