Atlas Of Zebrafish Development

Zebrafish are widely considered an excellent model system for vertebrate development. The embryo is transparent, thereby enabling visualization and use of labelling and transgenic approaches. Moreover, because of the ease of inducing new mutations in zebrafish and similarity with the human genome, this organism may be used effectively for disease studies. For example, mutant zebrafish are being utilized for testing drugs that will combat a range of human diseases, from Alzheimer's and cancer to kidney failure and congenital heart disease. For the first time, this atlas provides the research community with a complete reference for zebrafish anatomy spanning the early embryo all the way to adulthood. The authors employ the technique of optical projection tomography (OPT), and offer a series of sections in multiple planes from each sample. The contents are organized by developmental stages, with over 200 images that contain annotations describing anatomical structures relevant to development. In addition, chapters feature explanatory text that highlights major developments in the zebrafish during each stage. Provides the first comprehensive anatomical resource that covers all regions of zebrafish anatomy from the larval period to adulthood The over 200 images include explanatory notes Each chapter contains a concise description of key anatomical features that factor in zebrafish development Despite many years of use as a model system, until now there has never been a guide to zebrafish at the larval stage The book's website contains a database of over 10k sections from different regions as well as 3D images that are interactive

Zebrafish are widely considered an excellent modelsystem for vertebrate development. The embryo is transparent, thereby enabling visualization and use of labelling and transgenic approaches. Moreover, because of the ease of inducing new mutations in zebrafish and similarity with the human genome, this organism may be used effectively for disease studies. For example, mutant zebrafish are being utilized for testing drugs that will combat a range of human diseases, from Alzheimer's and cancer to kidney failure and congenital heart disease. For the first time, this atlas provides the research community with a complete reference for zebrafish anatomy spanning the early embryo all the way to adulthood. The authors employ the technique of optical projection tomography (OPT), and offer a series of sections in multiple planes from each sample. The contents are organized by developmental stages, with over 200 images that contain annotations describing anatomical structures relevant to development. In addition, chapters feature explanatory text that highlights major developments in the zebrafish during each stage. Provides the first comprehensive anatomical resource that covers all regions of zebrafish anatomy from the larval period to adulthoodThe over 200 images include explanatory notesEach chapter contains a concise description of key anatomical features that factor in zebrafish developmentDespite many years of use as a model system, until now there has never been a guide to zebrafish at the larval stageThe book's website contains a database of over 10k sections from different regions as well as 3D images that are interactive"

Atlas of Early Zebrafish Brain Development: A Tool for Molecular Neurogenetics, Second Edition, remains the only neuroanatomical expression atlas of important genetic and immunohistochemical markers of this vertebrate model system. It represents a key reference and interpretation matrix for analyzing expression domains of genes involved in Zebrafish brain development and neurogenesis, and serves as a continuing milestone in this research area. This updated volume provides in-situ hybridized and immunostained preparations of complete series of brain sections, revealing markers of the fundamental stages in the life history of neuronal cells in very high quality preparations and photographic plates. Specific additions to this edition include documentation on the distribution of neurons expressing GABA, dopamine and serotonin, material on the basal ganglia, hypothalamus, and the caudal, segmented part of the diencephalon, new theories on the early organization of the telencephalon and thalamus, and integration of a comparative perspective on the mid- and hindbrain. Documentation on the distribution of neurons expressing GABA, dopamine and serotonin Material on the basal ganglia, hypothalamus, and the caudal, segmented part of the diencephalon New theories about the early organization of the telencephalon and thalamus Integration of a comparative perspective on the mid- and hindbrain

The zebrafish (Danio rerio) is a valuable and common model for researchers working in the fields of genetics, oncology and developmental sciences. This full-color atlas will aid experimental design and interpretation in these areas by providing a fundamental understanding of zebrafish anatomy. Over 150 photomicrographs are included and can be used for direct comparison with histological slides, allowing quick and accurate identification of the anatomic structures of interest. Hematoxylin and eosin stained longitudinal and transverse sections demonstrate gross anatomic relationships and illustrate the microscopic anatomy of major organs. Unlike much of the current literature, this book is focused exclusively on the zebrafish, eliminating the need for researchers to exclude structures that are only found in other fish.

"The book introduces the reader to the field and shows principles of vertebrate brain development and neurogenesis. It will also be of great value to investigators of other model organisms such as frog, chick, and mouse."--BOOK JACKET.

gene-expression patterns in the zebrafish correspond well The zebrafish belongs to the teleostean family of with the neuromeric model of Puelles and Rubenstein cyprinids (minnows), which includes other neurobiologi­ (1993) is very encouraging for further research in this cally well investigated species such as goldfish (Carassius model system, which holds the promise of revealing fun­ auratus) and carp (e. g. Cyprinus carpio). damental neurogenetic patterns and mechanisms in ver­ In a recent taxonomic revision (Barman, 1991), all spe­ tebrates. cies of the genus Brachydanio were reassigned to the genus Danio. Thus, the current species designation of the zebrafish is Danio (formerly Brachydanio) rerio (Hamil­ ton-Buchanan, 1822). As a cyprinid, the zebrafish belongs Generation of zebrafish mutants via saturation mutagenesis to the large group of ostariophysan teleosts (6000 species, The goal of saturation mutagenesis is to identify most - if Lauder and Liem, 1983). Most ostariophysans are charac­ not all- genes affecting early animal development and to terized by a sophisticated apparatus - the Weberian ossi­ understand their hierarchical, regulative role during de­ des - to transmit sound from the swim bladder to the velopment (for reviews, see: Mullins and Ntisslein-Vol­ inner ear. The ostariophysans belong to the most derived hard, 1993; Solnica-Krezel et aI. , 1994). This approach has group of teleosts, the euteleosts (17000 species), which recently been applied to the zebrafish system, and the re­ have three more basic teleostean outgroups, the oste­ sults are impressive.

The zebrafish has become one of the most important model organisms to study biological processes within a living body. As a vertebrate that has many of the strengths of invertebrate model systems, it offers numerous advantages to researchers interested in many aspects of embryonic development, physiology and disease. This book not only provides a complete set of instructions that will allow researchers to establish the zebrafish in their laboratory. It also gives a broad overview of commonly used methods and a comprehensive collection of protocols describing the most powerful techniques.

This book elucidates the tissue structure and cell composition of the organs of zebrafish at the microscopic, ultrastructural and molecular levels. The distribution of important macromolecular substances is shown and the morphological relationship between different components is analyzed. The book is divided into 15 chapters and contains more than 700 structural photos, all of which are original experimental pictures of the research group. It shows the histological panorama of the whole zebrafish both in cross and longitudinal sections and covers and interprets the tissues and organs of zebrafish in detail, including oropharynx, taste buds, pharyngeal teeth, liver, etc. A brief text description of the structure and function meaning is available for every picture to facilitate the audience understanding the theoretical knowledge more vivid and concrete. In addition, the 3D reconstruction of the main organs of zebrafish is completed by computer-aided technology, and the three-dimensional morphology of the organs is displayed in an intuitive form. This book provides a reference for postgraduates and researchers in anatomy, biology, animal medicine, animal science, aquaculture, developmental biology, medicine, and experimental animals.