Micrornas As Tools In Biopharmaceutical Production
Download Micrornas As Tools In Biopharmaceutical Production full books in PDF, epub, and Kindle. Read online free Micrornas As Tools In Biopharmaceutical Production ebook anywhere anytime directly on your device. Fast Download speed and no annoying ads. We cannot guarantee that every ebooks is available!
MicroRNAs as Tools in Biopharmaceutical Production
Author | : Niall Barron |
Publsiher | : Springer Science & Business Media |
Total Pages | : 126 |
Release | : 2012-11-15 |
Genre | : Medical |
ISBN | : 9789400751286 |
Download MicroRNAs as Tools in Biopharmaceutical Production Book in PDF, Epub and Kindle
Focused manuscript on the potential use/role of miRNAs in bioprocessing, specifically the production of complex proteins in mammalian cells. With that in mind I propose a draft list of topics/chapters along the following lines: Intro on CHO/bioprocessing/engineering challenges to set scene, Genomic organization, biogenesis and mode of action, Identifying miRNA targets: Computational prediction, transcriptomics, proteomices, UTR analysis, etc., miRNA expression in Chinese Hamster Ovary cells, miRNAs as engineering targets: pathway manipulation to impact bioprocess phenotypes, miRNAs as biomarkers, Detection methods: Northern, PCR, hybridization arrays, Next Gen Seq, Manipulation of expression in cultured cells: Transient/stable disregulation, Knockout.
Cell Culture Engineering and Technology
Author | : Ralf Pörtner |
Publsiher | : Springer Nature |
Total Pages | : 552 |
Release | : 2022-02-20 |
Genre | : Medical |
ISBN | : 9783030798710 |
Download Cell Culture Engineering and Technology Book in PDF, Epub and Kindle
This contributed volume is dedicated towards the progress achieved within the last years in all areas of Cell Culture Engineering and Technology. It comprises contributions of active researchers in the field of cell culture development for the production of recombinant proteins, cell line development, cell therapy and gene therapy, with consideration of media development, process scale-up, reactor design, monitoring and control and model-assisted strategies for process design. The knowledge and expertise of the authors cover disciplines like cell biology, engineering, biotechnology and biomedical sciences. This book is conceived for graduate students, postdoctoral fellows and researchers interested in the latest developments in Cell Engineering.
Biopharmaceutical Processing
Author | : Gunter Jagschies,Eva Lindskog,Karol Lacki,Parrish M. Galliher |
Publsiher | : Elsevier |
Total Pages | : 1308 |
Release | : 2018-01-18 |
Genre | : Technology & Engineering |
ISBN | : 9780128125526 |
Download Biopharmaceutical Processing Book in PDF, Epub and Kindle
Biopharmaceutical Processing: Development, Design, and Implementation of Manufacturing Processes covers bioprocessing from cell line development to bulk drug substances. The methods and strategies described are essential learning for every scientist, engineer or manager in the biopharmaceutical and vaccines industry. The integrity of the bioprocess ultimately determines the quality of the product in the biotherapeutics arena, and this book covers every stage including all technologies related to downstream purification and upstream processing fields. Economic considerations are included throughout, with recommendations for lowering costs and improving efficiencies. Designed for quick reference and easy accessibility of facts, calculations and guidelines, this book is an essential tool for industrial scientists and managers in the biopharmaceutical industry. Offers a comprehensive, go-to reference for daily work decisions Covers both upstream and downstream processes Includes case studies that emphasize financial outcomes Presents summaries, decision grids, graphs and overviews for quick reference
Plant MicroRNAs
Author | : Stefan de Folter |
Publsiher | : Humana Press |
Total Pages | : 363 |
Release | : 2019-01-31 |
Genre | : Science |
ISBN | : 1493990411 |
Download Plant MicroRNAs Book in PDF, Epub and Kindle
This detailed volume provides a collection of protocols for the study of miRNA functions in plants. Beginning with coverage of miRNA function, biogenesis, activity, and evolution in plants, the book continues by guiding readers through methods on the identification and detection of plant miRNAs, bioinformatic analyses, and strategies for functional analyses of miRNAs. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Plant MicroRNAs: Method and Protocols aims to ensure successful results in the further study of this vital area of plant science.
Protein Therapeutics 2 Volume Set
Author | : Tristan Vaughan,Jane Osbourn,Bahija Jallal |
Publsiher | : John Wiley & Sons |
Total Pages | : 762 |
Release | : 2017-12-04 |
Genre | : Medical |
ISBN | : 9783527340866 |
Download Protein Therapeutics 2 Volume Set Book in PDF, Epub and Kindle
In this practice-oriented two volume handbook, professionals from some of the largest biopharmaceutical companies and top academic researchers address the key concepts and challenges in the development of protein pharmaceuticals for medicinal chemists and drug developers of all trades. Following an introduction tracing the rapid development of the protein therapeutics market over the last decade, all currently used therapeutic protein scaffolds are surveyed, from human and non-human antibodies to antibody mimetics, bispecific antibodies and antibody-drug conjugates. This ready reference then goes on to review other key aspects such as pharmacokinetics, safety and immunogenicity, manufacture, formulation and delivery. The handbook then takes a look at current key clinical applications for protein therapeutics, from respiratory and inflammation to oncology and immune-oncology, infectious diseases and rescue therapy. Finally, several exciting prospects for the future of protein therapeutics are highlighted and discussed.
MicroRNAs
Author | : Krishnarao Appasani |
Publsiher | : Cambridge University Press |
Total Pages | : 580 |
Release | : 2009-08-20 |
Genre | : Science |
ISBN | : 0521118557 |
Download MicroRNAs Book in PDF, Epub and Kindle
MicroRNAs (miRNAs) are RNA molecules, conserved by evolution, that regulate gene expressions and their recent discovery is revolutionising both basic biomedical research and drug discovery. Expression levels of MiRNAs have been found to vary between tissues and with developmental stages and hence evaluation of the global expression of miRNAs potentially provides opportunities to identify regulatory points for many different biological processes. This wide-ranging reference work, written by leading experts from both academia and industry, will be an invaluable resource for all those wishing to use miRNA techniques in their own research, from graduate students, post-docs and researchers in academia to those working in R&D in biotechnology and pharmaceutical companies who need to understand this emerging technology. From the discovery of miRNAs and their functions to their detection and role in disease biology, this volume uniquely integrates the basic science with industry application towards drug validation, diagnostic and therapeutic development. Forewords by: Sidney Altman, Yale University, Winner of the Nobel Prize in Chemistry, 1989 and Victor R. Ambros, Dartmouth Medical School, Co-discoverer of MicroRNAs
Identification of novel modulators towards high cell density and high producing Chinese hamster ovary suspension cell cultures as well as their application in biopharmaceutical protein production
Author | : Beat Thalmann |
Publsiher | : Logos Verlag Berlin GmbH |
Total Pages | : 221 |
Release | : 2015-08-28 |
Genre | : Animal cell biotechnology |
ISBN | : 9783832540463 |
Download Identification of novel modulators towards high cell density and high producing Chinese hamster ovary suspension cell cultures as well as their application in biopharmaceutical protein production Book in PDF, Epub and Kindle
Amongst the mammalian producer cell lines, the Chinese hamster ovary (CHO) cell lines are of predominant importance in biopharmaceutical production. Thus, novel factors increasing overall productivity are sought and bear the potential to reduce the unit costs of a production process. Furthermore, the current patent situation for several therapeutic proteins demands innovative tools to at least maintain or preferentially increase the cost-effectiveness of their production processes. In this thesis, hitherto unknown factors were revealed by next generation sequencing of chemically mutated and selected CHO-K1 suspension cell lines. Two factors were proven to improve CHO-based production processes: cgrSnord78 and cgrTtc36. The Cricetulus griseus Ttc36 increases the integral as well as the maximal viable cell density and abolishes the cell-cell aggregation whilst cgrSnord78 improves the specific as well as volumetric productivity without significant impact on cell growth. Based on the present results and discussion, foundations for future research on these functionally unrevealed factors are laid. Hence, this work represents the first step towards the application of the genuine biomolecules cgrTtc36 and cgrSnord78 in biopharmaceutical protein production.
Genetic Engineering Biotechnology News
Author | : Anonim |
Publsiher | : Unknown |
Total Pages | : 546 |
Release | : 2009 |
Genre | : Biotechnology |
ISBN | : PSU:000059773535 |
Download Genetic Engineering Biotechnology News Book in PDF, Epub and Kindle