Applied Biophysics

This book presents the fundamentals of molecular biophysics, and highlights the connection between molecules and biological phenomena, making it an important text across a variety of science disciplines. The topics covered in the book include: Phase transitions that occur in biosystems (protein crystallisation, globule-coil transition etc) Liquid crystallinity as an example of the delicate range of partially ordered phases found with biological molecules How molecules move and propel themselves at the cellular level The general features of self-assembly with examples from proteins The phase behaviour of DNA The physical toolbox presented within this text will form a basis for students to enter into a wide range of pure and applied bioengineering fields in medical, food and pharmaceutical areas.

Applied Biophysics for Drug Discovery is a guide to new techniques and approaches to identifying and characterizing small molecules in early drug discovery. Biophysical methods are reasserting their utility in drug discovery and through a combination of the rise of fragment-based drug discovery and an increased focus on more nuanced characterisation of small molecule binding, these methods are playing an increasing role in discovery campaigns. This text emphasizes practical considerations for selecting and deploying core biophysical method, including but not limited to ITC, SPR, and both ligand-detected and protein-detected NMR. Topics covered include: • Design considerations in biophysical-based lead screening • Thermodynamic characterization of protein-compound interactions • Characterizing targets and screening reagents with HDX-MS • Microscale thermophoresis methods (MST) • Screening with Weak Affinity Chromatography • Methods to assess compound residence time • 1D-NMR methods for hit identification • Protein-based NMR methods for SAR development • Industry case studies integrating multiple biophysical methods This text is ideal for academic investigators and industry scientists planning hit characterization campaigns or designing and optimizing screening strategies.

Applied Biophysics for Drug Discovery is a guide to new techniques and approaches to identifying and characterizing small molecules in early drug discovery. Biophysical methods are reasserting their utility in drug discovery and through a combination of the rise of fragment-based drug discovery and an increased focus on more nuanced characterisation of small molecule binding, these methods are playing an increasing role in discovery campaigns. This text emphasizes practical considerations for selecting and deploying core biophysical method, including but not limited to ITC, SPR, and both ligand-detected and protein-detected NMR. Topics covered include: • Design considerations in biophysical-based lead screening • Thermodynamic characterization of protein-compound interactions • Characterizing targets and screening reagents with HDX-MS • Microscale thermophoresis methods (MST) • Screening with Weak Affinity Chromatography • Methods to assess compound residence time • 1D-NMR methods for hit identification • Protein-based NMR methods for SAR development • Industry case studies integrating multiple biophysical methods This text is ideal for academic investigators and industry scientists planning hit characterization campaigns or designing and optimizing screening strategies.

This book provides a detailed review of the modern theories dealing with the structure and properties of water. It also presents an analysis of the research on the effect of activated water on biological systems such as animals, microorganisms, and plants. The results of experiments on the influence of activated water on “pure” microbiological cultures and their natural associations are described, the studies being carried out under both aerobic and anaerobic conditions. The results demonstrate a significant influence of activated water on higher plants (vegetable crops), sterile plants, and callus tissues. It is shown that the activation of water under definite conditions gives rise to the appearance of very strong bactericidal properties: activated water inhibits the development of pathogenic microbiological cultures by tens and hundreds of times more strongly, and can be used for sterilization. In addition, a potent positive effect of activated water on the prevention and treatment of cancer in mice has been observed, and its efficacy compared to that of chemotherapy is discussed in the book. The information provided in this book is supported by intensive experimental data and developed theories. The research programs were conducted at the authors' laboratories in Ukraine and Russia as well as at research facilities located in the USA. Contents:Introduction to the Theory of Water Memory and General Principles of Water ActivationMolecular Resonance Effect Technology as the Basic Method for Activation of Liquid SubstancesStudy of the Physical Properties of MRET Activated WaterInfluence of MRET Activated Water on the Growth of Higher PlantsEffects of MRET Activated Water on Microbial Culture and Natural Microbial AssociationsExamination of the Influence of MRET Activated Water on Prophylaxis and Treatment of OncologyEffect of MRET Activated Water on Staphylococcal Infection in vivo in Animal Model (on the Cells of Immune System) and in vitro on the Culture of Staphylococcus aureus Wood-46The Possible Mechanisms of Effects of Activated Water on Biological SystemsConclusions and Recommendations Readership: Biophysicists; physicists; medical doctors; researchers in molecular physics, hydrodynamics, optics, electrodynamics, condensed matter physics, microbiology, epidemiology and agriculture. Key Features:Presents the results of complex experimental and theoretical studies of the characteristics of activated water obtained under a controlled action of the specific non-ionizing low-frequency electromagnetic emission on ordinary waterProvides a comprehensive overview of the authors' work that includes innovative discoveries related to the effect of subtle, low-frequency, random magnetic fields on the molecular structure and physical properties of waterGives the results of the theoretical analysis of a possible mechanism of water memory and methods of its stimulationKeywords:Activated Water;Water Memory;Biophysics;Bioengineering;Biotechnology;Treatment in Oncology;Inhibition of Pathogenic Culture Growth

Biochemistry, Biophysics, and Molecular Chemistry: Applied Research and Interactions provides the background needed in biophysics and molecular chemistry and offers a great deal of advanced biophysical knowledge. It emphasizes the growing interrelatedness of molecular chemistry and biochemistry, and acquaints one with experimental methods of both disciplines. This book addresses some of the enormous advances in biochemistry, particularly in the areas of structural biology and bioinformatics, by providing a solid biochemical foundation that is rooted in chemistry. Topics include scientific integrity and ethics in the field; clinical translational research in cancer, diabetes, and cardiovascular disease; emerging drugs to treat neurodegenerative diseases; swine, avian, and human flu; the use of big data in artificial knowledge in the field; bioinformatic insights on molecular chemistry; and much more.

Molecular biophysics is a rapidly growing field of research that plays an important role in elucidating the mysteries of life's molecules and their assemblies, as well as the relationship between their structure and function. Introduction to Molecular Biophysics fills an existing gap in the literature on this subject by providing the reader with th

In the first volume, Fundamental Concepts in Biophysics, the authors lay down a foundation for biophysics study. Rajiv Singh opens the book by pointing to the central importance of “Mathematical Methods in Biophysics”. William Fink follows with a discussion on “Quantum Mechanics Basic to Biophysical Methods”. Together, these two chapters establish some of the principles of mathematical physics underlying many biophysics techniques. Because computer modeling forms an intricate part of biophysics research, Subhadip Raychaudhuri and colleagues introduce the use of computer modeling in “Computational Modeling of Receptor–Ligand Binding and Cellular Signaling Processes”. Yin Yeh and coworkers bring to the reader’s attention the physical basis underlying the common use of fluorescence spectroscopy in biomedical research in their chapter “Fluorescence Spectroscopy”. Electrophysiologists have also applied biophysics techniques in the study of membrane proteins, and Tsung-Yu Chen et al. explore stochastic processes of ion transport in their “Electrophysiological Measurements of Membrane Proteins”. Michael Saxton takes up a key biophysics question about particle distribution and behavior in systems with spatial or temporal inhomogeneity in his chapter “Single–Particle Tracking”. Finally, in “NMR Measurement of Biomolecule Diffusion”, Thomas Jue explains how magnetic resonance techniques can map biomolecule diffusion in the cell to a theory of respiratory control. This book thus launches the Handbook of Modern Biophysics series and sets up for the reader some of the fundamental concepts underpinning the biophysics issues to be presented in future volumes.