Recent Advances In Plant In Vitro Culture

The purpose of this book is to provide the advances in plant in vitro culture as related to perennial fruit crops and medicinal plants. Basic principles and new techniques, now available, are presented in detail. The book will be of use to researchers, teachers in biotechnology and for individuals interested to the commercial application of plant in vitro culture.

Advances in Plant Tissue Culture: Current Developments and Future Trends provides a complete and up-to-date text on all basic and applied aspects of plant tissue cultures and their latest application implications. It will be beneficial for students and early-career researchers of plant sciences and plant/agricultural biotechnology. Plant tissue culture has emerged as a sustainable way to meet the requirements of fresh produces, horticultural crops, medicinal or ornamental plants. Nowadays, plant tissue culture is an emerging filed applied in various aspects, including sustainable agriculture, plant breeding, horticulture and forestry. This book covers the latest technology, broadly applied for crop improvement, clonal propagation, Somatic hybridization Embryo rescue, Germplasm conservation, genetic conservation, or for the preservation of endangered species. However, these technologies also play a vital role in breaking seed dormancy over conventional methods of conservation. Focuses on plant tissue culture as an emerging field applied in various aspects, including sustainable agriculture, plant breeding, horticulture and forestry Includes current studies and innovations in biotechnology Covers commercialization and current perspectives in the field of plant tissue culture techniques

Recent Advances in Plant in Vitro Culture examines various aspects of vitro culture amongst the plants along with an introduction on plant cell culture. It includes the basic concepts of cell culture and its setting with the plant cell culture along with the description of synthetic seeds and organogenesis. Provide the reader with the insights into the development of plant cell culture, so as to understand the recent approaches undertaken in the field of Plant Tissue Cultures.

During the past decade, Plant Tissue Culture (PTC) has attracted considerable attention because of its vital role in plant biotechnology. PTC offers novel approaches to plant production, propagation, and preservation. Some in vitro techniques are being applied on a commercial scale while many others hold great potential. Consequently, the literature in this area has grown rapidly.This book deals with recent developments in plant tissue culture, and presents a critical assessment of the proven and potential applications of the various in vitro techniques, it also highlights current problems limiting the application of tissue culture, and projects the future lines of research in this field.

This book is divided into five sections. The first section deals with the methodology and bioresource generation, techniques related to genetic engineering, and gene transfer to the nuclear genome and chloroplast genome. The new techniques of genome profiling and gene silencing are also presented. The second section of the book covers the classical aspect of plant biotechnology viz. tissue culture and micropropagation. Use of genetic engineering via Agrobacterium and direct transfer of DNA through particle bombardment to develop transformed plants in Artemisia, castor and orchids, and production of recombinant proteins in plant cells have been dealt with in the third section. The fourth section addresses the abiotic and biotic stress tolerance in plants. The basic biology of some of the stress responses, and designing plants for stress tolerance is discussed in this section. The fifth section examines medicinal plants and alkaloid production.

The second edition of Experiments in Plant Tissue Culture makes available new information that has resulted from recent advances in the applications of plant tissue culture techniques to agriculture and industry. This comprehensive laboratory text takes the reader through a graded series of experimental protocols and also provides an introductory review of each topic. Topics include: a plant tissue culture laboratory, aseptic techniques, nutritional components of media, callus induction, organ formation, xylem cell differentiation, root cultures, cell suspensions, micropropagation, embryogenesis, isolation and fusion of protoplasts, haploid cultures, storage of plant genetic resources, secondary metabolite production, and quantification of procedures. This volume offers all of the basic experimental methods for the major research areas of plant tissue culture, and it will be invaluable to undergraduates and research investigators in the plant sciences.

Modern Applications of Plant Biotechnology in Pharmaceutical Sciences explores advanced techniques in plant biotechnology, their applications to pharmaceutical sciences, and how these methods can lead to more effective, safe, and affordable drugs. The book covers modern approaches in a practical, step-by-step manner, and includes illustrations, examples, and case studies to enhance understanding. Key topics include plant-made pharmaceuticals, classical and non-classical techniques for secondary metabolite production in plant cell culture and their relevance to pharmaceutical science, edible vaccines, novel delivery systems for plant-based products, international industry regulatory guidelines, and more. Readers will find the book to be a comprehensive and valuable resource for the study of modern plant biotechnology approaches and their pharmaceutical applications. Builds upon the basic concepts of cell and plant tissue culture and recombinant DNA technology to better illustrate the modern and potential applications of plant biotechnology to the pharmaceutical sciences Provides detailed yet practical coverage of complex techniques, such as micropropogation, gene transfer, and biosynthesis Examines critical issues of international importance and offers real-life examples and potential solutions

This manual provides all relevant protocols for basic and applied plant cell and molecular technologies, such as histology, electron microscopy, cytology, virus diagnosis, gene transfer and PCR. Also included are chapters on laboratory facilities, operation and management as well as a glossary and all the information needed to set up and carry out any of the procedures without having to use other resource books. It is especially designed for professionals and advanced students who wish to acquire practical skills and first-hand experience in plant biotechnology.