Genetic Engineering Fundamentals

This important reference/text provides technologists with the basic informationnecessary to interact scientifically with molecular biologists and get involved in scalinguplaboratory procedures and designing and constructing commercial plants.Requiring no previous training or experience in biology, Genetic EngineeringFundamentals explains the biological and chemical principles of recombinant DNAtechnology ... emphasizes techniques used to isolate and clone specific genes frombacteria, plants, and animals, and methods of scaling-up the formation of the geneproduct for commercial applications ... analyzes problems encountered in scaling-upthe microprocessing of biochemical procedures . .. includes an extensive glossary andnumerous illustrations ... identifies other resource materials in the field ... and more.Presenting the fundamentals of biochemistry and molecular biology to workers andstudents in other fields, this state-of-the-art reference/text is essentiai reading fortechnologists in chemistry and engineering; biomedical, chemical, electrical andelectronics, industrial, mechanical, manufacturing, design, plant, control, civil, genetic,and environmental engineers; chemists, botanists, and zoologists; and advancedundergraduate and graduate courses in engineering, biotechnology, and industrialmicrobiology.

This book explains the biological and chemical principles of recombinant DNA technology. It emphasizes techniques used to isolate and clone specific genes from bacteria, plants, and animals, and methods of scaling-up the formation of the gene product for commercial applications.

Leading scientists from different countries around the world contributed valuable essays on the basic applications and safety, as well as the ethical and moral considerations, of the powerful genetic engineering tools now available for modifying the molecules, pathways, and phenotypes of species of agricultural, industrial and even medical importance. After three decades of perfecting such tools, we now see a refined technology, surprisingly unexpected applications, and matured guidelines to avoid unintentional damage to our and other species, as well as the environment, while trying to contribute to solve the biological, medical and technical challenges of society and industry. Chapters on thermo-stabilization of luciferase, engineering of the phenylpropanoid pathway in a species of high demand for the paper industry, more efficient regeneration of transgenic soybean, viral resistant plants, and a novel approach for rapidly screening properties of newly discovered animal growth hormones, illustrate the state-of-the-art science and technology of genetic engineering, but also serve to raise public awareness of the pros and cons that this young scientific discipline has to offer to mankind.

Compiles Articles For The Benefit Of Molecular Geneticists, Genetic Engineers, And Biotechnologists So As To Help The Abreast Of Latest Developments In These Fields. Useful For Students, Researchers, Faculty Members And Pharmaceutical Industry Both In India And Abroad. Covers Genes, Dna Structure And Biosynthesis, Rna Structure And Biosynthesis, Proteomic Structure And Biosynthesis, Gene Reputation And Exposition, Dna Technology, Dna Application, Genomics, Proteomics, Gene Engineering Etc.

Without sufficient knowledge of classical and molecular genetics as well as of genetic engineering, it is not possible to fully unterstand biotechnological processes. Clear, concise and comprehensive, Volume 2 gives scientists all the information they need to carry out research and production in this field. In Part I of the book the classical genetic techniques relevant to biotechnology are presented. Part II highlights the recent advances in molecular genetics which have revolutionized our knowledge in the field. Part III deals with the genetic engineering of microorganisms, and Parts IV and V concentrate on plants and animals - multicellular organsims which require special genetic engineering techniques. In the final Part VI biosafety concepts in modern biotechnology are treated. Topics included are: Mutagenesis/ Genetic Exchange Processes/ Cell Fusion/ Gene Mapping/ Transposable Elements/ Gene Expression/ Synthesis of Oligonucleotides/ Polymerase Chain Reaction (PCR)/ Transposons/ Transformation Vectors/ Recombinant Proteins/ Gene Amplification/ Transgenic Plants and Animals/ Biosafety Concepts

A unique resource for the next generation of biotech innovators Enabling everything from the deciphering of the human genome to environmentally friendly biofuels to lifesaving new pharmaceuticals, biotechnology has blossomed as an area of discovery and opportunity. Modern Biotechnology provides a much-needed introduction connecting the latest innovations in this area to key engineering fundamentals. With an unmatched level of coverage, this unique resource prepares a wide range of readers for the practical application of biotechnology in biopharmaceuticals, biofuels, and other bioproducts. Organized into fourteen sections, reflecting a typical semester course, Modern Biotechnology covers such key topics as: Metabolic engineering Enzymes and enzyme kinetics Biocatalysts and other new bioproducts Cell fusion Genetic engineering, DNA, RNA, and genes Genomes and genomics Production of biopharmaceuticals Fermentation modeling and process analysis Taking a practical, applications-based approach, the text presents discussions of important fundamentals in biology, biochemistry, and engineering with relevant case studies showing technology applications and manufacturing scale-up. Written for today's wider, more interdisciplinary readership, Modern Biotechnology offers a solid intellectual foundation for students and professionals entering the modern biotechnology industry.