Cell Differentiation

Recent scientific breakthroughs, celebrity patient advocates, and conflicting religious beliefs have come together to bring the state of stem cell researchâ€"specifically embryonic stem cell researchâ€"into the political crosshairs. President Bush's watershed policy statement allows federal funding for embryonic stem cell research but only on a limited number of stem cell lines. Millions of Americans could be affected by the continuing political debate among policymakers and the public. Stem Cells and the Future of Regenerative Medicine provides a deeper exploration of the biological, ethical, and funding questions prompted by the therapeutic potential of undifferentiated human cells. In terms accessible to lay readers, the book summarizes what we know about adult and embryonic stem cells and discusses how to go about the transition from mouse studies to research that has therapeutic implications for people. Perhaps most important, Stem Cells and the Future of Regenerative Medicine also provides an overview of the moral and ethical problems that arise from the use of embryonic stem cells. This timely book compares the impact of public and private research funding and discusses approaches to appropriate research oversight. Based on the insights of leading scientists, ethicists, and other authorities, the book offers authoritative recommendations regarding the use of existing stem cell lines versus new lines in research, the important role of the federal government in this field of research, and other fundamental issues.

The development of an embryo is one of the which prevents entry of other sperm, fusion of most awe inspiring biological phenomena and the two haploid nuclei occurs and within about the study of cell differentiation can be traced 30 minutes the pigmented cortex rotates with respect to the underlying cytoplasm and in so back in antiquity to Aristotle and beyond However, there are few modern sciences which doing it reveals a grey, crescent shaped area on pay more than a cursory obeisance to their the side of the egg opposite to the point of founders and few students seem very interested entry of the sperm. This is another example of in the theories of their dead predecessors. polarity developing. Soon after fertilization the Embryology, though, is that rare exception - a zygote enters a period of rapid nuclear and cell division. The result of this cleavage process is science where the problems, theories and often that the egg cytoplasm is partitioned between techniques that excite our interest today, are essentially the same as those which excited our numerous cells whose ratio of nuclear volume to cytoplasmic volume is more like that found colleagues of fifty or even a hundred years ago. in an 'average' somatic cell.

This new book presents research developments from around the globe in the field of cellular differentiation which is a concept from developmental biology describing the process by which cells acquire a 'type'. The morphology of a cell may change dramatically during differentiation, but the genetic material remains the same, with few exceptions. A cell that is able to differentiate into many cell types is known as pluripotent. These cells are called stem cells in animals and meristematic cells in higher plants. A cell that is able to differentiate into all cell types is known as totipotent. In mammals, only the zygote and early embryonic cells are totipotent, while in plants, many differentiated cells can become totipotent with simple laboratory techniques.

This book presents the current state of knowledge on the origin and differentiation of cell lines involved in the development of the vertebrate male and female gonads with particular emphasis on the mouse. It also discusses the processes leading to the testis- and ovary-specific structures and functions. The individual chapters review the origin and differentiation of the somatic cells of the genital ridges; the formation and migration of primordial germ cells in mouse and man; the gonadal supporting cell lineage and mammalian sex determination; differentiation of Sertoli and granulosa cells; mesonephric cell migration into the gonads and vascularization; origin and differentiation of androgen-producing cells in the gonads; germ cell commitment to the oogenic versus spermatogenic pathway and the role of retinoic acid; ovarian folliculogenesis; control of oocyte growth and development by intercellular communication within the follicular niche; biology of the Sertoli cell in the fetal, pubertal and adult mammalian testis; mechanisms regulating spermatogonial differentiation; stem cells in mammalian gonads; the role of microRNAs in cell differentiation during gonad development; human sex development and its disorders; as well as methods for the study of gonadal development.

This series was established to create comprehensive treatises on specific topics in developmental biology. Such volumes serve a useful role in developmental biology, which is a very diverse field that receives contributions from a wide variety of disciplines. This series is a meeting ground for the various practi tioners of this science, facilitating an integration of heterogeneous information on specific topics. Each volume is comprised of chapters selected to provide the conceptual basis for a comprehensive understanding of its topic as well as an analysis of the key experiments upon which that understanding is based. The specialist in any aspect of developmental biology should understand the experimental back ground of the specialty and be able to place that body of information in context, in order to ascertain where additional research would be fruitful. The creative process then generates new experiments. This series is intended to be a vital link in that ongoing process of learning and discovery.

This book explores the vital importance of T-cell differentiation in areas as wide-ranging as pathological analysis, drug development, and cell therapy of human T-cells. Focusing on human embryonic stem cells and human induced pluripotent stem cells, the chapters explore a variety of in vitro T-cell differentiation protocols as well as useful techniques to develop and evaluate cellular medicines. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, In Vitro Differentiation of T-Cells: Methods and Protocols serves as an ideal guide for researchers seeking to differentiate T-cells from pluripotent stem cells in order to achieve any number of significant goals.

With improved microscope and preparation techniques, studies of histo logical structures of plant organisms experienced a revival of interest at the end of the 19th century. From that time, histological data have sub stantially studies of the pioneers in botanical science. From the beginning of the 20th century, the microscope allowed research in cell structure, the general functional unit of living beings. Advances in cytology gradually influenced histology, at first, however, rather timidly. Only the new and spectacular progress in ultrastructural cytology and cytochemistry led to a great increase in modern work on the structures of vascular plants and the related ontogenical and physiological data, thanks to the use of the electron microscope and the contribution of molecular biology. Not only did new techniques lead to new approaches, but achieve ments in general biology shifted the orientation of research, linking in vestigation to the physiological aspects of cell and tissue differentiation. Among these, the demonstration of the general principles of develop ment, and the characterization of molecules common to plants and animals, which control and govern the main basic functions of cells and tissues, have widened the scope of modern research on plant struc tures. Present trends in biological research show that it is necessary to know the structures thoroughly, from the ultrastructural cytological scale to the scale of tissue and organ arrangement, even for physiological research on either cells, tissues, or whole organs. The study of growth factors, differ entiation, or organogenesis can be mentioned as an example.