Handbook For Rhizobia

Rhizobia are bacteria which inhabit the roots of plants in the pea family and "fix" atmospheric nitrogen for plant growth. They are thus of enormous economic importance internationally and the subject of intense research interest. Handbook for Rhizobia is a monumental book of practical methods for working with these bacteria and their plant hosts. Topics include the general microbiological properties of rhizobia and their identification, their potential as symbionts, methods for inoculating rhizobia onto plants, and molecular genetics methods for Rhizobium in the laboratory. The book will be invaluable to Rhizobium scientists, soil microbiologists, field and laboratory researchers at agricultural research centers, agronomists, and crop scientists.

General information on the symbiotic nitrogen fixation. Isolation, identification and counting of rhizobia. Production of an inoculant and inoculation of legumes. Experiments.

The cultivation, isolation and maintenance of rhizobia; Cultivation; Isolation; Maintenance; The qualitative characteristics of rhizobia; Examination with the light microscope; Cultural and metabolic characteristics; Antigenic properties; Bacteriophage and lysogeny; Recognition of rhizobia; Enumeration; Determination of total growth; Counts of viable rhizobia; The assessment of nodulation and nitrogen fixation; Methods for greenhouse and light room; Field trials; Assessment of the need for legume inoculation; The production, control and use of legume inoculants.

In 2002, sixty international specialists met to discuss problems of high P-unavailability as a soil nutrient for crops, and the hazards of increased phosphate input to aquatic habitats from industrial and mining activities, sewage disposal, detergents, and other sources. Among the presentations were updated solutions to enhance P-uptake by plants, bioremediation potential in the rehabilitation of ecosystems, taxonomic characterization interactions with mycorrizae, the physiological and molecular basis of PSM, and more.

This volume details techniques involved in the study of beneficial microbes in agricultural microbiology towards enhancing global agricultural productivity. Chapters cover a wide range of basic and advanced techniques associated with research on isolation of agriculturally important microbes, identification, biological nitrogen fixation, microbe mediated plant nutrient use efficiency, biological control of plant diseases and pests. Authoritative and cutting-edge, Practical Handbook on Agricultural Microbiology aims to be a useful practical guide to researches to help further their study in this field.

Sharply focused, up-to-date information on microbial biofertilizers—including emerging options such as Piriformospora indica and Matsutake The Handbook of Microbial Biofertilizers provides in-depth coverage of all major microbial biofertilizers (rhizobia, arbuscular mycorrhizal fungi, and cyanobacteriaas well as new and emerging growth promoters (endophytes). It examines the role of microbes in growth promotion, bioprotectors, and bioremidiators, and presents protocols and practical strategies for using microbes in sustainable agriculture. An abundance of helpful charts, tables, and figures make complex information easy to access and understand. In this first-of-its-kind volume, contributors from 11 countries and several continents address important issues surrounding microbial biofertilizers, including: the rhizobium-host-arbuscular mycorrhizal tripartite relationship mycorrhiza as a disease suppresser and stress reducer mycorrhiza helping bacteria the impact of functional groups of soil microorganisms on nutrient turnover PBPRs as biofertilizers and biopesticides the potential of wild-legume rhizobia for use as a biofertilizers the expanding role of blue-green algae in sustainable agriculture the role of microbial fertilizers in sustainable plant production new and emerging endophytes the commercial potential of biofertilizers In this young century, the use of biofertilizers is already growing rapidly. It has been recognized that these environment-friendly bioprotectors, growth boosters, and remediators are essential for soil/plant health. The Handbook of Microbial Biofertilizers is designed to fit the expanding information needs of current and future biotechnologists, microbiologists, botanists, agronomists, environmentalists, and others whose work involves sustained agriculture.

Sustainability has a major part to play in the global challenge of continued development of regions, countries, and continents all around the World and biological nitrogen fixation has a key role in this process. This volume begins with chapters specifically addressing crops of major global importance, such as soybeans, rice, and sugar cane. It continues with a second important focus, agroforestry, and describes the use and promise of both legume trees with their rhizobial symbionts and other nitrogen-fixing trees with their actinorhizal colonization. An over-arching theme of all chapters is the interaction of the plants and trees with microbes and this theme allows other aspects of soil microbiology, such as interactions with arbuscular mycorrhizal fungi and the impact of soil-stress factors on biological nitrogen fixation, to be addressed. Furthermore, a link to basic science occurs through the inclusion of chapters describing the biogeochemically important nitrogen cycle and its key relationships among nitrogen fixation, nitrification, and denitrification. The volume then provides an up-to-date view of the production of microbial inocula, especially those for legume crops.