Insect Cell Cultures

A comprehensive reference work covering the key issues in insect cell cultures, this text includes 30 review papers on such topics as: cell lines (development, characterisation, physiology, cultivation and medium design); viruses (virus-cell interactions, replication, recombinant construction, infection kinetics, post-translational modification and passage effects); engineering (shear, bioreactors including perfusion, immobilisation, scale-up and modelling, downstream processing); applications; and economics and regulatory aspects.; This text should be useful for cell biologists, biochemists, molecular biologists, virologists, immunologists and other basic and applied disciplines related to cell culture engineering, both academic and industrial.

Consolidating and expanding current, fundamental notions of virology and animal cell cultivation, this practical reference examines the development of insect cell culture techniques for the production of recombinant proteins and insect pathogenic viruses.;Resolving on-the-job problems such as sparging cell damage and reduced infectivity cells, Insect Cell Culture Engineering: includes special introductory material as well as background information on insect pathogenic viruses, the molecular biology of baculoviruses and bioreactor design; offers advice on how to save time when deciding which insect cell line, bioreactor and medium to exploit; discusses the preparation of mathematical modelling in animal cell culture; addresses the concerns associated with insect cell immobilization and the use of serum-free culture media; provides insights into the protective effects of polymer additives and insect cell gene expression in pharmaceutical research; and analyzes process scale-up and reactor design.;Bridging the gap between laboratory research and pilot plant scale insect culture/baculovirus technology, Insect Cell Culture Engineering is designed as a reference for biochemical and bioprocess engineers, bioprocess technologists, biochemists, molecular and cell biologists, microbiologists, and upper-level undergraduate and graduate students in these disciplines.

Insect Cell Biotechnology provides a lucid, up-to-date description of recent major advances in the field. A number of significant topics are addressed, including the use and production of baculoviruses in insect cells, baculovirus specificity, bacterial toxin studies in cultured insect cells, scale-up operations required in the production of recombinant protein and insect viruses propagated in insect cells, growth and nervous system interactions, and the physiological and developmental capacities of cell lines. Transfection in Drosophila cells and a chapter on the theoretical and practical implications of stress produced by x-rays, ultraviolet light, chemicals, psoralens, and heat are discussed as well.

The third edition of this volume expands upon the previous two editions with new and up-to-date methods and protocols. Chapters include step-by-step procedures involved in quantifying cell growth, baculovirus infection and cell metabolism, methods to isolate new cell lines and develop your own serum-free medium, and routine maintenance and storage of insect cell lines and baculoviruses, small- and large-scale recombinant protein production with the BEVS in both insect and mammalian cell culture and in insect larvae, production and characterization of baculoviruses, green fluorescent protein, tubular reactors and RNAi, and baculovirus/insect cell system to study apoptosis and generating envelop-modified baculovirus for gene delivery into mammalian cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Baculovirus and Insect Cell Expression Protocols, Third Edition aims to not only aid the user in successfully completing the tasks described, but also stimulate the development of improved techniques and new applications of baculoviruses and insect cell culture.

A useful reference for those using or interested in cultured invertebrate cells, this two-volume text provides information about techniques and advances in invertebrate tissue culture. Cell lines for Insecta, Crustacea, Mollusca, and Nematoda are introduces along with their characterizations. Developments in insect biotechnology, including foreign protein production by insect cells infected with recombinant virus are described. Fundamental studies for introducing foreign genes into cultured insect cells is also presented. Wide information on studies -at cellular levels-on pathogens of insects, plants, and vertebrates is given.

The completion of the Human Genome Project and the rapid progress in cell bi- ogy and biochemical engineering, are major forces driving the steady increase of approved biotech products, especially biopharmaceuticals, in the market. Today mammalian cell products (“products from cells”), primarily monoclonals, cytokines, recombinant glycoproteins, and, increasingly, vaccines, dominate the biopharmaceutical industry. Moreover, a small number of products consisting of in vitro cultivated cells (“cells as product”) for regenerative medicine have also been introduced in the market. Their efficient production requires comprehensive knowledge of biological as well as biochemical mammalian cell culture fundamentals (e.g., cell characteristics and metabolism, cell line establishment, culture medium optimization) and related engineering principles (e.g., bioreactor design, process scale-up and optimization). In addition, new developments focusing on cell line development, animal-free c- ture media, disposables and the implications of changing processes (multi-purpo- facilities) have to be taken into account. While a number of excellent books treating the basic methods and applications of mammalian cell culture technology have been published, only little attention has been afforded to their engineering aspects. The aim of this book is to make a contribution to closing this gap; it particularly focuses on the interactions between biological and biochemical and engineering principles in processes derived from cell cultures. It is not intended to give a c- prehensive overview of the literature. This has been done extensively elsewhere.

The submersed cultivation of organisms in sterile containments or fermenters has become the standard manufacturing procedure, and will remain the gold standard for some time to come. This book thus addresses submersed cell culture and fermentation and its importance for the manufacturing industry. It goes beyond expression systems and integrally investigates all those factors relevant for manufacturing using suspension cultures. In so doing, the contributions cover all industrial cultivation methods in a comprehensive and comparative manner, with most of the authors coming from the industry itself. Depending on the maturity of the technology, the chapters address in turn the expression system, basic process design, key factors affecting process economics, plant and bioreactor design, and regulatory aspects.

This text aims to provide readers with a balanced cross-section of current developments within the research on invertebrate cell culture. Attention is focused on such topics as: the biochemistry and physiology of cultured invertebrate cells; aspects of virus infection; novel cultivation methods; assays of viruses affecting shrimp and insect cells; engineering of invertebrate cells for the production of baculovirus pesticides; application of microgravity to in vitro cell cultivation; and other aspects of biotechnology. The large body of information brings into focus the significant recent achievements in the laboratories of Africa, America, Europe and Asia.