Insect Viruses

There are over a million different species of insects, and individually they 8 outnumber humans by more than 10 to 1. Moreover, some insects live in close association with both plants and higher animals and naturally exchange viruses with them. It has even been speculated that viruses in general may have radiated through the plant and animal kingdoms from common insect origins. Be that as it may, since insects play pivotal roles in the biosphere, both to the benefit and detriment of mankind, they and the viruses that infect them are important subjects for study. Insects are infected by a diverse medley of viruses, and this volume focuses on those insect virus families that are found primarily or exclusively in insects. All major families of insect-selective viruses are covered except for the baculo viruses, which were described in a separate volume of The Viruses series. Included in this volume are the established families of insect viruses, the newly recognized ascovirus family, and the nudiviruses, which probably represent a separate family but currently remain unclassified. The coverage of arboviruses is limited to a single chapter that focuses on their potential utility as vectors and in insect control. Omitted for practical reasons are several individual insect viruses that have been described sporadically in the literature and probably represent novel virus families.

This volume contains seven chapters, based on papers presented at a Symposium on Insect Viruses, held in conjunction with the 67th Annual Meeting of the American Society for Microbiology in New York, N. Y. , on 30 April-4 May, 1967. The Symposium was organized to bring together outstanding workers interested in various areas of insect virology, and allow an opportunity for a discussion of problems, approaches, and methods that would lead to further progress in basic and applied research. One of the princi pal reasons for holding the Symposium at this time was the feeling that the divergent areas of research, up to now studied separately by entomologists, medical and public health workers, geneticists, and plant pathologists, would be brought together, crossing the artificial borders and finding new, exciting and inspiring vistas. Insect viruses provide a rare opportunity to get acquainted with the work and methods of investigators in such related and yet distant fields. Following the symposium, a decision was made to publish the papers in a single volume, extending the contents to provide a complete and scholarly review of each subject. Since viruses affecting insects have received little attention until recent years, it was felt that a fully documented presentation of diverse areas of insect virology merited publication. The invited authors, all recognized authorities in their respective fields, prepared their contri butions in such a way that each is a concise unit.

Baculoviruses are perhaps unique among viruses in the breadth of their biotechnological applications: these insect specific viruses are used not only for insect pest management purposes, but also as laboratory research tools for production of recombinant proteins and for protein display, and as potential vectors for human gene therapy. In addition to highlighting recent advances, this volume provides a comprehensive review of the biotechnological applications of these and other insect viruses in both the academic and private sectors.

This is an essential guidebook, providing a comprehensive overview of insect viruses and pest management. Part One of this volume explores the rationale behind the employment of insect pathogenic viruses in pest control and documents the assessment of biological activity, the ecology of baculoviruses, control strategies, virus production and formulation, and the conduct and recording of field control trials. Part Two comprises an authoritative global survey of current practice, R&D, and up-to-date technical studies of insect viruses and their application in pest management. This survey was compiled with the assistance of a panel of world-wide experts and will prove an invaluable and unique data source. Building on the key topics discussed in Part One, easy-to-follow, practical protocols are presented in Part Three, including detailed accounts of standard operating procedures for working with insects, isolation, propagation (in vivo and in vitro), purification, characterization and enumeration of viruses, suggestions for good laboratory layout and design, mass production methods, formulation and quality control. The importance of external environmental factors concerning virus survival and efficacy is also not forgotten, and in the final part the effects of solar radiation and the relationships between viruses and plant surfaces are discussed. Indispensable reading for all professionals and students interested in insect virology and pest control, this book is a comprehensive reference manual.

Baculoviruses are perhaps unique among viruses in the breadth of their biotechnological applications: these insect specific viruses are used not only for insect pest management purposes, but also as laboratory research tools for production of recombinant proteins and for protein display, and as potential vectors for human gene therapy. In addition to highlighting recent advances, this volume provides a comprehensive review of the biotechnological applications of these and other insect viruses in both the academic and private sectors.

Insects are a major group of arthropods and the most diverse group of animals on the earth, with over a million described species. In common with all other life forms, insects suffer from viruses that cripple and kill. Admittedly, insects transmit viruses that cause illness in humans, but the insect in such cases is usually unaffected. This book includes research on such viruses affecting humans. Also included in this book are the ways to recognize insect viruses and their use in pest control. Basic and applied research on insect virus proteins associated with the peroral infectivity is reviewed as well. Furthermore, RNAi has been applied to a number of studies involved in insect immunity. Thus, the effect of RNA interference on viral infections is also studied.

The various types of insect viruses and the nuclear polyhedroses; The polyhedroses; Cytoplasmic type; The granuloses; The noninclusion and miscellaneous virus deseases; Mode of replication of insect viruses; Latent viral infections; Tissue culture of insect viruses; Further aspects of the relationships between insects and viruses; Plant virus-insect vector relationships; Viruses and the biological control of insect pests.

Pathogens transmitted among humans, animals, or plants by insects and arthropod vectors have been responsible for significant morbidity and mortality throughout recorded history. Such vector-borne diseases â€" including malaria, dengue, yellow fever, and plague â€" together accounted for more human disease and death in the 17th through early 20th centuries than all other causes combined. Over the past three decades, previously controlled vector-borne diseases have resurged or reemerged in new geographic locations, and several newly identified pathogens and vectors have triggered disease outbreaks in plants and animals, including humans. Domestic and international capabilities to detect, identify, and effectively respond to vector-borne diseases are limited. Few vaccines have been developed against vector-borne pathogens. At the same time, drug resistance has developed in vector-borne pathogens while their vectors are increasingly resistant to insecticide controls. Furthermore, the ranks of scientists trained to conduct research in key fields including medical entomology, vector ecology, and tropical medicine have dwindled, threatening prospects for addressing vector-borne diseases now and in the future. In June 2007, as these circumstances became alarmingly apparent, the Forum on Microbial Threats hosted a workshop to explore the dynamic relationships among host, pathogen(s), vector(s), and ecosystems that characterize vector-borne diseases. Revisiting this topic in September 2014, the Forum organized a workshop to examine trends and patterns in the incidence and prevalence of vector-borne diseases in an increasingly interconnected and ecologically disturbed world, as well as recent developments to meet these dynamic threats. Participants examined the emergence and global movement of vector-borne diseases, research priorities for understanding their biology and ecology, and global preparedness for and progress toward their prevention, control, and mitigation. This report summarizes the presentations and discussions from the workshop.