Legume Genetics And Biology

Legumes have played an important part as human food and animal feed in cropping systems since the dawn of agriculture. The legume family is arguably one of the most abundantly domesticated crop plant families. Their ability to symbiotically fix nitrogen and improve soil fertility has been rewarded since antiquity and makes them a key protein source. Pea was the original model organism used in Mendel´s discovery of the laws of inheritance, making it the foundation of modern plant genetics. This book based on Special Issue provides up-to-date information on legume biology, genetic advances, and the legacy of Mendel.

Legumes have played an important part as human food and animal feed in cropping systems since the dawn of agriculture. The legume family is arguably one of the most abundantly domesticated crop plant families. Their ability to symbiotically fix nitrogen and improve soil fertility has been rewarded since antiquity and makes them a key protein source. Pea was the original model organism used in Mendel ́s discovery of the laws of inheritance, making it the foundation of modern plant genetics. This book based on Special Issue provides up-to-date information on legume biology, genetic advances, and the legacy of Mendel.

Featuring current resources used to discover new legume family genes and to understand genes and their interactions, Legume Genomics: Methods and Protocols provides techniques from expert researchers to study these plants that are so vitally important for food, feed, human nutrition, bioenergy, and industrial purposes. This detailed volume covers genome characterization and analysis, transcriptome analysis and miRNA identification/analysis, forward and reverse genetics, molecular markers, as well as transformation strategies used to investigate gene function and many other topics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and useful, Legume Genomics: Methods and Protocols aims to serve plant molecular biologists, molecular breeders, plant physiologists and biochemists, developmental biologists, and those interested in plant-microbe interactions.

Food legumes are important constituents of the human diet and animal feed where they are crucial to a balanced diet, supplying high quality proteins. These crops also play an important role in low-input agricultural production systems by fixing atmospheric nitrogen. Despite systematic and continuous breeding efforts through conventional methods, substantial genetic gains have not been achieved. With the rise in demand for food legumes/pulses and increased market value of these crops, research has focused on increasing production and improving the quality of pulses for both edible and industria.

Grain legumes, including common-bean, chickpea, pigeonpea, pea, cowpea, lentil and others, form important constituents of global diets, both vegetarian and non-vegetarian. Despite this significant role, global production has increased only marginally in the past 50 years. The slow production growth, along with a rising human population and improved buying capacity has substantially reduced the per capita availability of food legumes. Changes in environmental climate have also had significant impact on production, creating a need to identify stable donors among genetic resources for environmentally robust genes and designing crops resilient to climate change. Genetic and Genomic Resources of Grain Legume Improvement is the first book to bring together the latest resources in plant genetics and genomics to facilitate the identification of specific germplasm, trait mapping and allele mining to more effectively develop biotic and abiotic-stress-resistant grains. This book will be an invaluable resource for researchers, crop biologists and students working with crop development. Explores origin, distribution and diversity of grain legumes Presents information on germplasm collection, evaluation and maintenance Offers insight into pre-breeding/germplasm enhancement efforts Integrates genomic and genetic resources in crop improvement Internationally contributed work

This volume looks at the latest techniques used by researchers to help them understand the biology of various cellular processes and agronomic traits, and come up with better strategies to improve legume crops. The chapters in this book cover topics such as legume genomic resources; legume pangenome and organelle genome construction; transcriptome analysis; DNA methylation analysis; double-digest restriction site-associated DNA sequencing; target enrichment sequencing via probe capture; genomic selection and transformation methods; prediction of long non-coding RNAs and secondary structures; genome-wide mining of disease resistance gene analogs; genome editing, and bioactive compound and phosphoproteome analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and informative, Legume Genomics: Methods and Protocols is a useful reference for genomicists, molecular breeders, plant molecular biologists, biotechnologists, computational biologists, and developmental biologists. This book is also an excellent resource for any novice and expert researcher involved in various molecular aspects of legume biology or general plant studies.

Legumes in the Omic Era provides a timely review of recent advances in legume genomics research and application. In this post-genomic era enormous amount of biological information is available which could be of huge potential use for crop improvement applications. This aspect of genomics assisted plant breeding is focused throughout the book for all the important grain legume crops. Role of functional genomics and importance of bioinformatics tools in present day genomics and molecular breeding research is also discussed in detail. Use of molecular tools for nutritional fortification of grain legume is briefly presented. A chapter also been contributed on fungal disease resistance to elucidate potential application of genomic tools in molecular breeding of grain legume species. The book contains fifteen chapters contributed by 50 scientists from different countries who are actively involved in analyzing and improving particular legume genome. This book will serve as reference resource to legumes researchers for use of genome information in improvement of major legume crops. Dr Sanjeev Gupta is Principal Scientist/Project Coordinator-All India Coordinated Research Project on Vigna Crops at Indian Institute of Pulses Research (IIPR), Kanpur. He has more than two decades of research experience in grain legume breeding and developed a number of high yielding cultivars in grain legumes. He is authored numerous research papers published in peer-reviewed journals and edited several books in plant breeding aspects. He was the Organizing Secretary of the International Grain Legume Conference, 2009 held in the Indian Institute of Pulses Research, Kanpur, India. He has travelled across the continents to present his research several times. He is recipient of several awards for his research and literary contributions Dr. Nagasamy Nadarajan is the Director of the Indian Institute of Pulses Research (IIPR), Kanpur. He has more than three decades of teaching and research experience and developed more than fifteen legume and cereal cultivars. He has to his credits more than 200 peer-reviewed research publications. He has guided several graduate students for Masters and Doctoral degrees in food legume breeding and genetics research. He has authored a book in biometrics which is one of the most popular books among the agriculture graduate students in India. He is the recipient of three international and six national awards and honours for his outstanding contributions Mr. Debjyoti Sen Gupta is the ICAR International Fellow and Ph.D. candidate at North Dakota State University (NDSU), Fargo, USA. Recently, he visited Department of Crop and Soil Sciences, Washington State University, Pullman, USA for high throughput genotyping work. Before joining at NDSU he was serving as the Scientist in the Indian Institute of Pulses Research (IIPR). He has authored several research articles, review articles and book chapters in the peer-reviewed journals and books from reputed publishers like Springer, CABI etc. He is recipient of several fellowships like CSIR-JRF, New Delhi; ICAR-JRF, New Delhi throughout his graduate study programs.

This edited volume provides state-of–the-art overview of abiotic stress responses and tolerance mechanisms of different legume crops viz., chickpea, mung bean, lentil, black gram, cowpea, cluster bean, soybean and groundnut. Legumes play an important role in human nutrition and soil health through fixation of nitrogen. Legume production and productivity are vulnerable to different abiotic stresses. A proper understanding about the physiological and molecular basis of the legume crops is essential for genetic improvement of abiotic stress tolerance. This book consists of 15 chapters covering physiological and biochemical basis, molecular physiology, molecular breeding, genetics, genomics, transgenics, epigenetics of drought, saline, high temperature and nutrient deficiency stresses, and the role of microRNAs in abiotic stress tolerance. This volume offers new perspectives in legume crop abiotic stress management, and is useful for various stakeholders, including post graduates students, scientists, environmentalists and policymakers.