Microsatellite Development And Application In Pigeonpea Cajanus Cajan L Millspaugh

Solanum quitoense (Lulo) from the northern Andes is a fruit with an attractive taste. It has a high export potential but its production is hampered by insufficient pollination. Studies on the reproductive system of Lulo revealed that, due to their poricidal anthers, the flowers need pollinators for self- and cross-pollination. Pollination by native bumblebees significantly increases fruit set and quality. Experiments show that colonies of Bombus atratus can be reared in captivity and used in Lulo fields for providing increased pollination services. Modeling based on data on colony development reveal larva/workers ratio and the supply of pollen as main parameters for improving the rearing method.

Coffea arabica, one of the economically most important crops worldwide, occurs naturally in the undergrowth of montane rainforests of Ethiopia. The study provides the first detailed ecophysiological investigations of wild coffee populations. It demonstrates the inter- and intra-regional variability in phenotypic and hydrological characteristics of wild coffee. The results reveal very different strategies of wild coffee seedlings for coping with drought stress. The ecophysiological diversity shows the importance of Ethiopian wild coffee populations as gene pools for future breeding programs, and underlines the need for an in-situ conservation strategy. The study includes recommendations for coffee forest management and the use of wild arabica coffee in Ethiopia.

This book emphasizes on cutting-edge next-generation smart plant breeding approaches for maximizing the use of genomic resources generated by high-throughput genomics in the post-genomic era. Through this book the readers would learn about the recent development in the genomic approaches such as genotype by sequencing (GBS) for genomic analysis (SNPs, Single Nucleotide Polymorphism), whole-genome re-sequencing (WGRS) and RNAseq for transcriptomic analysis (DEGs, Differentially Expressed Genes). To maximize the genetic gains in the cereal/food crops, the book covers topics on transgenic breeding, genome editing, high-throughput phenotyping, reliable/precision phenotyping and genomic information-based analysis. In the era of climate change and the ever-increasing population, food security and nutritional security are the primary concern of plant breeders, growers, and policymakers to address the UN’s sustainable development goals. Chapters of this book cohere around these goals and covers techniques such as (QTL mapping, association studies, candidate gene identification), omics, RNAi [through micro RNA (miRNA), small interfering RNA (siRNA) and artificial micro RNA (amiRNA)]. It also covers other genomic techniques like antisense technology, genome editing (CRISPR/cas9, base editing) and epigenomics that assist the crop improvement programmes to fulfil the UNs sustainable development goals. It explores the influence of rapidly available sequencing data assisting in the next generation breeding programmes. This volume is a productive resource for the students, researchers, scientists, teachers, public and private sector stakeholders involved in the genetic enhancement of cereal crops.

The exploitation of biodiversity is essential to select resilient genotypes for sustainable cropping systems as one of the main challenges for plant breeding. Mapping traits of agronomic interest in specific genomic regions appears as another pivotal effort for the future development of novel cultivars. For this purpose, there is evidence that MAGIC and other exotic populations will play a major role in the coming years in allowing for impressive gains in plant breeding for developing new generations of improved cultivars. This Special Issue focused on the application of advanced technologies devoted to crop improvement and exploit the available biodiversity in crops. In detail, next-generation sequencing (NGS) technologies supported the development of high-density genotyping arrays for different plants included in this issue.