Handbook Of Thin Layer Chromatography

In this third edition, more than 40 renowned authorities introduce and update chapters on the theory, fundamentals, techniques, and instrumentation of thin-layer chromatography (TLC) and high-performance thin-layer chromatography (HPTLC), highlighting the latest procedures and applications of TLC to 19 important compound classes and coverage of TLC applications by compound type. Easily adaptable to industrial scenarios , the Handbook of Thin-Layer Chromatography, Third Edition supports practical research strategies with extensive tables of data, offers numerous figures that illustrate techniques and chromatograms, and includes a glossary as well as a directory of equipment suppliers.

In this third edition, more than 40 renowned authorities introduce and update chapters on the theory, fundamentals, techniques, and instrumentation of thin-layer chromatography (TLC) and high-performance thin-layer chromatography (HPTLC), highlighting the latest procedures and applications of TLC to 19 important compound classes and coverage of TLC applications by compound type. Easily adaptable to industrial scenarios , the Handbook of Thin-Layer Chromatography, Third Edition supports practical research strategies with extensive tables of data, offers numerous figures that illustrate techniques and chromatograms, and includes a glossary as well as a directory of equipment suppliers.

This book gives a practical introduction to one of the more popular separation techniques. Readers will learn to perform separations and will develop the ability to make an educated guess as to what the conditions will be to separate a new mixture of compounds. The authors provide classes of compound and background theory that quickly develop the skills of the student learning thin layer chromatography. Chapter coverage includes stationary phase, mobile phase, practical techniques, applications, recent developments, and advantages and disadvantages of thin layer chromatography. It also includes a bibliography of texts providing additional separations for further study.· Stationary Phase· Mobile Phase· Sample· Practical Techniques· Applications· Recent Developments· Advantages and Disadvantages of Tlc· Self Assessment Questions and Responses · Units of Measurement

Written by over 40 internationally acclaimed authorities on thin-layer chromatography (TLC), this comprehensive Second Edition presents the latest techniques, instrumentation, and applications of overpressurized, rotational, and high-performance quantitative TLC. Offering a systematic approach to TLC, the Handbook of Thin-Layer Chromatography, Second Edition contains new, practical information on the detection, identification, and documentation of chromatograph zones ... optical quantitation ... flame ionization detection ... automation and robotics ... nucleic acid derivatives ... and more.

Thin-layer chromatography has become so widely known in the space of a few years that it has proved necessary to gather into book form and thus make generally accessible the experimental material previously only available in isolated publications. As thin-layer chromatography can be used both for organic and inorganic matter as well as on quantities ranging from the nanogram to the microgram, it is impossible for anyone individual to possess sufficient laboratory experience or overall knowledge to produce a practical handbook that will be of real assistance to be ginner and specialist alike. For this reason, an international group was formed, who made it their task to produce the best possible treatise. In view of the present stage of development reached by thin-Iaycr chromatography, it seems specially apt that the authors should include yet unpublished work of their own. As thin-layer chromatography is used in many different fields in natural science and medicine, the kind of brief description of materials intelligible only to the expert has been avoided. The short guides to the chemical properties of the groups to be separated, their names, and relevant bibliographic details should facilitate introductory studies arid make possible a close acquaintance with the material in hand. It also seemed advisable to give brief details of the analytical classification of material, which is so often necessary. Although the classification used may appear unusual, it is in fact pre-eminently suitable to thin-layer chromatography.

The present edited book is the presentation of 18 in-depth national and international contributions from eminent professors, scientists and instrumental chemists from educational institutes, research organizations and industries providing their views on their experience, handling, observation and research outputs on HPTLC, a multi-dimensional instrumentation. The book describes the recent advancements made on TLC which have revolutionized and transformed it into a modern instrumental technique HPTLC. The book addresses different chapters on HPTLC fundamentals: principle, theory, understanding; instrumentation: implementation, optimization, validation, automation and qualitative and quantitative analysis; applications: phytochemical analysis, biomedical analysis, herbal drug quantification, analytical analysis, finger print analysis and potential for hyphenation: HPTLC future to combinatorial approach, HPTLC-MS, HPTLC-FTIR and HPTLC-Scanning Diode Laser. The chapters in the book have been designed in such away that the reader follows each step of the HPTLC in logical order.

Thin-Layer Chromatography (TLC) is a modern, reliable tool that complements other chromatographic techniques. This book provides a practical guide to the basic principles, procedures and pitfalls on the practical application of TLC. Thin Layer Chromatography: A Modern Practical Approach offers a sequence of chapters following the steps of the technique as the chromatographer would follow them. The chapters provide a choice of sorbent best suited to the separation intended, followed by pre-treatment required for the sample, applying the sample to the sorbent layer, development procedure, visualisation and detection, and finally quantification. Imaging and hyphenation techniques are described. The reasons why recommendations are made for specific and more general methods are covered. The book also provides an overview of some recent developments in the field.