Applied Molecular Genetics

This text explains the key biochemical and cell biological principles behind some of today's most commonly used applications of molecular genetics, using clear terms and well-illustrated flow schemes. The book is divided into several sections and moves from basic to advanced topics while providing a concise overview of fundamental concepts in modern biotechnology. Each chapter concludes with a Laboratory Practicum describing a hypothetical research objective and the sequence of steps that are most often used to investigate biological questions using molecular genetic methods. In addition, the book provides informative summaries of the latest advances in molecular genetics, using attractive illustrations and a comprehensive reference list. This text also introduces the use of Internet resources through the World Wide Web as a powerful new tool in molecular genetic research. Seven appendices are included in the book, providing a convenient information resource for properties of nucleic acids, protein and restriction enzymes, a description of common E. coli genetic markers and gel electrophoresis parameters, as well as a list of useful Internet address sites.

Applied Molecular Biotechnology: The Next Generation of Genetic Engineering explains state-of-the-art advances in the rapidly developing area of molecular biotechnology, the technology of the new millennium. Comprised of chapters authored by leading experts in their respective fields, this authoritative reference text:Highlights the latest omics-ba

The interactions of fungi with mankind are both beneficial and harmful and are deeply rooted in the history of human society and agriculture. This book highlights the ways in which fungal recombinant DNA technology is being used in species of economic importance.

The filamentous fungi are perhaps unique in the diversity of their metabolic activities. This includes biosynthetic as well as degradative activities, many is to provide an of which are of industrial interest. The objective of this text up-to-date and broad review which emphasizes the genetic and molecular biological contribution in the field of fungal biotechnology. This text begins with an overview of the tools and methodologies involved which, to a large extent, have been developed in the model filamentous fungus Aspergillus nidulans and subsequently have been extended to commercially important fungi. This is followed by a chapter which provides a compilation of genes isolated from commercial fungi and their present status with respect to structure, function and regulation. Chapters 3 and 4 highlight the degradative powers of filamentous fungi. First, a discussion of what is known regarding the molecular genetics of fungi and the genes and enzymes involved in the beverage and food industries. This has an oriental flavour, reflecting the tremendous importance of fungi in traditional Chinese and Japanese food production. An account of lignocellulose degradation by filamentous fungi follows, illustrating the potential of fungi to utilize this substance as a renewable energy source. The ability of fungi to produce high-value foreign proteins is reviewed in chapters 5 and 6. Chymosin production, in particular, represents a good example of high-level yields being obtained, such as to warrant commercial production.