Measuring Calcium And Calmodulin Inside And Outside Cells

The study of signal transduction mechanisms has become one of the most important branches of biomedical science, indispensable to understanding the normal actions of hormones, neurotransmitters and other extracellular signaling molecules as well as many pathophysiological processes, including inflammation and cancer. In this volume, the various techniques for measuring Ca2+ in the cytosol, in the various organelles, and in the immediate surroundings of individual cells are described in practical detail by experts. Also included is a chapter on tracking calmodulin inside cells using fluorescence technology. A Springer Lab Manual

Calcium and Cell Function, Volume 7 covers the signal transduction across the cell membrane. The book discusses phosphoinositides and calcium signaling; calmodulin-stimulated adenylate cyclases; and calcium/calmodulin-dependent protein kinases. The text also describes the regulation of gene expression by calcium; the dynamics of intracellular calcium concentration during mitosis; and the methods for the measurement of intracellular ionized calcium in mammalian cells. Pharmacologists, physiologists, and people involved in the study of calcium and cell function will find the book invaluable.

Every cell of the body is dependent on calcium to function. Calcium is found in teeth and bones, and calcium signalling is necessary for the movement of muscles and for the action of the heart and the intestines as well as blood coagulation. Calcium in Living Cells will update classic techniques in detecting microscopic levels of calcium ions (Ca2+) in living cells, as well as address new techniques in the field of calcium detection and calcium signaling. Such detection and measurement of intracellular calcium is important to researchers studying the heart, musculoskeletal, gastrointestinal, and immune systems, whose findings will aid in the advancement of drug and genomic therapies to treat heart, gastrointestinal, autoimmune, and infectious diseases. Gives researchers much needed information on how to study calcium in live cells, which is becoming increasingly important in heart, musculoskeletal, and immune system research Provides an overview of the latest methods--fluorescence resonance energy transfer (FRET), for example-- that are new to the field Allows understanding of how calcium plays a role in intracellular function at the cellular level, which has proved important in Alzheimer's research, heart disease, and areas of musculoskeletal research Updated chapters reflect advancements in the classic techniques used'preparing calcium buffers, vibrating the Ca2+ Electrode and confocal imaging

Calcium and Cell Function, Volume 7 covers the signal transduction across the cell membrane. The book discusses phosphoinositides and calcium signaling; calmodulin-stimulated adenylate cyclases; and calcium/calmodulin-dependent protein kinases. The text also describes the regulation of gene expression by calcium; the dynamics of intracellular calcium concentration during mitosis; and the methods for the measurement of intracellular ionized calcium in mammalian cells. Pharmacologists, physiologists, and people involved in the study of calcium and cell function will find the book invaluable.

The endothelium, a monolayer of endothelial cells, constitutes the inner cellular lining of the blood vessels (arteries, veins and capillaries) and the lymphatic system, and therefore is in direct contact with the blood/lymph and the circulating cells. The endothelium is a major player in the control of blood fluidity, platelet aggregation and vascular tone, a major actor in the regulation of immunology, inflammation and angiogenesis, and an important metabolizing and an endocrine organ. Endothelial cells controls vascular tone, and thereby blood flow, by synthesizing and releasing relaxing and contracting factors such as nitric oxide, metabolites of arachidonic acid via the cyclooxygenases, lipoxygenases and cytochrome P450 pathways, various peptides (endothelin, urotensin, CNP, adrenomedullin, etc.), adenosine, purines, reactive oxygen species and so on. Additionally, endothelial ectoenzymes are required steps in the generation of vasoactive hormones such as angiotensin II. An endothelial dysfunction linked to an imbalance in the synthesis and/or the release of these various endothelial factors may explain the initiation of cardiovascular pathologies (from hypertension to atherosclerosis) or their development and perpetuation. Table of Contents: Introduction / Multiple Functions of the Endothelial Cells / Calcium Signaling in Vascular Cells and Cell-to-Cell Communications / Endothelium-Dependent Regulation of Vascular Tone / Conclusion / References

T cells play a vital role mediating adaptive immunity, a specific acquired resistance to an infectious agent produced by the introduction of an antigen. There are a variety of T cell types with different functions. They are called T cells, because they are derived from the thymus gland. This volume discusses how T cells are regulated through the operation of signaling mechanisms. Topics covered include positive and negative selection, early events in T cell receptor engagement, and various T cell subsets.

A comprehensive compendium of scholarly contributions relating to bacterial virulence gene regulation. • Provides insights into global control and the switch between distinct infectious states (e.g., acute vs. chronic). • Considers key issues about the mechanisms of gene regulation relating to: surface factors, exported toxins and export mechanisms. • Reflects on how the regulation of intracellular lifestyles and the response to stress can ultimately have an impact on the outcome of an infection. • Highlights and examines some emerging regulatory mechanisms of special significance. • Serves as an ideal compendium of valuable topics for students, researchers and faculty with interests in how the mechanisms of gene regulation ultimately affect the outcome of an array of bacterial infectious diseases.

Store-operated calcium channels are found in most animal cells and regulate many cellular functions including cell division, growth, differentiation, and cell death. This volume provides a concise and informative overview of the principles of store-operated calcium entry and the key developments in the field from researchers who have led these advances. The overall goal of the volume is to provide interested students and investigators with sufficient information to enable a broad understanding of the progress and current excitement in the field. The volume contains a wealth of information that even experienced investigators in the field will find useful. The volume provides a comprehensive overview of the mechanisms and functions of store-operated calcium channels. Contributors are authoritative researchers who have produced important advances in the field. The volume is well-illustrated with cartoons and data to facilitate easy comprehension of the subject.