Basic Serological Testing

This book discusses in detail various serological tests that are used to identify medical conditions and diseases, from a general overview of each test to the equipment and steps needed to carry them out. The book is aimed specifically at immunology students and professionals who may occasionally need to use these tests, and thus lack training and experience in performing them. The book provides a brief overview of the immune system, including antibodies, antigens, and their interactions. The bulk of the book is comprised of 16 chapters that each explain different serological tests. These chapters start with a general introduction of the test or disease being detected, followed by the test principle, reagents required for the test, procedures and steps to perform the test, and, finally, result interpretation. Both test principles and result interpretation segments include illustrations to aid comprehension. In addition, the book also enables the reader to distinguish between positive and negative results in serological testing.

This is a concise, highly accessible introduction to medical virology, incorporating essential basic principles as well as a systematic review of viruses and viral diseases. It pays particular attention to developments in anti-viral therapy that are becoming increasingly effective in modern medicine. It is an ideal textbook for the information-overloaded student and an invaluable everyday companion for the busy professional who needs a good understanding of the current state of medical virology. In keeping with the highly successful format of other Illustrated Colour Texts, it presents the subject as a series of succinct 2 page ‘learning units’, using a superb collection of clear illustrations and clinical photographs, concise yet comprehensive text and key point boxes to aid quick access to information and examination preparation. So whether you are a medical student, junior doctor, medical scientist, trainee in infectious diseases or student on another allied medical course, this book is here to make your life easier! It will also provide a very solid foundation for any who plan to delve deeper into this fascinating field. Part of the popular Illustrated Colour Text series Information presented in double page spreads for easy learning Highly illustrated with both full colour graphics and clinical photographs Each spread includes a key point box for exam preparation

Testing and diagnosis of hepatitis B (HBV) and C (HCV) infection is the gateway for access to both prevention and treatment services, and is a crucial component of an effective response to the hepatitis epidemic. Early identification of persons with chronic HBV or HCV infection enables them to receive the necessary care and treatment to prevent or delay progression of liver disease. Testing also provides an opportunity to link people to interventions to reduce transmission, through counselling on risk behaviors and provision of prevention commodities (such as sterile needles and syringes) and hepatitis B vaccination. These are the first WHO guidelines on testing for chronic HBV and HCV infection and complement published guidance by WHO on the prevention, care and treatment of chronic hepatitis C and hepatitis B infection. These guidelines outline the public health approach to strengthening and expanding current testing practices for HBV and HCV, and are intended for use across age groups and populations.

The Virology Methods Manual is a comprehensive source of methods for the study, manipulation, and detection of viruses. Edited by Brian Mahy and Hillar Kangro, this work describes the most up-to-date, definitive techniques, provided by experts in each area, and presented with easy-to-use, step-by-step protocols. This new manual will satisfy the needs of virologists and all those working with viruses who need a practical guide to methods that work! Provides up-to-date techniques by experts worldwide Presents common, step-by-step protocols in an attractive, easy-to-use fashion Contains useful appendices including virus taxonomy, metabolic inhibitors, and Bio-safety in the virology laboratory

This open access book presents the first step towards building socio-life science, a field of science investigating humans in such a way that both social and life-scientific factors are integrated. Because humans are both living and social creatures, a human action can never be understood fully without knowing both the biological traits of a person and the social scientific environments in which he exists. With this consideration, the editors of this book have initiated a research project promoting a deeper and more integrated understanding of human behavior and human health. This book aims to show what can, and could be, achieved through our interdisciplinary project. One important product is the newly formed three-party collaboration between Pasteur Institut, Kyoto University, and the Research Institute of Economy, Trade and Industry. Covering many different fields, including medicine, epidemiology, anthropology, economics, sociology, demography, geography, and policy, researchers in these institutes, and many others, present their studies on the COVID-19 pandemic. Although based on different methodologies, the studies show the importance of behavioral change and governmental policy in the fight against a huge pandemic. The book explains the unique genome cohort-panel data that the project builds to study social and life scientific aspects of humans.

Western Carolina University, Cullowhee, North Carolina. Introduction to clinical immunology, for second and amp; fourthyear level clinical laboratory science students. Combines essential theoretical principles with commonly used serological techniques. Chapters include objectives, outlines, and glossary.

Immunology and Serology are two major science fields. Immunology is defined as the study of the molecules, cells, organs, and systems responsible for the recognition and disposal of foreign material. Immunology began as a branch of microbiology. The study of infectious disease and the body's response to them has a major role for the development of immunology. Moreover, the concept of germ theory of disease has contributed to the field of immunology. It was Edward Jenner who first studied the response of the body to foreign substances. He observed that dairy maids who had naturally contracted a mild infection called cowpox seemed to be protected against smallpox, a horribly disfiguring disease and a major killer.Serology is the diagnostic identification of antibodies in the serum and other bodily fluids. Such antibodies are typically formed in response to an infection (against a given microorganism), against other foreign proteins (in response, for example, to a mismatched blood transfusion), or to one's own proteins (in instances of autoimmune disease).Serological tests may be performed for diagnostic purposes when an infection is suspected, in rheumatic illnesses, and in many other situations, such as checking an individual's blood type. Serology blood tests help to diagnose patients with certain immune deficiencies associated with the lack of antibodies, such as X-linked agammaglobulinemia. In such cases, tests for antibodies will be consistently negative.There are several serology techniques that can be used depending on the antibodies being studied. These include: ELISA, agglutination, precipitation, complement-fixation, and fluorescent antibodies and more recently chemiluminescence. Some serological tests are not limited to blood serum, but can also be performed on other bodily fluids such as semen and saliva, and Spinal fluid (CSF) which may contain antibodies.This book starts with a small historical introduction to Immunology. The next chapters (sections 1 to 4) give examples of Serology applied to infectious diseases (HPV, Hepatitis, Malaria and Dengue). Section 5 is dedicated to the application of serology to celiac diagnosis. Section 6 shows the application of serology to other pathogen (Lyme disease, Sjögren's syndrome, Chlamydia pneumoniae, HIV, Influenza virus, Mycobacterium, Toxoplasmosis and Leprosy). Several serologic based diagnostic techniques are used and are being developed daily, making this one of the biggest fields in science research.

"Blood transfusion is a life-saving intervention that has an essential role in patient management within health care systems. All Member States of the World Health Organization (WHO) endorsed World Health Assembly resolutions WHA28.72 (1) in 1975 and WHA58.13 (2) in 2005. These commit them to the provision of adequate supplies of safe blood and blood products that are accessible to all patients who require transfusion either to save their lives or promote their continuing or improving health." --Preface.