Orchid Biochemistry 2 0

This reprint is a printed version of the Special Issue, entitled "Orchid Biochemistry 2.0", one literature review, and nine original research articles were published, and the Special Issue provides further insight into several critical subtopics, including reproduction biology, functional genomics in secondary metabolites, as well as polysaccharides and orchid mycorrhizae.

Orchids are fascinating, with attractive flowers that sell in the markets and an increasing demand around the world. Additionally, some orchids are edible or scented and have long been used in preparations of traditional medicine. This book presents recent advances in orchid biochemistry, including original research articles and reviews. It provides in-depth insights into the biology of flower pigments, floral scent formation, bioactive compounds, pollination, and plant–microbial interaction as well as the biotechnology of protocorm-like bodies in orchids. It reveals the secret of orchid biology using molecular tools, advanced biotechnology, multi-omics, and high-throughput technologies and offers a critical reference for the readers. This book explores the knowledge about species evolution using comparative transcriptomics, flower spot patterning, involving the anthocyanin biosynthetic pathways, the regulation of flavonoid biosynthesis, which contributes to leaf color formation, gene regulation in the biosynthesis of secondary metabolites and bioactive compounds, the mechanism of pollination, involving the biosynthesis of semiochemicals, gene expression patterns of volatile organic compounds, the symbiotic relationship between orchids and mycorrhizal fungi, techniques using induction, proliferation, and regeneration of protocorm-like bodies, and so on. In this book, important or model orchid species were studied, including Anoectochilus roxburghii, Bletilla striata, Cymbidium sinense, Dendrobium officinale, Ophrys insectifera, Phalaenopsis ‘Panda’, Pleione limprichtii.

This book provides comprehensive insights into the existing and emerging trends in orchid biology based on the findings of omics, high-throughput technology, biotechnology, molecular breeding, and genome editing approaches in orchids. It illustrates molecular mechanisms of orchid mycorrhizal symbiosis according to the recent achievements of transcriptomics and bioinformatics studies which accelerate the progress of orchid research with the aid of their high-throughput tools. In this book, a comprehensive view of orchid breeding was presented, and it includes fundamental methods as well as advanced strategies through the combination of several technologies such as genetic engineering, omics, computational biology, and genome editing. These resulting knowledge and tools are highly beneficial for obtaining novel and fascinating varieties in the orchid market which is a competitive industry of global trade. Another interesting content is the focus on the production of orchid bioactive compounds and their values in the field of ethnomedicine. Their sources chiefly came from secondary metabolites and can be enriched through elicitors and produced more efficiently by improved tissue culture protocols and bioreactors. In this edited collection, we provided space for presenting an updated review of in vitro seed germination which is a routine technology for well-trained researchers but can give a complete demonstration for the potential audiences including growers and research beginners. This book collects refined knowledge from a broad source of scientific literature by experts in the field of orchid research and surely is an adequate reference and textbook for students, teachers, and researchers. It includes methods and applications of orchid breeding technology which would gain high attention from growers, breeders, and the related fields of agriculture.

Orchid Biotechnology II presents a series of recent works on both basic and applied researches in biotechnology progress for Phalaenopsis and Oncidium orchids. These include the development of flower, ovule, gynostemium and perianth, the discovery of new orchid-infecting viruses and virus movement, secondary metabolites, technology of DNA endoduplication and genetic transformation, growth regulation by micronutrition and orchid mycorrhiza, and plant growth substances for flowering. The diversity and specialization in orchid floral morphology have fascinated botanists and collectors for centuries. The orchid industry has been growing substantially in the past ten years worldwide. This book focuses on the recent advances in the research of orchid biotechnology from the past ten years in Taiwan. To advance the orchid industry, enhancement of basic research as well as advanced biotechnology will provide a good platform to improve flower quality and breeding of new varieties.

This book provides information on genome complexity and evolution, transcriptome analysis, miRNome, simple sequence repeats, genome relationships, molecular cytogenetics, polyploidy induction and application, flower and embryo development. Orchids account for a great part of the worldwide floriculture trade both as cut flowers and as potted plants and are assessed to comprise around 10% of global fresh cut flower trade. A better understanding of the basic botanical characteristics, flower regulation, molecular cytogenetics, karyotypes and DNA content of important orchids will aid in the efficient development of new cultivars. The book also describes the composition, expression and function of various microRNAs and simple sequence repeats. Information on their involvement in all aspects of plant growth and development will aid functional genomics studies.