Genome Editing For Precision Crop Breeding

Genome editing is rapidly transforming plant research. The technique offers unparalleled precision in breeding without the need to introduce foreign DNA into plants. CRISPR/Cas systems have established themselves as the leading technique in genome editing. This collection takes stock of the wealth of research on these techniques and their potential in crop breeding in improving traits such as yield, disease resistance, drought tolerance and nutrient use efficiency. Part 1 of this volume reviews advances in gene editing techniques such as TALENS and zinc finger nucleases, double-strand break repair techniques, insertion-based genome edits, base editing, guide RNAs, gRNA/Cas9 constructs and CRIST/Cas off targeting. Chapters also discuss advances in screening plants as well as regulatory issues. Part 2 surveys applications of gene editing in key cereal including wheat, barley, maize, rice and sorghum. Other chapters cover crops such brassicas, potato and tomato.

Part 1 of this volume reviews advances in gene editing techniques such as insertion-based genome edits, base editing, guide RNAs and CRISPR/Cas off targeting. Part 2 surveys applications of gene editing in key cereal and vegetable crops.

Global food security is increasingly challenging in light of population increase, the impact of climate change on crop production, and limited land available for agricultural expansion. Plant breeding and other agricultural technologies have contributed considerably for food and nutritional security over the last few decades. Genetic engineering approaches are powerful tools that we have at our disposal to overcome substantial obstacles in the way of efficiency and productivity of current agricultural practices. Genome engineering via CRISPR/Cas9, Cpf1, base editing and prime editing, and OMICs through genomics, transcriptomics, proteomics, phenomics, an metabolomics have helped to discover underlying mechanisms controlling traits of economic importance. Principle and Practices of OMICs and Genome Editing for Crop Improvement provides recent research from eminent scholars from around the world, from various geographical regions, with established expertise on genome editing and OMICs technologies. This book offers a wide range of information on OMICs techniques and their applications to develop biotic, abiotic and climate resilient crops, metabolomics and next generation sequencing for sustainable crop production, integration bioinformatics, and multi-omics for precision plant breeding. Other topics include application of genome editing technologies for food and nutritional security, speed breeding, hybrid seed production, resource use efficiency, epigenetic modifications, transgene free breeding, database and bioinformatics for genome editing, and regulations adopted by various countries around globe for genome edited crops. Both OMICs and genome editing are vigorously utilized by researchers for crop improvement programs; however, there is limited literature available in a single source. This book provides a valuable resource not only for students at undergraduate and postgraduate level but also for researchers, stakeholders, policy makers, and practitioners interested in the potential of genome editing and OMICs for crop improvement programs.

This book emphasizes on cutting-edge next-generation smart plant breeding approaches for maximizing the use of genomic resources generated by high-throughput genomics in the post-genomic era. Through this book the readers would learn about the recent development in the genomic approaches such as genotype by sequencing (GBS) for genomic analysis (SNPs, Single Nucleotide Polymorphism), whole-genome re-sequencing (WGRS) and RNAseq for transcriptomic analysis (DEGs, Differentially Expressed Genes). To maximize the genetic gains in the cereal/food crops, the book covers topics on transgenic breeding, genome editing, high-throughput phenotyping, reliable/precision phenotyping and genomic information-based analysis. In the era of climate change and the ever-increasing population, food security and nutritional security are the primary concern of plant breeders, growers, and policymakers to address the UN’s sustainable development goals. Chapters of this book cohere around these goals and covers techniques such as (QTL mapping, association studies, candidate gene identification), omics, RNAi [through micro RNA (miRNA), small interfering RNA (siRNA) and artificial micro RNA (amiRNA)]. It also covers other genomic techniques like antisense technology, genome editing (CRISPR/cas9, base editing) and epigenomics that assist the crop improvement programmes to fulfil the UNs sustainable development goals. It explores the influence of rapidly available sequencing data assisting in the next generation breeding programmes. This volume is a productive resource for the students, researchers, scientists, teachers, public and private sector stakeholders involved in the genetic enhancement of cereal crops.

Plant breeders have used mutagenic agents to create variability for their use in crop improvement. However, application of mutagenic agents has its own drawbacks, such as non-specificity and random nature, simultaneous effect on large numbers of genes, and induction of chromosomal aberrations. To overcome these limitations, several genome editing systems have been developed with the aid of cutting-edge technology rooted in the expertise of several research fields. Molecular Plant Breeding and Genome Editing Tools for Crop Improvement is a pivotal reference source that provides an interdisciplinary approach to crop breeding through genetics. Featuring coverage of a broad range of topics including software, molecular markers, and plant variety identification, this book is ideally designed for agriculturalists, biologists, engineers, advocates, policymakers, researchers, academicians, and students.