The Mycobacteria

Updated and revised, this thorough volume provides a selection of the newest methods, as well as some of the basic methods required for a mycobacterial research laboratory. Mycobacteria Protocols, Third Edition guides readers through fractionation and analysis of macromolecules, from nucleic acids to proteins, complex lipids, and metabolites. Detailed and comprehensive protocols are provided for protein and lipid/glycolipid analysis using well-established methods; these are now complemented by a metabolomics chapter in which the complement of metabolites can be profiled. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Mycobacteria Protocols, Third Edition will be a resource both to those working in the field and to newcomers.

Tuberculosis continues to kills more people than any other single infective agent. The resurgence of the disease in many countries has produced a heightened awareness of the threat posed by mycobacterial infections. At the same time, there has been an explosion of knowledge of the fundamental properties of mycobacteria, most notably the determination of the complete genome sequence of Mycobacterium tuberculosis. This book provides an up-to-date account of these developments in the molecular biology and immunology of mycobacteria, coupled with allied advances of a more applied nature, such as the use of molecular techniques for diagnosis and epidemiological investigations. With chapters contributed from an international team of experts, it will not only be an essential reference text for the expanding mycobacterial research community, but also will find a prominent place on the shelves of clinicians, infectious disease and public health specialists, diagnostic laboratories, postgraduate students and indeed anyone concerned with the management and investigation of outbreaks of tuberculosis. Comprehensively covers recent advances in the molecular biology of the mycobacteria. First book to be published on this subject since the publishing of the complete genome for the tubercle bacillus (M. tuberculosis). Coincides with a worldwide resurgence of tuberculosis.

This book is a comprehensive and authoritative source on nontuberculous mycobacterial (NTM) pathogens and diseases and their appropriate management, with a focus on lung disease. NTM diseases, especially lung diseases, are increasing in prevalence in the U.S. and internationally with concomitant growing interest in a broad section of the medical community. Often merely included in coverage of tuberculosis, many aspects of NTM organisms and diseases are actually very different than TB. These differences are not intuitive or trivial and frequently result in suboptimal management of NTM patients. This book addresses these gaps in the literature with chapters on microbiology, pathophysiology, epidemiology, the various diseases that can stem from NTM, and their particular management. There is also coverage on prevention and NTM as a public health problem. For pulmonologists and infectious disease physicians, this is the definitive resource on nontuberculous mycobacteria.

This work contains updated and clinically relevant information about tuberculosis. It is aimed at providing a succinct overview of history and disease epidemiology, clinical presentation and the most recent scientific developments in the field of tuberculosis research, with an emphasis on diagnosis and treatment. It may serve as a practical resource for students, clinicians and researchers who work in the field of infectious diseases.

Completely revised and updated, this comprehensive resource covers the epidemiology, pathogenesis, varied clinical manifestations, treatment and prevention of tuberculosis. All of the extra-pulmonary as well as pulmonary manifestations of tuberculosis are covered. Atypical mycobacterial infection is detailed. A special feature of this second edition is the new material on AIDS, which is now known to be complicated by tuberculosis infection.

habitats and the overlapping of these biotopes by humans and animals contributed to the spread of mycobacteria and perhaps to their convergence to pathogenicity. It was not our intention to present a compendium covering all published results, but rather to issue a “readable” book, which is illustrative and thus focused on the principle facts. The increase in the number of Editors has allowed the sharing of original experiences regarding the ecology of mycobacteria, published here for the ?rst time in some cases. The supplemented edition should serve as a guide to these discoveries and also contribute to an understanding of clinically signi?cant species in human and animal medicine. Borstel, Germany, January 2009 Jindrich Kazda Editors’Comments The editors responsible for the chapters are listed under the title of each chapter. Authors are listed under the titles of subchapters. The references are listed as they appear in the databases Reference Manager (Thomson Reuters, Philadelphia) as imported from Web of Science (Thomson Reuters, Philadelphia) or PubMed (Medline, NLM Bethesda). A few citations, not indexed, were cited according to the reprints or books available. This principle resulted in minor differences in the titles (not all reference titles are in English, some references have capitalized title words, not all species names are according to the contemporary nomenclature and in italics). Some journals are cited with abbreviated titles, some in full, as available in the source databases. These differences were left in the format of the database.