Recent Advances In Thin Layer Chromatography

For many years TLC has suffered from the image of being a low sensitivity, low resolution, non-quantitative teehnique, suitable for chemists, but not a tool for real ehromatographers. Whilst perhaps true in the past this attitude no longer ref1eets the eapabi1ities of modern instrumenta1ized TLC in all its many forms. This volume represents the proceedings of a meeting in Brighton in 1987 whieh formed part of a eontinuing series of one and two day events on TLC organized by the Chromatographie Soeiety either alone or, like this one, in eonjunetion with other learned bodies. These meetings are designed to keep ehromatographers up to date with the latest deve10pments and help promote a more positive image of TLC. lan Wilson November 1987 v CONTENTS The Chromatographic Society xi Contemporary Thin-layer Chromatography: An introduction IoD. Wilson INSTRUMENTATION Quantitative Methods in Thin-layer Chromatography C.F. Poole, S.K. Poole and T.A. Dean 11 Fluorescence Line-narrowing Spectroscopy: A New and Highly Selective Detection Technique for Thin-layer and Liquid Chromatography J.W. Hofstraat, C. Gooijer, U.A. Th. Brinkman and N.H. Velthorst 29 Qualitative and Quantitative Image Analysis of Fluorescence from High Performance Thi. n-layer Chromatography R.M. Belchamber, S.J. Brinkworth, H. Read and J.D.M. Roberts 37 Analytical Rotation Planar Chromatography Sz. Nyiredy, K. Dallenbach-Toelke and O. Sticher 45 Analytical and Preparative Overpressured Layer Chromatography E. Mincsovics and E. Tyihak 57 Anticircular Planar Chromatography: Analytical and Preparative Aspects H. Traitler and A.

Thin-layer chromatography (TLC) is widely used particularly for pharmaceutical and food analysis. While there are a number of books on the qualitative identification of chemical substances by TLC, the unique focus here is on quantitative analysis. The authors describe all steps of the analytical procedure, beginning with the basics and equipment for quantitative TLC followed by sample pretreatment and sample application, development and staining, scanning, and finally statistical and chemometric data evaluation and validation. An important feature is the coverage of effect-directed biological detection methods. Chapters are organized in a modular fashion facilitating the easy location of information about individual procedural steps.

Instrumental Thin-Layer Chromatography, Second Edition offers a comprehensive source of authoritative information on all aspects of instrumental thin-layer chromatography. The use of short, topic-focused chapters facilitates identifying information of immediate interest for familiar or emerging uses of thin-layer chromatography. The book gives those working in both academia and industry the opportunity to learn, refresh, or deepen their understanding of fundamental and instrumental aspects of thin-layer chromatography, as well as the tools to interpret and manage chromatographic data. The book serves as a practical consolidated guide to the selection of separation conditions and the use of auxiliary techniques. This fully updated new edition restores the contemporary character of the book for those involved in advancing the technology, analyzing data produced, or applying the technique to new application areas. Some chapters have been consolidated to make room for topics not covered in the first edition, reflecting general changes in the field of thin-layer chromatography, especially in effects-directed detection, convenient interfaces for advanced spectroscopic detection, and greater automation possibilities. This book is a valuable reference for anyone who needs to acquire fundamental and practical information to facilitate progress in research and management functions utilizing information acquired by thin-layer chromatography. Features individual chapters written by recognized authoritative and visionary experts in the field Provides an overview and focused treatment of a single topic Provides tables and diagrams with commonly used data to facilitate practical work, comparison of results, and decision-making Places modern developments in the research literature into a general context not always apparent to inexperienced users of the technique Offers comprehensive updates to all chapters Includes new chapters on instrument platforms, effects-directed detection, data analysis tools, small-scale and microfluidic planar separation systems, and applications to the separation of amino acids and peptides, the analysis of saccharides and lipids, and forensic analysis

Thin-layer chromatography (TLC) is a powerful, fast and inexpensive analytical method. It has proven its usefulness in pharmaceutical, food and environmental analysis. This new edition of the practical TLC guide features a completely revised chapter on documentation, now including the use of digital cameras. Selected new sorbents and instruments are also introduced. Why has the prior edition been successful? All steps of the analytical procedure are clearly explained, starting with the choice of a suitable TLC technique and ending with data evaluation and documentation. Special emphasis is put on the proper choice of materials for TLC. Properties and functions of various materials and the TLC equipment are described, covering e. g. precoated layers, solvents and developing chambers, including information on suppliers. Many practical hints for trouble shooting are given. All this is illustrated with numerous coloured figures. How to use TLC in compliance with GLP/GMP regulations is described in detail, including the required documentation. Therefore the reader can very easily compile his own standard operating procedures.

Thin-Layer Chromatography (TLC) is a modern, reliable tool that complements other chromatographic techniques. This book provides a practical guide to the basic principles, procedures and pitfalls on the practical application of TLC. Thin Layer Chromatography: A Modern Practical Approach offers a sequence of chapters following the steps of the technique as the chromatographer would follow them. The chapters provide a choice of sorbent best suited to the separation intended, followed by pre-treatment required for the sample, applying the sample to the sorbent layer, development procedure, visualisation and detection, and finally quantification. Imaging and hyphenation techniques are described. The reasons why recommendations are made for specific and more general methods are covered. The book also provides an overview of some recent developments in the field.

A practical how-to guide to all the basic techniques needed to practice thin layer chromatography in biochemical/pharmaceutical research and quality control. This updated edition presents the most current techniques as well as the hows and whys of TLC. Provides step-by-step methods for performing the separations as well as doing related tasks, such as applying the sample, selecting the mobile phase, and quantitation. Includes a special chapter on how to select solvents for the development of a chromatogram to separate specific individual components of a mixture.