RNAi Technology

RNAi Technology
Author: R. K. Gaur,Yedidya Gafni,P. Sharma,V. K. Gupta
Publsiher: CRC Press
Total Pages: 380
Release: 2016-04-19
Genre: Science
ISBN: 9781439873250

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RNAi technology is used for large-scale screens that systematically shut down each gene in the cell, which can help identify the components necessary for a particular cellular process or an event such as cell division. Exploitation of the pathway is also a promising tool in biotechnology and medicine. Introducing new technology in the study of RNA

Gene Silencing by RNA Interference

Gene Silencing by RNA Interference
Author: Muhammad Sohail
Publsiher: CRC Press
Total Pages: 398
Release: 2004-08-27
Genre: Medical
ISBN: 9780203489253

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Maximizing the potential of RNA interference in functional genomics - as well as in the development of therapeutics - continues to be at the forefront of biomedical research. Unlike journal articles, Gene Silencing by RNA Interference: Technology and Application combines essential background to the RNAi field with practical techniques designed by r

RNA Interference

RNA Interference
Author: Dr. Tim Doran,Chris Helliwell
Publsiher: CABI
Total Pages: 269
Release: 2009
Genre: Science
ISBN: 9781845934101

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This methods manual provides an introduction to RNA interference, the theory behind its many applications, and specific protocols for RNAi, in organisms from plants and C.elegans to Drosophila and mammals. There are also chapters covering small hairpin RNAs and viral-induced gene silencing.

RNA Interference in Practice

RNA Interference in Practice
Author: Ute Schepers
Publsiher: John Wiley & Sons
Total Pages: 336
Release: 2006-03-06
Genre: Science
ISBN: 9783527604371

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This hands-on guide to RNA interference brings the power of targeted gene silencing to any laboratory with the basic equipment for handling nucleic acids. In easy-to-follow, step-by-step protocols you will learn * how RNAi works in worms, flies and mammals, * how to design the most efficient RNAi constructs, * how to achieve transient, stable and conditional RNAi in cell cultures, * how to determine the efficiency of an RNAi experiment, * and how to use RNAi for gene therapy. All the protocols have been thoroughly tested in the author's own laboratory, and she provides examples of successful experiments and troubleshooting hints to help in establishing your own successful RNAi experiments. Also includes a list of suppliers for RNAi reagents and equipment as well as a glossary of terms.

RNA Interference

RNA Interference
Author: Patrick J. Paddison,Peter K. Vogt
Publsiher: Springer Science & Business Media
Total Pages: 277
Release: 2008-01-01
Genre: Medical
ISBN: 9783540751571

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In the last few years the major effect that RNAi has had in invertebrate systems is beginning to take hold in mammalian systems through both single gene knockdown experiments and genome-scale screens. In the next decade, there will no doubt be both notable successes and failures as we attempt to apply this genetic tool to various biological problems. Through the introduction of RNAi, mammalian systems have finally gained admittance to the pantheon of model genetic systems.

Cellular RNA Interference Mechanisms

Cellular RNA Interference Mechanisms
Author: Anonim
Publsiher: Academic Press
Total Pages: 261
Release: 2011-08-12
Genre: Science
ISBN: 9780124158610

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This volume explores some of the most exciting recent advances in basic research on cellular RNA interference mechanisms and how this knowledge is leading to advances in the various fields. This series provides a forum for discussion of new discoveries, approaches, and ideas Contributions from leading scholars and industry experts Reference guide for researchers involved in molecular biology and related fields

RNAi

RNAi
Author: Gregory J. Hannon
Publsiher: CSHL Press
Total Pages: 568
Release: 2003
Genre: Gene silencing
ISBN: 0879696419

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In the past three years, the use of double-stranded RNA to silence gene activity has become widely and rapidly adopted. RNA interference is highly specific and remarkably potent, and it acts on cells and tissues far removed from the site of introduction. The principles behind RNAi are just being uncovered, but this laboratory technique has been applied effectively in a wide variety of animal and plant species. Variations on RNAi are revolutionizing many approaches to experimental biology, complementing traditional genetic technologies with a quicker and less expensive way of mimicking the effects of mutations both in cell cultures and in living animals. Recent advances in the use of RNAi to engineer heritable silencing in mammals, to alter stem cells for organ reconstitution, and to alter the course of disease in model systems indicate that RNAi may have a future in disease therapy. Written by pioneers in this new field and edited by Gregory Hannon, one of its leading figures, RNAi: A Guide to Gene Silencing presents the principles of RNAi and reliable protocols for its laboratory use in Caenorhabditis elegans, Drosophila, plants, avian embryos, mammalian cells, mouse oocytes, and more. This important and unique book is an essential laboratory resource for scientists studying gene regulation and for all experimental biologists interested in the emerging practical applications of RNAi.

RNA Interference

RNA Interference
Author: Ibrokhim Y. Abdurakhmonov
Publsiher: BoD – Books on Demand
Total Pages: 460
Release: 2016-04-06
Genre: Medical
ISBN: 9789535122722

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RNA interference (RNAi), a hallmark of all biological sciences of twenty-first century, is an evolutionarily conserved and double-stranded RNA-dependent eukaryotic cell defense process. Opportunity to utilize an organisms own gene and to systematically induce and trigger RNAi for any desired sequence made RNAi an efficient approach for functional genomics, providing a solution for conventional longstanding obstacles in life sciences. RNAi research and application have significantly advanced during past two decades. This book RNA interference provides an updated knowledge and progress on RNAi in various organisms, explaining basic principles, types, and property of inducers, structural modifications, delivery systems/methodologies, and various successful bench-to-field or clinic applications and disease therapies with some aspects of limitations, alternative tools, safety, and risk assessment.