The Enzyme Linked Immunosorbent Assay Elisa
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Enzyme linked Immunosorbent Assay ELISA
Author | : Samira Hosseini,Patricia Vázquez-Villegas,Marco Rito-Palomares,Sergio O. Martinez-Chapa |
Publsiher | : Springer |
Total Pages | : 115 |
Release | : 2017-12-30 |
Genre | : Technology & Engineering |
ISBN | : 9789811067662 |
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This book offers comprehensive information on all aspects of ELISA, starting with the fundamentals of the immune system. It also reviews the history of analytical assays prior to the advent of ELISA (enzyme-linked immunosorbent assay) and addresses the materials of choice for the fabrication of the platforms, possible biomolecular interactions, different protocols, and evaluation parameters. The book guides readers through the respective steps of the analytical assay, while also familiarizing them with the possible sources of error in the assay. It offers detailed insights into the immobilization techniques used for protein attachment, as well as methods for evaluating the assay and calculating the key parameters, such as sensitivity, specificity, accuracy and limit of detection. In addition, the book explores the advantages and shortcomings of the conventional ELISA, as well as various approaches to improving its performance. In this regard, merging and integrating other technologies with widely known ELISAs have opened new avenues for the advancement of this immunoassay. Accordingly, the book provides cutting-edge information on integrated platforms such as ELISpot, plasmonic ELISAs, sphere-/bead-based ELISAs, paper-/fiber-based ELISAs and ELISA in micro-devices.
ELISA
Author | : John R. Crowther |
Publsiher | : Springer Science & Business Media |
Total Pages | : 231 |
Release | : 2008-02-02 |
Genre | : Medical |
ISBN | : 9781592595297 |
Download ELISA Book in PDF, Epub and Kindle
ELISA: Theory and Practice introduces to scientists at all levels of expertise the principles of the most commonly used assay technique known as the Enzyme Linked Immunosorbent Assay. The book provides readers with full descriptions of the basic systems that make ELISA one of the most powerful techniques in science today, and also examines in detail the data obtained by ELISA and their analysis and actual manipulation. ELISA: Theory and Practice is designed not only to train novices in the science of ELISA, but also to aid investigators experienced in any of the biological sciences in performing independently assays of antibodies and antigens. Mastery of the book's contents will allow readers to fully appreciate exactly how and why assays function, as well as permit the efficient development of individual assays that are both rapid and accurate.
The enzyme linked immunosorbent assay ELISA a review of recent developments with abstracts of microplate applications 2 1980
Author | : Alister Voller,Dennis Bidwell |
Publsiher | : Unknown |
Total Pages | : 128 |
Release | : 1980 |
Genre | : Immunoenzyme technique |
ISBN | : OCLC:622328978 |
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Enzyme Immunoassay
Author | : Eiji Ishikawa,Tadashi Kawai,Kiyoshi Miyai |
Publsiher | : Igaku-Shoin Medical Publishers |
Total Pages | : 298 |
Release | : 1981 |
Genre | : Science |
ISBN | : UOM:39015001929523 |
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Techniques in Molecular Biology
Author | : J.M. Walker,W. Gaastra |
Publsiher | : Springer Science & Business Media |
Total Pages | : 304 |
Release | : 2012-12-06 |
Genre | : Science |
ISBN | : 9789401165631 |
Download Techniques in Molecular Biology Book in PDF, Epub and Kindle
The last few years have seen the rapid development of new methodology in the field of molecular biology. New techniques have been regularly introduced and the sensitivity of older techniques greatly improved upon. Developments in the field of genetic engineering in particular have contributed a wide range of new techniques. The purpose of this book therefore is to introduce the reader to a selection of the more advanced analytical and preparative techniques which the editors consider to be frequently used by research workers in the field of molecular biology. In choosing techniques for this book we have obviously had to be selective, and for the sake of brevity a knowledge of certain basic biochemical techniques and terminology has been assumed. However, since many areas of molecular biology are developing at a formidable rate and constantly generating new terminology, a glossary of terms has been included. The techniques chosen for this book are essentially based on those used in a series of workshops on 'techniques in molecular biology' that have been held at The Hatfield Polytechnic in recent years. In choosing these chapters we have taken into account many useful suggestions and observations made by participants at these workshops. Each chapter aims to describe both the theory and relevant practical details for a given technique, and to identify both the potential and limitations of the technique. Each chapter is written by authors who regularly use the technique in their own laboratories.
Basic Science Methods for Clinical Researchers
Author | : Morteza Jalali,Francesca Yvonne Louise Saldanha,Mehdi Jalali |
Publsiher | : Academic Press |
Total Pages | : 382 |
Release | : 2017-03-31 |
Genre | : Science |
ISBN | : 9780128030783 |
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Basic Science Methods for Clinical Researchers addresses the specific challenges faced by clinicians without a conventional science background. The aim of the book is to introduce the reader to core experimental methods commonly used to answer questions in basic science research and to outline their relative strengths and limitations in generating conclusive data. This book will be a vital companion for clinicians undertaking laboratory-based science. It will support clinicians in the pursuit of their academic interests and in making an original contribution to their chosen field. In doing so, it will facilitate the development of tomorrow’s clinician scientists and future leaders in discovery science. Serves as a helpful guide for clinical researchers who lack a conventional science background Organized around research themes pertaining to key biological molecules, from genes, to proteins, cells, and model organisms Features protocols, techniques for troubleshooting common problems, and an explanation of the advantages and limitations of a technique in generating conclusive data Appendices provide resources for practical research methodology, including legal frameworks for using stem cells and animals in the laboratory, ethical considerations, and good laboratory practice (GLP)
The Enzyme Linked Immunosorbent Assay ELISA
Author | : A. Voller,D. E. Bidwell,A. Bartlett |
Publsiher | : Unknown |
Total Pages | : 130 |
Release | : 1979 |
Genre | : Medical |
ISBN | : CORNELL:31924050562838 |
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Enzyme Linked Immunosorbent Assay
Author | : Sarika Mohan |
Publsiher | : Elsevier Health Sciences |
Total Pages | : 12 |
Release | : 2019-10-31 |
Genre | : Medical |
ISBN | : 9788131260258 |
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Enzyme Linked Immunosorbent Assay