Molecular Genetics Of Photosynthesis

Photosynthesis is one of the most active areas of plant science research. Advances in molecular biology technology have profoundly affected the work in this area and have opened up the possibility of manipulating the genes involved in photosynthesis. The field is expanding, and this book provides a compilation of authoritative reviews covering a variety of important issues.

Molecular biology, particularly molecular genetics, is among the newest and most powerful approach in modern photosynthesis research. Development of molecular biology techniques has provided new methods to solve old problems in many biological disciplines. Molecular biology has its greatest potential for contribution when applied in combination with other disciplines, to focus not just on genes and molecules, but on the complex interaction between them and the biochemical pathways in the whole organism. Photosynthesis is surely the best studied research area in plant biology, making this field the foremost candidate for successfully employing molecular genetic techniques. Already, the success of molecular biology in photosynthesis has been nothing short of spectacular. Work performed over the last few years, much of which is sum marized in this volume, stands in evidence. Techniques such as site-specific mutagenesis have helped us in examining the roles of individual protein domains in the function of multiunit complexes such as the enzyme ribulose-l ,5-bisphos phate carboxylase/oxygenase (RUBISCO) and the oxygen evolving photo system (the photosystem II). The techniques of molecular biology have been very important in advancing the state of knowledge of the reaction center from the photosynthetic bacteria whose structure has been elegantly deduced by H. Michel and 1. Deisenhofer from the X-ray studies of its crystals.

Provides a thorough overview of current research with the green alga Chlamydomonas on chloroplast and mitochondrial biogenesis and function, with an emphasis on the assembly and structure-function relationships of the constituents of the photosynthetic apparatus. Contributions emphasize the multidisciplinary nature of current research in photosynthesis, combining molecular genetics, biochemical, biophysical, and physiological approaches. The 36 articles address topics including nuclear genome organization; RNA stability and processing; splicing; translation; protein targeting in the chloroplast; photosystems; pigments; glycerolipids; the ATP synthase; and ferrodoxin and thioredoxin. Further contributions address new measurements methods for photosynthetic activity in vivo; starch biosynthesis; the responses of Chlamydomonas to various stress conditions; nitrogen assimilation; and mitochondrial genetics. Annotation copyrighted by Book News, Inc., Portland, OR

With the clear writing and accessible approach that have made it the authoritative introduction to the field of molecular photosynthesis, this fully revised and updated edition now offers students and researchers cutting-edge topical coverage of bioenergy applications and artificial photosynthesis; advances in biochemical and genetic methods; as well as new analytical techniques. Chapters cover the origins and evolution of photosynthesis; carbon metabolism; photosynthetic organisms and organelles; and the basic principles of photosynthetic energy storage. The book's website includes downloadable PowerPoint slides.

Plant productivity depends upon the photosynthetic conversion of the light energy into chemical energy stored in the biomass of plants. An intermediate step in this energy conversion process is electron transfer and proton translocation. At present, several research groups are working on projects that are expected to lead to rapid improvement of our understanding of the photosynthetic process. This book is a compilation of the work being done on the applications of molecular biology and bioenergetics of photosynthesis.

General Description of the Volume: The articles in this Methods in Enzymology volume reflect the integration of molecular genetics with photosynthesis. They focus on the genetic engineering of the photosynthesis apparatus, new mechanisms for genetic selection of photosynthetic mutants, biophysical and biochemical analysis of Photosystem I and Photosystem II Mutations, and Photosynthetic Gene Expression. General Description of the Series: The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences. Key Features * Genetic engineering of the photosynthesis apparatus * New mechanisms for genetic selection of photosynthetic mutants * Biophysical and biochemical analysis of Photosystem I and Photosystem II Mutations * Photosynthetic Gene Expression.

A comprehensive overview of various aspects of photosynthesis and its regulation is presented by this book. An international group of scientists offer historical background, recent data and new techniques and speculations on future research in such areas as: regulation of metabolism in chloroplasts and leaf cells; partitioning of carbon products; chloroplast genetics and molecular biology; photosynthetic electron transport; regulation of photosynthetic efficiency; C4-photosynthesis; ribulose bisphosphate carboxylase; photorespiration and photosynthesis; dark respiration; photosynthesis; and crop yield.

The Photosynthetic Apparatus: Molecular Biology and Operation: Cell Culture and Somatic Cell Genetics of Plants, Volume 7B is a collection of papers that discuss plastids – organelles found in plants that set them apart from other organisms. The book is divided into two parts. Coverage of Part I includes concepts such as photosynthesis and the photosynthetic apparatus - light energy and photosynthetic electronic transport, photosynthetic phosphorylation, and fractionation of the photosynthetic apparatus; photosystem II – its protein components, genetic aspects, and structure and function; the cytochrome b6/f complex; and the structure and function of coupling factor components. Coverage of Part II includes the biochemistry and molecular biology of chlorophyll; genes and enzymes for carotenoid biosynthesis; photoregulated development of chloroplasts; and the differentiation of amyloplasts and chromoplasts. The text is recommended for botanists, molecular biologists, and biochemists who are interested in the study of plant cells and photosynthesis.