Advances And Challenges Of Rnai Based Technologies For Plants

Plants are vulnerable to pathogens including fungi, bacteria, and viruses, which cause critical problems and deficits. Crop protection by plant breeding delivers a promising solution with no obvious effect on human health or the local ecosystem. Crop improvement has been the most powerful approach for producing unique crop cultivars since domestication occurred, making possible the main innovations in feeding the globe and community development. Genome editing is one of the genetic devices that can be implemented, and disease resistance is frequently cited as the most encouraging application of CRISPR/Cas9 technology in agriculture. Nanobiotechnology has harnessed the power of genome editing to develop agricultural crops. Nanosized DNA or RNA nanotechnology approaches could contribute to raising the stability and performance of CRISPR guide RNAs. This book brings together the latest research in these areas. CRISPR and RNAi Systems: Nanobiotechnology Approaches to Plant Breeding and Protection presents a complete understanding of the RNAi and CRISPR/Cas9 techniques for controlling mycotoxins, fighting plant nematodes, and detecting plant pathogens. CRISPR/Cas genome editing enables efficient targeted modification in most crops, thus promising to accelerate crop improvement. CRISPR/Cas9 can be used for management of plant insects, and various plant pathogens. The book is an important reference source for both plant scientists and environmental scientists who want to understand how nano biotechnologically based approaches are being used to create more efficient plant protection and plant breeding systems. Shows how nanotechnology is being used as the basis for new solutions for more efficient plant breeding and plant protection Outlines the major techniques and applications of both CRISPR and RNAi technologies Assesses the major challenges of escalating these technologies on a mass scale

High-throughput technologies are those that allow the generation of large-scale data related to omic analysis (such as genomics, transcriptomics, proteomics, phenomics, and metabolomics). Currently, the development and use of these technologies has gone hand-in-hand with the development of bioinformatics tools, which have allowed the integration of omics-based data to understand crucial knowledge about the molecular systems that underlie the various functions of the plants. In recent years, there has been a huge development in the field of high-throughput biology, which is attributed to an era of tech startups. Genomics studies in plants, for instance, have benefited from advances in high-throughput technologies, such as high-throughput genotyping, which have allowed us to investigate the regulation and function of thousands of genes and genomic regions involved in a novel adaptation. In fact, with current high-throughput technologies, it is possible to recover substantial portions of the plant diversity, which is a key input for genetic improvement programs, food security and conservation programs. The integration of multilevel biological data, involving investigations at the molecular level (in genomics, transcriptomics, proteomics, and metabolomics, among others), have allowed more comprehensive studies, which broadens the opportunities to clarify key molecular aspects involved in the physiological responses to abiotic stresses.

Genetically engineered (GE) crops were first introduced commercially in the 1990s. After two decades of production, some groups and individuals remain critical of the technology based on their concerns about possible adverse effects on human health, the environment, and ethical considerations. At the same time, others are concerned that the technology is not reaching its potential to improve human health and the environment because of stringent regulations and reduced public funding to develop products offering more benefits to society. While the debate about these and other questions related to the genetic engineering techniques of the first 20 years goes on, emerging genetic-engineering technologies are adding new complexities to the conversation. Genetically Engineered Crops builds on previous related Academies reports published between 1987 and 2010 by undertaking a retrospective examination of the purported positive and adverse effects of GE crops and to anticipate what emerging genetic-engineering technologies hold for the future. This report indicates where there are uncertainties about the economic, agronomic, health, safety, or other impacts of GE crops and food, and makes recommendations to fill gaps in safety assessments, increase regulatory clarity, and improve innovations in and access to GE technology.

Several of the 17 papers in this volume represent diverse strategies for improving sustainability in crop production systems. The maintenance of soil quality and the reclamation of marginal soils, improving tolerance to saline irrigation water, biodegradable alternatives to black plastic mulch, use of natural plant extracts against bacterial disease, and development of cultivars resistant to herbivorous arthropods address urgent priorities in sustainable systems. Two papers examine the driving forces and effects of adopting innovative agricultural technologies in food value chains in underdeveloped regions of the world, and identification of new Asian vegetable crop species for European environments and markets. Three papers reported on managing fruit set and ripening in important fruit crop species like orange, apple, and plum. Postharvest techniques to reduce disease and maintain fruit nutraceutical content were reported in separate papers. Classification techniques, conservation and utilization of unique plant species, and in vitro propagation techniques of species with potential horticultural value were described in four papers.

This volume of Advances in Insect Physiology contains comprehensive interdisciplinary reviews on basic and practical aspects relevant to Insect Midgut and Insecticidal Proteins. Contains important, comprehensive and in-depth reviews An essential reference source for invertebrate physiologists and neurobiologists, entomologists, zoologists, and insect biochemists First published in 1963, this serial is ranked second in the highly competitive ISI category of Entomology

RNA interference (RNAi) is a widely used technology for gene silencing and has become a key tool in a myriad of research and lead discoveries. In recent years, the mechanism of RNAi agents has been well investigated, and the technique has been optimized for better effectiveness and safety. On the other hand, the clustered regularly interspaced short palindromic repeats (CRISPR)-associated Cas9/gRNA system is a recent, novel, targeted genome-editing technique derived from the bacterial immune system. Recent advances in gene-editing research and technologies have enabled the CRISPR Cas9 system to become a popular tool for sequence-specific gene editing to correct and modify eukaryotic systems. In this book, we will focus on the mechanisms, applications, regulations (their pros and cons), and various ways in which RNAi-based methods and CRIPSR-Cas9 technology have stimulated the modulation of gene expression, thereby making them a promising therapeutic tool to treat and prevent complex diseases and disorders.