Assessing The Functional Structure Of Molecular Transporters By Epr Spectroscopy

In his thesis, Matthias Junk takes an innovative approach to assess the local structure and dynamics of biological and synthetic amphiphilic macromolecules capable of transporting small molecules. Replacing the latter with stable radicals, he uses state-of-the-art electron paramagnetic resonance (EPR) spectroscopy to describe the highly relevant transport function from the viewpoint of the guest molecules. Such, he demonstrates that the functional structure of human serum albumin in solution significantly differs from its crystal structure – a consequence of the protein’s adaptability to host various endogenous compounds and drug molecules. Further, he shows that the thermal collapse of thermoresponsive hydrogels and dendronized polymers leads to static and dynamic heterogeneities on the nanoscale. These heterogeneities bear consequences for the material’s hosting properties and enable unforeseen complex catalytic functionalities.

In his thesis, Matthias Junk takes an innovative approach to assess the local structure and dynamics of biological and synthetic amphiphilic macromolecules capable of transporting small molecules. Replacing the latter with stable radicals, he uses state-of-the-art electron paramagnetic resonance (EPR) spectroscopy to describe the highly relevant transport function from the viewpoint of the guest molecules. Such, he demonstrates that the functional structure of human serum albumin in solution significantly differs from its crystal structure – a consequence of the protein’s adaptability to host various endogenous compounds and drug molecules. Further, he shows that the thermal collapse of thermoresponsive hydrogels and dendronized polymers leads to static and dynamic heterogeneities on the nanoscale. These heterogeneities bear consequences for the material’s hosting properties and enable unforeseen complex catalytic functionalities.

Amino Acids, Peptides and Proteins comprises a comprehensive and critical review of significant developments at the biology and chemistry interface. Compiled by leading researchers in their subject, this volume incorporates current trends and emerging areas in topics such as magnetic resonance studies of membrane active peptides, proteins and peptides for the diagnosis and therapy of Leishmania donovani parasite infections and advances in the design of ligands interacting with proteases causing infectious respiratory syndrome. Appealing broadly to researchers in academia and industry, it will be of great benefit to any researcher wanting a succinct reference on developments in this area now and looking to the future.

This work will serve as a definitive overview of the field of computational simulation as applied to analytical chemistry and biology, drawing on recent advances as well as describing essential, established theory for graduates and postgraduate researchers.

This book shows how the fundamentals of electron paramagnetic resonance (EPR) spectroscopy are practically implemented and illustrates the diversity of current applications. The technique is used at various levels, and applications are presented in order of increasing difficulty, with reference to theoretically obtained results. This book features a diverse array of application examples, from fields such as ionizing radiation dosimetry, neurodegenerative diseases, structural transitions in proteins, and the origins of terrestrial life. The final chapter of this book highlights the principles and applications of the technique of ferromagnetic resonance spectroscopy, followed by a brief introduction to advanced EPR techniques such as electron spin echo envelope modulation (ESEEM), hyperfine sub-level correlation (HYSCORE), pulsed electron-electron double resonance (PELDOR), and continuous wave electron nuclear double resonance (ENDOR) experiments.

This book provides the most comprehensive and consistent survey of the field of IC design for Biological Sensing and Processing. The authors describe a multitude of applications that require custom CMOS IC design and highlight the techniques in analog and mixed-signal circuit design that potentially can cross boundaries and benefit the very wide community of bio-medical engineers.

The combination of its unique morphology, physical properties, cost effectiveness and environmental friendliness make natural rubber an appealing constituent for many materials and applications. This comprehensive two volume set covers the synthesis, characterization and applications of natural rubber based blends, interpenetrating polymer networks, composites and nanocomposites. Volume 1 covers different types of natural rubber-based blends and IPNs as well as manufacturing methods, thermo mechanical characterization techniques, life cycle analysis and their applications. Volume 2 focuses on natural rubber-based composites and Nanocomposites including the different types of fillers, the filler-matrix reinforcement mechanisms, manufacturing techniques, and applications. This is the first book to consolidate the current state of the art information on natural rubber based materials with contributions from established international experts in the field. The book provides a "one stop" reference resource for professionals, researchers, industrial practitioners, graduate students, and senior undergraduates in the fields of polymer science and engineering, materials science, surface science, bioengineering and chemical engineering.

A Structure-Function Toolbox for Membrane Transporter and Channels, Volume 594, a new release in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. New chapters in the updated serial include Cryo-EM on membrane proteins embedded in nanodics, Solid-Supported membrane-based electrophysiology on membrane transporters and channels, Saposin-lipoprotein scaffolds for structure determination of membrane transporters and channels, Single-molecule FRET on transporters, Dynamics of channels and transporters investigated by NMR, Structure-function studies on channels and transporters, and a section on MemStar, a new GFP-based expression and purification system for transporters and channels. Continues the legacy of this premier serial with quality chapters authored by leaders in the field Covers membrane transporter and channels