Orchid Biotechnology Ii

Orchid Biotechnology II presents a series of recent works on both basic and applied researches in biotechnology progress for Phalaenopsis and Oncidium orchids. These include the development of flower, ovule, gynostemium and perianth, the discovery of new orchid-infecting viruses and virus movement, secondary metabolites, technology of DNA endoduplication and genetic transformation, growth regulation by micronutrition and orchid mycorrhiza, and plant growth substances for flowering. The diversity and specialization in orchid floral morphology have fascinated botanists and collectors for centuries. The orchid industry has been growing substantially in the past ten years worldwide. This book focuses on the recent advances in the research of orchid biotechnology from the past ten years in Taiwan. To advance the orchid industry, enhancement of basic research as well as advanced biotechnology will provide a good platform to improve flower quality and breeding of new varieties.

This book provides a first hand and complete information on orchid biotechnology for orchid lovers, graduate students, researchers and industry growers. It contains comprehensive genomics and transcriptomics data, and a thorough discussion of the molecular mechanism of orchid floral morphogenesis. The contributors to the book are all orchid enthusiasts with more than 20 years' experience in the field. With more than 25,000 species, orchids are the most species-rich of all angiosperm families. They show wide diversity of epiphytic and terrestrial growth forms and have successfully colonized almost every habitat on earth. Orchids are fantastic for their spectacular flowers with highly evolved petal, labellum, and fused androecium and gynoecium, gynostemium, to attract pollinators for effective pollination. In addition, orchids have attracted the interest of many evolutionary biologists due to their highly specialized evolution and adaptation strategies. Orchid Biotechnology III covers the most update knowledge of orchid biotechnology research on Phalaenopsis, Oncidium, Cymbidium, Anoectohilus, Paphiopedilum, and Erycina pusilla. It will provide graduate students, researchers, orchid lovers and breeders with an opportunity to understand the mechanism why the orchids are so mysterious and spectacular. Hopefully, this information will be helpful for breeders to enhance orchid breeding and create even more elegant and grace flowers.

Orchid Biotechnology IV presents a series of recent work on both basic and applied researches in biotechnology progress for Phalaenopsis, Oncidium and Erycina pusila orchids. These include breeding of Phalaenopsis orchids of black flower, big-white flower and small and floriferous flowers, physiology for shipping and photosynthesis, SSR markers and mitochondrial DNA markers, virus detection and antiviral immunity, embyogenesis and relationship with mycorrhiza symbiosis, transposon and retrotransposon, orchid genome and evolution, regulation of orchid floral scent, floral color modification, and abiotic stress tolerance.The diversity and specialization in orchid floral morphology have fascinated botanists and collectors for centuries. The orchid industry has been growing substantially worldwide. To advance the orchid industry, enhancement of basic research as well as advanced biotechnology will provide a good platform to improve the flower quality and the breeding of new varieties. This book provides a first-hand and up-to-date information on orchid breeding, orchid genome evolution, detection of virus in nanotechnology, molecular markers for cultivar identification for orchid lovers, researchers and industry growers.

Orchid Biotechnology II presents a series of recent works on both basic and applied researches in biotechnology progress for Phalaenopsis and Oncidium orchids. These include the development of flower, ovule, gynostemium and perianth, the discovery of new orchid-infecting viruses and virus movement, secondary metabolites, technology of DNA endoduplication and genetic transformation, growth regulation by micronutrition and orchid mycorrhiza, and plant growth substances for flowering. The diversity and specialization in orchid floral morphology have fascinated botanists and collectors for centuries. The orchid industry has been growing substantially in the past ten years worldwide. This book focuses on the recent advances in the research of orchid biotechnology from the past ten years in Taiwan. To advance the orchid industry, enhancement of basic research as well as advanced biotechnology will provide a good platform to improve flower quality and breeding of new varieties.

This book on “Orchid Biology: Recent Trends & Challenges” reviews the latest strategies for the preservation and conservation of orchid diversity and orchid germplasm. It is an outcome of the Proceedings of the International Symposium on “Biodiversity of Medicinal Plants & Orchids: Emerging Trends and Challenges” held on 9-11 February 2018 at Acharya Nagarjuna University, India. In addition, eminent orchid experts from around the globe were invited to contribute to this book. All chapters were peer-reviewed by international experts. The Orchidaceae are one of the largest families of flowering plants, comprising over 700 genera and 22,500 species and contributing roughly 40 percent of monocotyledons. They also represent the second-largest flowering plant family in India, with 1,141 species in 166 genera, and contribute roughly 10% of Indian flora. Orchids comprise a unique group of plants and their flowers are among the most enchanting and exquisite creations of nature. Phylogenetically and taxonomically, the Orchidaceae are considered to be a highly evolved family among angiosperms. They show incredible diversity in terms of the shape, size and colour of their flowers, and are of great commercial importance in floriculture markets around the globe. Millions of cut flowers of Cymbidium, Dendrobium, Cattleya, Paphiopedilum, Phalaenopsis, Vanda etc., besides potted orchid plants, are sold in Western Countries and thus, the orchid cut flower industry has now become a multimillion-dollar business in Europe, the USA and South East Asia. Besides their ornamental value, orchids hold tremendous pharmaceutical potential. Root tubers of Habenaria edgeworthii form an important component of the ‘Astavarga’ group of drugs in Ayurvedic medicine. It is an established fact that tubers of some terrestrial orchids have been used to treat diarrhoea, dysentery, intestinal disorders, cough, cold and tuberculosis. Some orchids, particularly those belonging to the genera Aerides, Arachnis, Cattleya, Cymbidium, Dendrobium, Epidendrum, Oncidium, Paphiopedilum, Phalaenopsis, Renanthera, Vanda etc. have been extensively used to produce internationally acclaimed hybrids. Yet paradoxically, Indian orchids are victims of their own beauty and popularity. As a result, their natural populations have been declining rapidly because of unbridled commercial exploitation in India and abroad. In fact, some orchids are now at the verge of extinction, e.g. Renanthera imschootiana, Diplomeris hirsuta, Paphiopedilum fairrieanum, Cypripedium elegans, Taeniophylum andamanicum etc. Given the global importance of orchids in terms of securing human health and wealth, this comprehensive compilation, prepared by international experts, is highly topical. Its content is divided into five main sections: (I) Cryopreservation & Biotechnology, (II) Orchid Biodiversity & Conservation, (III) Anatomy & Physiology, (IV) Pollination Biology and (V) Orchid Chemicals & Bioactive Compounds. All contributions were written by eminent orchid experts/professors from around the world, making the book a valuable reference guide for all researchers, teachers, orchid enthusiasts, orchid growers and students of biotechnology, botany, pharmaceutical sciences and ethnomedicine. It will be equally valuable for readers from the horticultural industry, especially the orchid industry, agricultural scientists and policymakers.

This book provides comprehensive insights into the existing and emerging trends in orchid biology based on the findings of omics, high-throughput technology, biotechnology, molecular breeding, and genome editing approaches in orchids. It illustrates molecular mechanisms of orchid mycorrhizal symbiosis according to the recent achievements of transcriptomics and bioinformatics studies which accelerate the progress of orchid research with the aid of their high-throughput tools. In this book, a comprehensive view of orchid breeding was presented, and it includes fundamental methods as well as advanced strategies through the combination of several technologies such as genetic engineering, omics, computational biology, and genome editing. These resulting knowledge and tools are highly beneficial for obtaining novel and fascinating varieties in the orchid market which is a competitive industry of global trade. Another interesting content is the focus on the production of orchid bioactive compounds and their values in the field of ethnomedicine. Their sources chiefly came from secondary metabolites and can be enriched through elicitors and produced more efficiently by improved tissue culture protocols and bioreactors. In this edited collection, we provided space for presenting an updated review of in vitro seed germination which is a routine technology for well-trained researchers but can give a complete demonstration for the potential audiences including growers and research beginners. This book collects refined knowledge from a broad source of scientific literature by experts in the field of orchid research and surely is an adequate reference and textbook for students, teachers, and researchers. It includes methods and applications of orchid breeding technology which would gain high attention from growers, breeders, and the related fields of agriculture.

History, uniqueness, habit, habitat, orchid seed and their germination, distribution, evolutionary status, hybridization, registration of hybrids, taxonomy, methods of cultivation and orchid-mycorrhizal association are discussed to the extent necessary for a scientist. The status of world floriculture industry and India's position is also explained. The next focus of the book is on plant biotechnology. History, prospects, potentials and techniques are discussed in detail. Since the present trend in plant biotechnology is to produce genetically modified plants by transgenesis, importance has been given to the techniques related to this process. Methods of transgenesis, transgenic crops, and the related controversies are treated in separate chapters. Different methods by which foreign genes can be introduced into an organism are described with specific examples. These techniques are discussed for plant systems in general and also for orchids in particular. Protoplast isolation, fusion and culture techniques are also reviewed for plants in general and orchids with specific examples. A chapter dedicated to cytological techniques has also been introduced, which also includes the methods for the preparation of different types of stains used in cytology. Tissue culture technique forms the backbone of biotechnology and hence various steps of the technique are described in detail for orchids which of course is not much different for other plants. The last section carries protocols for the micropropagaion of four different orchid hybrids and also includes original results obtained during morphogenetic studies carried out by the author. The contents and design of the chapters in the book will meet the needs of students, researchers, trainees, professional scientists, and research scholars as well as to orchid lovers and floriculturists.