Lab On A Chip

Responding to the need for an affordable, easy-to-read textbook that introduces microfluidics to undergraduate and postgraduate students, this concise book will provide a broad overview of the important theoretical and practical aspects of microfluidics and lab-on-a-chip, as well as its applications.

Labs on Chip: Principles, Design and Technology provides a complete reference for the complex field of labs on chip in biotechnology. Merging three main areas— fluid dynamics, monolithic micro- and nanotechnology, and out-of-equilibrium biochemistry—this text integrates coverage of technology issues with strong theoretical explanations of design techniques. Analyzing each subject from basic principles to relevant applications, this book: Describes the biochemical elements required to work on labs on chip Discusses fabrication, microfluidic, and electronic and optical detection techniques Addresses planar technologies, polymer microfabrication, and process scalability to huge volumes Presents a global view of current lab-on-chip research and development Devotes an entire chapter to labs on chip for genetics Summarizing in one source the different technical competencies required, Labs on Chip: Principles, Design and Technology offers valuable guidance for the lab-on-chip design decision-making process, while exploring essential elements of labs on chip useful both to the professional who wants to approach a new field and to the specialist who wants to gain a broader perspective.

HereOCOs a groundbreaking book that introduces and discusses the important aspects of lab-on-a-chip, including the practical techniques, circuits, microsystems, and key applications in the biomedical, biology, and life science fields. Moreover, this volume covers ongoing research in lab-on-a-chip integration and electric field imaging. Presented in a clear and logical manner, the book provides you with the fundamental underpinnings of lab-on-a-chip, presents practical results, and brings you up to date with state-of-the-art research in the field. This unique resource is supported with over 160 illustrations that clarify important topics throughout.

I TECHNOLOGIES -- Hydrogels and polymers as components of a lab on a chip -- Microreplication technologies for polymer-based æTAS applications -- Silicon and glass micromachining for æTAS -- Surface chemistry in polymer microfluidic systems -- Plastic microfluidic devices: electrokinetic manipulations, life science applications, and production technologies -- II METHODS -- Transverse diffusion in microfluidic systems -- Nanoliter & picoliter liquid handling -- Micro sequential injection system for monitoring of metabolites extruded by cultured cells -- III CELL- & BEAD-BASED SYSTEMS -- Handling of beads in microfluidic devices for biotech applications -- Particles and molecules handling in micro channels -- Cell counting and cell sizing in microstructures -- IV APPLICATIONS -- Microfabricated capillary array electrophoresis: -- implementation and applications -- Microfluidic systems for analysis of the proteome with mass spectrometry -- Interfacing æTAS to matrix assisted laser desorpt ...

The microfluidic lab-on-a-chip allows scientists to conduct chemical and biochemical analysis in a miniaturized format so small that properties and effects are successfully enhanced, and processes seamlessly integrated. This microscale advantage translates into greater sensitivity, more accurate results, and better information. Microfluidic

The necessity of on-site, fast, sensitive, and cheap complex laboratory analysis, associated with the advances in the microfabrication technologies and the microfluidics, made it possible for the creation of the innovative device lab-on-a-chip (LOC), by which we would be able to scale a single or multiple laboratory processes down to a chip format. The present book is dedicated to the LOC devices from two points of view: LOC fabrication and LOC application.

Microfluidic biochips have gained prominence due to their versatile applications to biochemistry and health-care domains such as point-of-care clinical diagnosis of tropical and cardiovascular diseases, cancer, diabetes, toxicity analysis, and for the mitigation of the global HIV crisis, among others. Microfluidic Lab-on-Chips (LoCs) offer a convenient platform for emulating various fluidic operations in an automated fashion. However, because of the inherent uncertainty of fluidic operations, the outcome of biochemical experiments performed on-chip can be erroneous even if the chip is tested a priori and deemed to be defect-free. This book focuses on the issues encountered in reliable sample preparation with digital microfluidic biochips (DMFBs), particularly in an error-prone environment. It presents state-of-the-art error management techniques and underlying algorithmic challenges along with their comparative discussions. Describes a comprehensive framework for designing a robust and error-tolerant biomedical system which will help in migrating from cumbersome medical laboratory tasks to small-sized LOC-based systems Presents a comparative study on current error-tolerant strategies for robust sample preparation using DMFBs and reports on efficient algorithms for error-tolerant sample dilution using these devices Illustrates how algorithmic engineering, cyber-physical tools, and software techniques are helpful in implementing fault tolerance Covers the challenges associated with design automation for biochemical sample preparation Teaches how to implement biochemical protocols using software-controlled microfluidic biochips Interdisciplinary in its coverage, this reference is written for practitioners and researchers in biochemical, biomedical, electrical, computer, and mechanical engineering, especially those involved in LOC or bio-MEMS design.

Multidisciplinary Microfluidic and Nanofluidic Lab-on-a-Chip: Principles and Applications provides chemists, biophysicists, engineers, life scientists, biotechnologists, and pharmaceutical scientists with the principles behind the design, manufacture, and testing of life sciences microfluidic systems. This book serves as a reference for technologies and applications in multidisciplinary areas, with an emphasis on quickly developing or new emerging areas, including digital microfluidics, nanofluidics, papers-based microfluidics, and cell biology. The book offers practical guidance on how to design, analyze, fabricate, and test microfluidic devices and systems for a wide variety of applications including separations, disease detection, cellular analysis, DNA analysis, proteomics, and drug delivery. Calculations, solved problems, data tables, and design rules are provided to help researchers understand microfluidic basic theory and principles and apply this knowledge to their own unique designs. Recent advances in microfluidics and microsystems for life sciences are impacting chemistry, biophysics, molecular, cell biology, and medicine for applications that include DNA analysis, drug discovery, disease research, and biofluid and environmental monitoring. Provides calculations, solved problems, data tables and design rules to help understand microfluidic basic theory and principles Gives an applied understanding of the principles behind the design, manufacture, and testing of microfluidic systems Emphasizes on quickly developing and emerging areas, including digital microfluidics, nanofluidics, papers-based microfluidics, and cell biology