Separation Of Molecules Macromolecules And Particles

Providing chemical engineering undergraduate and graduate students with a basic understanding of how separation of a mixture of molecules, macromolecules or particles is achieved, this textbook is a comprehensive introduction to the engineering science of separation. • Students learn how to apply their knowledge to determine the separation achieved in a given device or process • Real-world examples are taken from biotechnology, chemical, food, petrochemical, pharmaceutical and pollution control industries • Worked examples, elementary separator designs and chapter-end problems are provided, giving students a practical understanding of separation. The textbook systematically develops different separation processes by considering the forces causing the separation and how this separation is influenced by the patterns of bulk flow in the separation device. Readers will be able to take this knowledge and apply it to their own future studies and research in separation and purification. Online resources include solutions to the exercises and guidance for computer simulations.

Describes partition techniques for the separation and purification of cells, cell organelles, membrane vesicles, viruses, and biopolymers, such as proteins and nucleic acids. The basic theory of partition is discussed, as are the properties of aqueous, two-phase systems and the general behavior of particles and molecules. Updates include advances and new applications introduced since 1971.

Particle Separation Techniques: Fundamentals, Instrumentation, and Selected Applications presents the latest research in the field of particle separation methods. This edited book authored by subject specialists is logically organized in sections, grouping the separation techniques according to their preparative or analytical purposes and the particle type. Along with the traditional and classical separation methods suitable for micronic particles, an update survey of techniques appropriate for nanoparticle characterization is presented. This book fills the gap in the literature of particle suspension analysis of a synthetic but comprehensive manual, helping the reader to identify and apply selected techniques.It provides an overview of the techniques available to a reader who is not an expert on particle separation yet about to enter the field, design an experiment, or buy an instrument for his/her new lab. Presents a resource that is ideal for anyone preparing samples across a variety of fields, including pharmaceuticals, food science, pollution analysis and control, agricultural products, and more Includes real case examples discussed by leading experts in the field Provides chapters that contain a unique, common table that summarizes points-of-strength and the weaknesses of each technique

Centrifugal Separations in Molecular and Cell Biology focuses on the application of modern centrifugation technology in molecular and cell biology, including the separation and fractionation of biological particles by centrifugation on the preparative and analytical scales. The selection first covers the principles and practices of centrifugation and the bases of centrifugal separations. Discussions focus on the basic concepts of sedimentation theory, centrifugation methods, designing centrifugation experiments, care of centrifuges and rotors, and statistical estimation of molecular parameters. The book also ponders on the practical aspects of rate-zonal centrifugation, including gradient materials, density and viscosity of glycerol solutions, and resolution and gradient shape. The publication examines fractionations in zonal rotors and the quantitative aspects of rate-zonal centrifugation. The text then reviews isopycnic centrifugation in ionic media and analytical centrifugation. Topics include separation by isopycnic banding, large-scale preparative procedures, and density-gradient solutes. The selection is a valuable reference for readers interested in centrifugation technology.

The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. More than 260 volumes have been published (all of them still in print) and much of the material is relevant even today--truly an essential publication for researchers in all fields of life sciences. Key Features * Liquid chromatography * Electrophoresis * Mass spectrometry.

Completely revised to reflect the innovations in HPLC from the past decade, this authoritative reference presents practical strategies for the evaluation and analysis of proteins, peptides, and polynucleotides. Offering class-specific applications for the characterization and fractionation of biological macromolecules, the book contains material on organic supports, size exclusion, ion exchange, hydrophobic interaction, and metal interaction chromatography. Leading experts summarize specialized detection systems, provides discussions on the chemical and biological properties of specific biomolecules, include detailed guidelines for the development of analytical techniques, and more.

A unique text providing comprehensive coverage of fundamental particle science, processing and technology. Including quantitative tools, real-world case studies and end-of-chapter problems, it is ideal for students in engineering and applied sciences, as well as for practitioners in a range of industries manufacturing particulate products.