Practical Book Of Biotechnology Plant Tissue Culture

The book starts with an introduction to basic knowledge of instruments which deals with principle, working, uses, limitations and precautions of about ten instruments.Basic Knowledge of precaution of;Culture Media for Bacterial Growth, Plant Tissue Culture and Standard Solutions has been given in simple and easy-to-follow language.The biotechnology exercises such as Plasmid and DNA isolation, DNA size determination, Restriction digestion, PCR, Gus gene assay, RFLP, RAPD, Isolation of bacteria by streak and Pour plate method, Growth characteristics of E.Coli by Plating and Turbidimetric method and the plant tissues culture exercises such as Cell suspension culture, Androgenesis, Somatic embryogenesis, Preparation of plantlet to greenhouse field, have been given in a student friendly manner. Matter for Viva-voce has also been included.

This textbook is clearly structured with fourteen richly illustrated chapters and practical examples for easy understanding and direct implementation. The methods and findings developed in the authors’ group are presented in detailed, revised chapters. Readers will find valuable updates on the molecular basis of biotechnological processes, secondary metabolite production and genetic engineering. In addition, the basic principles of important biotechnologies, as well as examples of specially designed crops that deliver improved productivity under stress conditions, are presented. This second edition sets the direction for future research on the basic aspects of plant tissue culture and its applications in the fields of secondary metabolite production and genetic engineering. It provides both general and specific information for students, teachers, academic researchers and industrial teams who are interested in new developments in plant tissue culture and its applications.

Plant Biotechnology: Practical Manual covers most of the important areas of present-day plant biotechnology, beginning from plant tissue culture media preparation to transgenic plant production and related molecular biology protocols. It is meant for both students who are being introduced to plant biotechnology and those wanting to do advance research in this field. It would also be helpful for teachers in formulating their own practical protocols using different model plant systems. This book includes the principles, theoretical background and the basis for each protocol supported by the authors own research findings. This approach has been adopted to help the learners and researchers modify their procedures to develop their own protocols and methods utilizing the proven protocols included in the book.

Document from the year 2012 in the subject Agrarian Studies, , course: Carrier Oriented Program, language: English, abstract: Plant tissue culture is a collection of techniques used to maintain or grow plant cells, tissues or organs under sterile conditions on a nutrient culture medium of known composition. Different techniques in plant tissue culture may offer certain advantages over traditional methods of propagation. This practical manual has been prepared in response to the necessities of the graduate students as an introduction to the in vitro tissue culture techniques and some molecular aspects.

Plant Tissue Culture forms an integral basis of the present day biotechnology. Plant Tissue Culture: Practices and New Experimental Protocols is being brought out to fill the existing gap in the available literature on plant tissue culture, especially focusing on the aspects of practical procedures and protocols of tissue culture. This book contains important experimental techniques and gives guidance on carrying out hands-on experiences. It has been designed in a simple way, giving all the necessary procedures as a general guideline and also necessary tips to maneuver any problem encountered. These tips are based on the first hand experiences of the author while teaching and researching the techniques of plant tissue culture. A unique feature of this book is the inclusion of several techniques describing the actual protocols experimented and developed with different plant species by different scientists. A substantial number of original colored plates including fluorescence photographs standout the book. This pioneering work is valuable for the students who are looking for fresh outlook and search.

Plant cell culture techniques are used increasingly in basic research for plant exploitation in industry, including for example, genetic engineering and micropropagation. The rapidly developing role of plant cell culture has necessitated this new edition of a widely acclaimed book. It covers a wide range of methods central to the exploitation of plant cell cultures in fundamental and applied research. This thoroughly revised work retains the combination of giving and explaining the general principles involved with the concise description of specific protocols, with appeal to a broad readership, that made the first edition so successful. Internationally recognized experts describe the techniques used for isolating and manipulating cell cultures, and the central importance in plant biotechnology. The book will be of major interest to researchers in plant sciences in general, and specifically to botany, plant physiology, and biotechnology students.

Plant Tissue Culture, Second Edition is accompanied with new exercises demonstrating new arrays along with information on development of a customized protocol for protoplast isolation, suspension, haploid cultures, secondary metabolite production, and cryopreservation techniques. All experimental systems are written clear and easy-to-understand manner with the text being well-documented along with detailed drawings containing the plant tissue culture requirements for each particular application. Besides addressing recent advancements on wide variety of topics of Plant Tissue Culture, it gives the practical and technical knowledge required to train the next generation of plant scientists regardless of their ultimate specialization. It includes the complements of both theory and experiments. Plant Scientists, teachers and students will benefit greatly from this clearly presented tissue culture techniques that guides reader from lab setup to supplies, stock solution and media preparation, measurements, explant selection and disinfestations, along with their experimental observations.

Since the publication of the first edition in 1983, several new and exciting developments have taken place in the field of plant tissue culture, which forms a major component of what is now called plant biotechnology. The revised edition presents updated information on theoretical, practical and applied aspects of plant tissue culture. Each chapter has been thoroughly revised and, as before, is written in lucid language, includes relevant media protocols, and is profusely illustrated with self-explanatory diagrams and original photographs. This book includes three new chapters: "Variant selection", "Genetic Engineering" and "Production of Industrial Compounds" and contains a complete bibliography and a glossary of terms commonly used in tissue culture literature. This updated version proves to be an excellent text for undergraduate, postgraduate students and teachers in various fields of plant sciences and a useful reference book for those interested in the application of any aspect of this aseptic technology.