Aqueous Two Phase Systems For Bioprocess Development For The Recovery Of Biological Products

This comprehensive and unique text presents a full overview of downstream processing useful for those new to the concept as well as professionals with experience in the area. The history and theoretical principles of Aqueous Two-Phase Systems (ATPS) are covered in depth. Information on ATPS characterization and application is included, and ATPS equilibria and system parameters that have significant effect on partition behavior are studied. Aqueous Two-Phase Systems for Bioprocess Development for the Recovery of Biological Products addresses specific applications of ATPS for the recovery and partial purification of high molecular weight compounds such as proteins, nucleic acids and polysaccharides, particulate bioproducts such as cells and organelles and low molecular weight compounds. Non-conventional strategies involving ATPS such as affinity systems, continuous liquid-liquid fractionation stages and the recovery from plant extracts are presented. Economic analysis of the application of ATPS in comparison to other fractionation techniques, particularly liquid chromatography, is considered, as are opportunity and current trends in the ATPS research area. Each chapter utilizes the contributors' experimental expertise in traditional and non-conventional ATPS strategies, as well as analysis of areas of opportunity and perspectives on the development and future applications of ATPS in both the lab and larger scale operations. The result is a thorough and singular overview of ATPS which has not been matched by any other text on the market.

Application of Process Intensification (PI) presents a set of radically innovative principles in process and equipment design, which can bring significant benefits in terms of process efficiency, capital and operating expenses, quality, process safety, and sustainability. Typical approaches in bioprocess intensification are the reduction of the number of production steps, continuous processing, integrated processes, and alternative energy inputs.

Liquid Phase Extraction thoroughly presents both existing and new techniques in liquid phase extraction. It not only provides all information laboratory scientists need for choosing and utilizing suitable sample preparation procedures for any kind of sample, but also showcases the contemporary uses of sample preparation techniques in the most important industrial and academic project environments, including countercurrent chromatography, pressurized-liquid extraction, single-drop Microextraction, and more. Written by recognized experts in their respective fields, it serves as a one-stop reference for those who need to know which technique to choose for liquid phase extraction. Used in conjunction with a similar release, Solid Phase Extraction, it allows users to master this crucial aspect of sample preparation. Defines the current state-of-the-art in extraction techniques and the methods and procedures for implementing them in laboratory practice Includes extensive referencing that facilitates the identification of key information Aimed at both entry-level scientists and those who want to explore new techniques and methods

Biotechnology is a diverse, complex and rapidly evolving field. Students and experienced researchers alike face the challenges of staying on top of developments in their field of specialty and maintaining a broader overview of the field as a whole. Volumes containing competent reviews on a diverse range of topics in the field fulfill the dual role of broadening and updating biotechnologists’ knowledge. The current volume is an excellent example of such a book. The topics covered range from classical issues in biotechnology - such as, recent advances in all-protein chromophore technology- to topics that are focused on coencapsulation of hepatocytes and bone marrow cell. The information presented in this book will therefore will be of great value to both experienced biotechnologists and biotechnologists in training. Includes over 80 illustrations and photographs Discusses the recent developments in biodegradable synthetic polymers Offers a detailed discussion on emerging options in protein bioseparation

Food Engineering Handbook, Two-Volume Set provides a stimulating and up-to-date review of food engineering phenomena. It also addresses the basic and applied principles of food engineering methods used in food processing operations around the world. Combining theory with a practical, hands-on approach, this set examines the thermophysical properties and modeling of selected processes such as chilling, freezing, and dehydration, and covers the key aspects of food engineering, from mass and heat transfer to steam and boilers, heat exchangers, diffusion, and absorption. Comprised of Food Engineering Handbook: Food Engineering Fundamentals and Food Engineering Handbook: Food Process Engineering, this comprehensive resource: Explains the interactions between different food constituents that might lead to changes in food properties Describes the characterization of the heating behavior of foods, their heat transfer, heat exchangers, and the equipment used in each food engineering method Discusses rheology, fluid flow, evaporation, distillation, size reduction, mixing, emulsion, and encapsulation Provides case studies of solid–liquid and supercritical fluid extraction and food behaviors Explores fermentation, enzymes, fluidized-bed drying, and more Presenting cutting-edge information on new and emerging food engineering processes, Food Engineering Handbook, Two-Volume Set offers a complete reference on the fundamental concepts, modeling, quality, safety, and technologies associated with food engineering and processing operations today.

The phase separation of incompatible liquids has been a topic of significant importance in chemical and industrial engineering for many years. Well-understood examples of this phenomenon include the phase separation of oil with water and the phase separation of non-polar organic solvents with water. Similar behavior is observed when aqueous solutions of two or more incompatible polymers or polymers and salts are mixed. In these mixtures (referred to as aqueous two-phase systems), the separated phases are composed mostly of water. Aqueous two-phase systems have been used extensively for the extraction of high-value biological products from mixtures of biological materials. In recent years, aqueous two-phase systems have also found increased use as materials for streamlining and improving the capabilities of cell and molecular assays, and for the design of advanced cell culture systems. Similar behavior of biological materials in living systems has also been observed, with emerging roles in cell physiology.

Food Engineering Handbook: Food Process Engineering addresses the basic and applied principles of food engineering methods used in food processing operations around the world. Combining theory with a practical, hands-on approach, this book examines the thermophysical properties and modeling of selected processes such as chilling, freezing, and dehy