In Situ Hybridization In Light Microscopy
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In Situ Hybridization in Light Microscopy
Author | : Gerard Morel,Annie Cavalier |
Publsiher | : CRC Press |
Total Pages | : 346 |
Release | : 2000-09-28 |
Genre | : Science |
ISBN | : 9781420041828 |
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In Situ hybridization allows the visualization of specific DNA/RNA sequences in individual cells in tissue sections, single cells, or chromosome preparations, and is an especially important method for studying DNA and RNA in heterogeneous cell populations. This book delves into in situ hybridization methods through the use of light microscopy used
In Situ Hybridization in Electron Microscopy
Author | : Gerard Morel,Annie Cavalier,Lynda Williams |
Publsiher | : CRC Press |
Total Pages | : 590 |
Release | : 2001-01-30 |
Genre | : Science |
ISBN | : 9781000611397 |
Download In Situ Hybridization in Electron Microscopy Book in PDF, Epub and Kindle
In situ hybridization is a technique that allows for the visualization of specific DNA and RNA sequences in individual cells, and is an especially important method for studying nucleic acids in heterogeneous cell populations. in situ Hybridization in Electron Microscopy reviews the three main methods developed for the ultrastructural visualization
Immunocytochemistry and In Situ Hybridization in the Biomedical Sciences
Author | : Julian E. Beesley |
Publsiher | : Springer Science & Business Media |
Total Pages | : 277 |
Release | : 2012-12-06 |
Genre | : Science |
ISBN | : 9781461201397 |
Download Immunocytochemistry and In Situ Hybridization in the Biomedical Sciences Book in PDF, Epub and Kindle
Immunocytochemistry and in situ hybridization are widely used biomedical sciences. They are essential in medical diagnosis and in cell biology research. Affinity labeling is the central goal of the experimental strategy involving a series of techniques in a logical order; from the effects of specimen fixation, through specimen preparation to expose the antigen, to optimizing immunolabeling, to assessing the result and finally to safety considerations. Numerous examples of these techniques in biomedical sciences are included, as well as experimental assays and practical tips. This survey of methods will serve as an invaluable reference source in any laboratory setting (academic, industrial or clinical) involved in research in almost every branch of biology or medicine, as well as in pharmaceutical, biotechnological and clinical applications.
In Situ Hybridization
Author | : David G. Wilkinson |
Publsiher | : OUP Oxford |
Total Pages | : 243 |
Release | : 1998-11-26 |
Genre | : Science |
ISBN | : 9780191565700 |
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In situ hybridization is used to reveal the location of specific nucleic acids sequences on chromosomes or in tissues. Visualization of the location of genes on chromosomes or of specific mRNAs or viruses in tissues is crucial for understanding the organization, regulation, and function of genes. It is a therefore a core technique in all areas of biomedical research. In Situ Hybridization: A Practical Approach 2/e is the second edition of one of the most successful Practical Approach books, published in 1992. Since the first edition was published, a number of important technical advances have been made. The new edition has been thoroughly updated to contain protocols detailing the major techniques of in situ hybridization currently in use: in situ hybridization to mRNA with oligonucleotide and RNA probes (radiolabelled and hapten labelled); analysis using light and electron microscopes; whole mount in situ hybridization; double detection of RNAs, and RNA plus protein; and fluorescent in situ hybridization to detect chromosomal sequences. The protocols are complemented by advice on strategies for successful results, descriptions of the theoretical basis of in situ hybridization and important new developments in gene expression databases. The procedures described are widely applicable to many systems. The use of in situ hybridization in PCR is covered in a separate volume: Herrington and O'Leary (Eds) PCR 3 - PCR in situ hybridization: A Practical Approach (OUP, 1997). All the authors have extensive practical experience of establishing reliable techniques of in situ hybridization. This book will be useful to all researchers at all levels who use in situ hybridization.
SITU HYBRIDIZATION
Author | : LEITCH |
Publsiher | : Springer |
Total Pages | : 132 |
Release | : 1994-06-30 |
Genre | : Science |
ISBN | : MINN:31951D00905501H |
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This practical guide provides a comprehensive description of in situ hybridization, from background information to detailed and practical applications. In situ hybridization is a powerful link between cellular and molecular biology, and can be used to localize nucleic acid sequences.
PCR RT PCR in situ
Author | : Gerard Morel,Mireille Raccurt |
Publsiher | : CRC Press |
Total Pages | : 434 |
Release | : 2002-09-27 |
Genre | : Science |
ISBN | : 9781420042535 |
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Although the polymerase chain reaction has revolutionized genetic analysis by amplifying rare nucleic acid sequences, the in situ application is the only method that allows the localization of amplified signal within tissue structure. The applications of in situ polymerase chain reaction have greatly enhanced the field of investigation in many disc
Imaging of Nucleic Acids and Quantitation in Photonic Microscopy
Author | : Xavier Ronot,Yves Usson |
Publsiher | : CRC Press |
Total Pages | : 182 |
Release | : 2001-05-30 |
Genre | : Science |
ISBN | : 9781420041705 |
Download Imaging of Nucleic Acids and Quantitation in Photonic Microscopy Book in PDF, Epub and Kindle
Visualization of nuclear components has added tremendously to our understanding of the nucleus and its chemistry. The fluorescence approach is considered the present-day standard for comprehending nuclear organization. Imaging of Nucleic Acids and Quantitation in Photonic Microscopy provides new technologies in fluorescence microscopy and image cyt
In Situ Hybridization
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Author | : Gary Coulton,Jackie de Belleroche |
Publsiher | : Unknown |
Total Pages | : 150 |
Release | : 1998-11-01 |
Genre | : Science |
ISBN | : 3540571191 |
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Fluorescence In Situ Hybridization (FISH) is a powerful method that has become widely used in laboratories and has been applied to nearly every type of tissue, including human biopsy and autopsy samples, insects embedded in amber, mouse embryos, plant cell cultures, and naked dilated DNA, mRNA or chromosomes.