Theory Practice Of Histotechnology

This is a brand new edition of the leading reference work on histological techniques. It is an essential and invaluable resource suited to all those involved with histological preparations and applications, from the student to the highly experienced laboratory professional. This is a one stop reference book that the trainee histotechnologist can purchase at the beginning of his career and which will remain valuable to him as he increasingly gains experience in daily practice. Thoroughly revised and up-dated edition of the standard reference work in histotechnology that successfully integrates both theory and practice.Provides a single comprehensive resource on the tried and tested investigative techniques as well as coverage of the latest technical developments. Over 30 international expert contributors all of whom are involved in teaching, research and practice.Provides authoritative guidance on principles and practice of fixation and staining. Extensive use of summary tables, charts and boxes.Information is well set out and easy to retrieve. Six useful appendices included (SI units, solution preparation, specimen mounting, solubility). Provides practical information on measurements, preparation solutions that are used in daily laboratory practice. Color photomicrographs used extensively throughout. Better replicates the actual appearance of the specimen under the microscope. Brand new co-editors. New material on immunohistochemical and molecular diagnostic techniques.Enables user to keep abreast of latest advances in the field.

This leading reference work on histological techniques is an essential and invaluable resource no matter what part you play in histological preparations and applications, whether you're a student or a highly experienced laboratory professional.

This fifth edition of Histological and Histochemical Methods continues to provide a clear and consistent introduction to the techniques, description and analysis of the chemical and physical principles of fixation, tissue processing, staining, enzyme location, immunohistochemistry and other key procedures. The overall structure of the book remains unchanged, but the content has been heavily revised to update the techniques used in line with recent technological advances. Additionally, there are new sections on: Artefacts and troubleshooting Methods for microorganisms and fungi in sections Methods for various pigments and mineral deposits in tissues Methods for skeletal elements (bone, cartilage) in whole-mounts Histological and Histochemical Methods 5e is essential reading for students, lecturers, researchers and professionals using histological and histochemical techniques. From reviews: "Histological and Histochemical Methods is a tour de force wholly suited to the modern age of histology and Professor Kiernan has triumphed again. To cover so much ground clearly and concisely while including the justification of the underlying chemistry makes this book unique. There should not be a histology laboratory or an undergraduate library that does not own a copy." Biotechnic & Histochemistry 2016, 91(2): 145. "This book should be present on the bookshelves of every research or analysis laboratory where histology and histochemistry are routinely used, as an essential reference source of basic and practical information for scientists and technicians." European Journal of Histochemistry, 2016, vol. 60.

Revised and updated edition (1st was 1981) of a textbook on chemical and physical principles of fixation, staining and histochemistry. For students i all biological subjects using histological techniques, as well as researcher and medical laboratory technologists. Annotation copyright Book News, Inc

本书内容全面,涵盖了组织学技术的方方面面,既包括传统的常用技术,又介绍了近年来新发展的,有较高实用价值的新技术。各章节分别论述了光镜、组织固定、样本处理、冰冻切片、石蜡切片、酶组织学、免疫组化、免疫荧光、组织芯片、原位杂交、荧光原位杂交、激光显微解剖、电镜等技术;对于结缔组织、糖类、脂类、蛋白质、核酸、色素及矿物质、淀粉、神经内分泌颗粒、微体、骨等相关的组绢学技术也予专门章节讲述;另外还涉及了显微镜样品定量、实验室安全及管理,以及人体工程学等相关内容。

This book has been completely updated in the new 3rd edition. New coverage includes chapters on immunohistochemistry and molecular techniques and cytopreparation. New features incorporated throughout the book include new images, how-to illustrations for manual techniques, troubleshooting aids, and additional special staining procedures.