Robotic Micromanipulation Of Zebrafish Larva

This book offers readers a series of robotic methods for manipulating zebrafish larva, one of the most popular model vertebrates widely used in biomedical research and clinical applications. The authors leverage advanced control theories, image processing algorithms, and artificial intelligence to establish a robot-assisted automated or semi-automated zebrafish larva-targeted micromanipulation system for different experimental purposes. The methods presented are generic and can be translated to manipulate other types of biological objects, such as embryos or cells. Coverage includes topics that span the procedures of manipulating zebrafish larva, such as in-plane positioning, three-dimensional orientation, deformation-controllable immobilization, organ-targeted microinjection, whole-organism imaging, and high-throughput trajectory tracking of zebrafish larvae group movement. Robotic Micromanipulation of Zebrafish Larva is written in a simple, clear, and easy-to-read style. It is an ideal reference for academic researchers and biomedical operators. It is also a valuable resource for students learning robotics, control and system theories, image processing, artificial intelligence, and biomedical engineering.

Robotics for Cell Manipulation and Characterization provides fundamental principles underpinning robotic cell manipulation and characterization, state-of-the-art technical advances in micro/nano robotics, new discoveries of cell biology enabled by robotic systems, and their applications in clinical diagnosis and treatment. This book covers several areas, including robotics, control, computer vision, biomedical engineering and life sciences using understandable figures and tables to enhance readers’ comprehension and pinpoint challenges and opportunities for biological and biomedical research. Focuses on, and comprehensively covers, robotics for cell manipulation and characterization Highlights recent advances in cell biology and disease treatment enabled by robotic cell manipulation and characterization Provides insightful outlooks on future challenges and opportunities

Micro and Nano Systems for Biophysical Studies of Cells and Small Organisms provides a comprehensive introduction to the state-of-the-art micro and nano systems that have recently been developed and applied to biophysical studies of cells and small organisms. These micro and nano systems span from microelectromechanical systems (MEMS) and microfluidic devices to robotic micro-nanomanipulation systems. These biophysical studies range from cell mechanics to the neural science of worms and Drosophila. This book will help readers understand the fundamentals surrounding the development of these tools and teach them the most recent advances in cellular and organismal biophysics enabled by these technologies. Comprehensive coverage of micro and nano-system technology and application to biophysical studies of cells and small organisms. Highlights the most recent advances in cellular and organismal biophysics enabled by micro and nano systems. Insightful outlook on future directions and trends in each chapter covering a sub-area of the book topic.

This book provides an overview of the noteworthy developments in the field of micromachining, with a specific focus on microinjection systems used for biological micromanipulation. The author also explores the design, development, and fabrication of new mechanical designs for micromachines, with plenty of examples that elucidate their modeling and control. The design and fabrication of a piezoelectric microinjector, constant force microinjector, constant force microgripper, PDVF microforce sensor, and a piezoelectric microsyringe are presented as examples of new technology for microinjection systems. This book is appropriate for both researchers and advanced students in bioengineering.

Francis BACON, in his Novum Organum, Robert BOYLE, in his Skeptical Chemist and René DESCARTES, in his Discourse on Method; all of these men were witnesses to the th scientific revolution, which, in the 17 century, began to awaken the western world from a long sleep. In each of these works, the author emphasizes the role of the experimental method in exploring the laws of Nature, that is to say, the way in which an experiment is designed, implemented according to tried and tested te- niques, and used as a basis for drawing conclusions that are based only on results, with their margins of error, taking into account contemporary traditions and prejudices. Two centuries later, Claude BERNARD, in his Introduction to the Study of Experimental Medicine, made a passionate plea for the application of the experimental method when studying the functions of living beings. Twenty-first century Biology, which has been fertilized by highly sophisticated techniques inherited from Physics and Chemistry, blessed with a constantly increasing expertise in the manipulation of the genome, initiated into the mysteries of information techn- ogy, and enriched with the ever-growing fund of basic knowledge, at times appears to have forgotten its roots.

This fully updated edition introduces new tools, models, and analytic insights that position the zebrafish even more strongly as an engine of discovery for developmental and disease biology. Beginning with a section exploring detailed methods for use of zebrafish to model a variety of human diseases, the book continues by illuminating the key ongoing role of the fish model in studies of the vertebrate nervous system, tools and approaches using zebrafish to study stem cell and regenerative biology, as well as techniques in genetics and genomics. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Zebrafish: Methods and Protocols, Third Edition serves as an invaluable guide to propel advances in developmental biology, disease modeling, and regeneration research using zebrafish and medaka as model systems.

Mikro- und Nanotechnik haben Wissenschaft und Forschung revolutioniert. In Zukunft werden sie auch den Alltag verändern. Nun liegt der erste Band einer neuen Buchreihe vor: Advanced Micro and Nano Systems 1. Henry Baltes und seine Co-Autoren knüpfen mit AMN an die Sensors Update-Reihe an. Das Autorenteam wurde um weitere Experten erweitert. AMN wird zwei Mal pro Jahr mit einem neuen Band die aktuellen Entwicklungen in der Mikro- und Nano-Welt begleiten. Die Erforschung und der Einsatz von Mikro- und Nanosystemen sind eines der brandaktuellen Themen im Wissenschaftsbereich. Die Forschungsergebnisse werden mehr und mehr auch konkret umgesetzt. Damit werden Mikro- und Nanotechnologie zu Wirtschaftsfaktoren. Aktuelle Entwicklungen, neue Technologien, Nano-Bauelemente und Systeme im Mikromaßstab - Advanced Micro and Nano Systems, die neue Buchreihe, wird Spiegel der spannenden und faszinierenden Mikro- und Nano-Welt sein. Zweimal pro Jahr wird es einen neuen AMN-Band geben. Die Autoren sind ausgewiesene Spezialisten. Zu den Herausgebern zählt Henry Baltes, Professor an der ETH Zürich. Er zeichnete bereits für die Bände der Sensors Update-Reihe verantwortlich. Die Artikel ermöglichen Neueinsteigern einen ersten Zugriff auf die Materie. Fachleute erhalten einen umfassenden Überblick. Anspruch der Herausgeber ist es, nicht nur die theoretischen Grundlagen von Mikro- und Nanosystemen zu reflektieren, sondern immer auch praktische Möglichkeiten und die Grenzen der Anwendung im Blick zu haben. Die AMN-Bände sind Handbücher und Nachschlagewerke in einem. Die Reihe richtet sich an Vertreter unterschiedlicher Fachrichtungen: Biologie, Chemie, Mathematik, Sensorindustrie und Materialwissenschaften.

A second edition of the classic handbook has become a standard in the Drosophila field. This edition is expanded to include topics in which classical genetic strategies have been augmented with new molecular tools. Included are such new techniques as homologous recombination, RNAi, new mapping techniques, and new mosaic marking techniques.