Handbook Of Molecular And Cellular Methods In Biology And Medicine

Since the publication of the best-selling Handbook of Molecular and Cellular Methods in Biology and Medicine, the field of biology has experienced several milestones. Genome sequencing of higher eukaryotes has progressed at an unprecedented speed. Starting with baker's yeast (Saccharomyces cerevisiae), organisms sequenced now include human (Homo sa

Several milestones in biology have been achieved since the first publication of the Handbook of Molecular and Cellular Methods in Biology and Medicine. This is true particularly with respect to genome-level sequencing of higher eukaryotes, the invention of DNA microarray technology, advances in bioinformatics, and the development of RNAi technology. Now in its third edition, this volume provides researchers with an updated tool kit that incorporates conventional as well as modern approaches to tackle biological and medicinal research in the post-genomics era. Significantly revised to address these recent changes, the editors have evaluated, revised, and sometimes replaced protocols with more efficient, more reliable, or simpler ones. The book has also been reorganized with section headings focusing on different biological levels connected to one another, taking into account the central dogma of biology (DNA → RNA → protein → metabolites). The book first explores traditional approaches and then moves to the modern "omics" approaches, including genomics, proteomics, and metabolomics. It also discusses the manipulation of biological systems (including RNAi) and macromolecular analyses, focusing on the use of microscopy. In each chapter, various notes and cautionary considerations are presented for potentially hazardous reagents. Filled with diagrams, tables, and figures to clarify methods, most chapters also contain Troubleshooting Guides indicating problems, possible causes, and solutions that may be incurred in carrying out the procedures. Researchers and scientists who master the techniques in this book are putting themselves at the cutting edge of biological and medicinal research.

Many of the available books in cellular and molecular biology fail to explain the principles and methods described. Handbook of Molecular and Cellular Methods in Biology and Medicine fills in these gaps. Not only do the authors describe the many methods and techniques most commonly used, but they also discuss why you would use each particular method. This handbook covers a wide range of techniques and methods that have been developed and extensively tested. Described in a logical framework of 21 chapters, methods include isolation and purification of DNA, RNA, and proteins; preparation of hybridization probes; electrophoresis and blotting techniques; construction of cDNA and genomic DNA libraries; DNA sequencing; PCR technology; DNA recombination and mutagenesis; transcription in vitro and translation in vitro; gene transfer and analysis of gene expression in transgenic animals and plants; cell and tissue cultures; and microscopy, as well as bioseparation techniques and preparation of monoclonal and polyclonal antibodies. The authors provide descriptive illustrations for each method. Step-by-step instructions allow you to use this book in the lab while performing the various techniques. Each chapter provides the principles behind the method, easy-to-follow instructions, the reagents and materials required, and references for further study. Many chapters also include a troubleshooting guide, cautioning what to avoid and indicating steps that require extra care. Every biologist needs this book on their lab bench!

Many of the available books in cellular and molecular biology fail to explain the principles and methods described. Handbook of Molecular and Cellular Methods in Biology and Medicine fills in these gaps. Not only do the authors describe the many methods and techniques most commonly used, but they also discuss why you would use each particular method. This handbook covers a wide range of techniques and methods that have been developed and extensively tested. Described in a logical framework of 21 chapters, methods include isolation and purification of DNA, RNA, and proteins; preparation of hybridization probes; electrophoresis and blotting techniques; construction of cDNA and genomic DNA libraries; DNA sequencing; PCR technology; DNA recombination and mutagenesis; transcription in vitro and translation in vitro; gene transfer and analysis of gene expression in transgenic animals and plants; cell and tissue cultures; and microscopy, as well as bioseparation techniques and preparation of monoclonal and polyclonal antibodies. The authors provide descriptive illustrations for each method. Step-by-step instructions allow you to use this book in the lab while performing the various techniques. Each chapter provides the principles behind the method, easy-to-follow instructions, the reagents and materials required, and references for further study. Many chapters also include a troubleshooting guide, cautioning what to avoid and indicating steps that require extra care. Every biologist needs this book on their lab bench!

Since the publication of the best-selling Handbook of Molecular and Cellular Methods in Biology and Medicine, the field of biology has experienced several milestones. Genome sequencing of higher eukaryotes has progressed at an unprecedented speed. Starting with baker's yeast (Saccharomyces cerevisiae), organisms sequenced now include human (Homo sapiens), model crucifer (Arabidopsis thaliana), and rice (Oryza sativa). The invention of DNA microarray technology and advances in bioinformatics have generated vast amounts of genomic data. Reflecting these revolutionary advances Handbook of Molecular and Cellular Methods in Biology and Medicine, Second Edition documents conventional and modern approaches to tackle scientific research in the post-genomics era. Maintaining the step-by-step format that popularized the first edition, each chapter provides the principles behind the featured method, a detailed description of each protocol, applications of the protocol to different systems, and references for further study. Handbook of Molecular and Cellular Methods in Biology and Medicine, Second Edition now includes: New protocols in all chapters, including alternative protocols In vitro transcription methods Analysis of DNA sequences New bioseparation techniques New chapters covering: mRNA differential display Inhibition of gene expression In situ hybridization (Localization of gene expression) Combinatorial techniques Computational data mining methods applied to combinatorial chemistry libraries With this book at hand, researchers, teachers, and students can understand and utilize the major techniques and methods currently employed in cellular and molecular biology.

Several milestones in biology have been achieved since the first publication of the Handbook of Molecular and Cellular Methods in Biology and Medicine. This is true particularly with respect to genome-level sequencing of higher eukaryotes, the invention of DNA microarray technology, advances in bioinformatics, and the development of RNAi technology

John Walker and Ralph Rapley have collected a wide-ranging group of molecular and biochemical techniques that are the most frequently used in medical and clinical research, especially diagnostics. The authors-well-established investigators who run their own research programs and use the methods on a regular basis-outline the practical procedures for using them and describe a variety of pertinent applications. Among the technologies presented are southern and western blotting, electrophoresis, PCR, cDNA and protein microarrays, liquid chromatography, in situ hybridization, karyotyping, flow cytometry, bioinformatics, genomics, and ribotyping. The applications include assays for mutation detection, mRNA analysis, chromosome translocations, inborn errors of metabolism, protein therapeutics, and gene therapy.

Recent advances in the biosciences have led to a range of powerful new technologies, particularly nucleic acid, protein and cell-based methodologies. The most recent insights have come to affect how scientists investigate and define cellular processes at the molecular level. This book expands upon the techniques included in the first edition, providing theory, outlines of practical procedures, and applications for a range of techniques. Written by a well-established panel of research scientists, the book provides an up-to-date collection of methods used regularly in the authors’ own research programs.