Microrna Detection And Pathological Functions

This book summarizes microRNA (miRNA) biology in a variety of pathological processes, emphasizing the significant potential applications of miRNA in diagnostics and prognostics, as well as novel drug targets. The conventional techniques used for miRNA detection including standard PCR, Northern blotting, microarray and clone methods are addressed. Recent emerging strategies in miRNA detection and quantification with superior flexibility and adaptability, such as novel molecular biological techniques and locked nucleic acid (LNA) modified probes, as well as nanotechnology-based approaches, are also included. The book also highlights the latest advances in clinical-related miRNA detection methods in living cells, circulating blood and tissue, such as in situ hybridization (ISH) and molecular imaging techniques, which are useful to elucidate the biogenesis and biological function of miRNAs in vivo. Finally, the respective advantages and drawbacks of various detection techniques in this fast-moving field are discussed, along with the challenges and promising new directions. This book offers a valuable resource for analytical chemists, biologists and physicians involved in miRNA research. Dr. Xueji Zhang and Dr. Haifeng Dong are Professors at the School of Chemistry & Biological Engineering, University of Science & Technology Beijing (USTB), China. Dr. Yaping Tian is a Professor at the Department of Clinical Biochemistry, Chinese PLA General Hospital and Military Medical School, China.

This volume contains 17 short review articles classified into 3 parts. Part I consists of 7 articles dealing with basic aspects of contractile mechanism in skeletal and smooth muscle cells and also function of melanocytes having many properties common to those of smooth muscles. Part II and Part III contain articles dealing with pathological aspects of cardiac and smooth muscle cell functions, and dealing with factors influencing structure and function of cardiac and smooth muscle cells and tissues. The Editor believes that these articles are stimulating and informative for readers interested in basic, pathological and clinical aspects of muscle cells and tissues.

The field of cancer diagnosis, prognosis, and treatment is constantly advancing. From novel biomarkers to cutting-edge imaging solutions, changing chemotherapy protocols and novel immune-targeting agents, medical teams develop and test new ways to manage this ever-growing threat to the modern age. Imaging has been a reliable method for initial diagnosis and later surveillance of premalignant and cancerous lesions of the digestive tract. This book project aims to characterize the main diagnostic procedures and novel medical and surgical treatments, as well as provide an updated view on current guidelines, premalignant lesions management, and minimally invasive curative techniques.

This book includes updated information about microRNA regulation, for example, in the fields of circular RNAs, multiomics analysis, biomarkers and oncogenes. The variety of topics included in this book reaffirms the extent to which microRNA regulation affects biological processes. Although microRNAs are not translated to proteins, their importance for biological processes is not less than proteins. An understanding of their roles in various biological processes is critical to understanding gene function in these biological processes. Although non-coding RNAs other than microRNAs have recently come under investigation, microRNA still remains the front runner as the subject of genetic and biological studies. In reading the collection of papers, readers can grasp the most updated information regarding microRNA regulation, which will continue to be an important topic in genetics and biology.

This book is addressed to physicians and researchers working in the ever-expanding research and practice fields of transplantation medicine. Its purpose is to present the transplantation community with a collection of works written by prominent experts in a variety of transplant-related fields, encompassing the most recent scientific and practical developments and accomplishments in the highly specialized segment of transplantation medicine, such as perioperative care for organ transplant candidates and recipients.

microRNAs (miRNAs) are small non-coding RNAs that regulate various biological phenomena, such as development and homeostasis. The dysregulation of miRNA leads to disease progression, particularly of cancer. In Circulating MicroRNAs: Methods and Protocols, expert researchers in the field detail recent advances in the isolation, purification and analysis of circulating miRNAs from a variety of sources for research. The book is divided into three main topics. The first section involves the study of secretory miRNAs in cell-cell communication, and the second, the study of circulating miRNAs in body fluids. The last describes the novel techniques used to study circulating miRNAs. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Circulating MicroRNAs: Methods and Protocols seeks to aid scientists in dealing with the recent advances of RNAi technology from the bench to the bedside.

In recent years, advanced molecular techniques in diagnostic microbiology have been revolutionizing the practice of clinical microbiology in the hospital setting. Molecular diagnostic testing in general and nucleic acid-based amplification methods in particular have been heralded as diagnostic tools for the new millennium. This third edition covers not only the most recent updates and advances, but details newly invented omic techniques, such as next generation sequencing. It is divided into two distinct volumes, with Volume 1 describing the techniques, and Volume 2 addressing their applications in the field. In addition, both volumes focus more so on the clinical relevance of the test results generated by these techniques than previous editions.

Computational Non-coding RNA Biology is a resource for the computation of non-coding RNAs. The book covers computational methods for the identification and quantification of non-coding RNAs, including miRNAs, tasiRNAs, phasiRNAs, lariat originated circRNAs and back-spliced circRNAs, the identification of miRNA/siRNA targets, and the identification of mutations and editing sites in miRNAs. The book introduces basic ideas of computational methods, along with their detailed computational steps, a critical component in the development of high throughput sequencing technologies for identifying different classes of non-coding RNAs and predicting the possible functions of these molecules. Finding, quantifying, and visualizing non-coding RNAs from high throughput sequencing datasets at high volume is complex. Therefore, it is usually possible for biologists to complete all of the necessary steps for analysis. Presents a comprehensive resource of computational methods for the identification and quantification of non-coding RNAs Introduces 23 practical computational pipelines for various topics of non-coding RNAs Provides a guide to assist biologists and other researchers dealing with complex datasets Introduces basic computational methods and provides guidelines for their replication by researchers Offers a solution to researchers approaching large and complex sequencing datasets