Collagen From Tissue Culture To Biomaterials Tissue Engineering And Beyond

As collagen is the main structural component of body tissues, there has been much effort expended, both in the past and today, to use it in cell culture applications. Indeed, its importance is further stressed by the wide use of collagen biomaterials in tissue repair and regeneration, including the treatment of burns, chronic wounds or bone repair. This book represents a journey through recent scientific history, and describes the massive implications of the research carried out by Bornstein, Ehrmann and Gey on collagen preparations in the 1950s for cell culture and more recently, tissue engineering and personalised medicine.

The Cell-Surface Interaction, by J. S. Hayes, E. M. Czekanska and R. G. Richards. Studying Cell-Surface Interactions In Vitro: A Survey of Experimental Approaches and Techniques, by Stefanie Michaelis, Rudolf Robelek and Joachim Wegener. Harnessing Cell-Biomaterial Interactions for Osteochondral Tissue Regeneration, by Kyobum Kim, Diana M. Yoon, Antonios G. Mikos and F. Kurtis Kasper. Interaction of Cells with Decellularized Biological Materials, by Mathias Wilhelmi, Bettina Giere and Michael Harder. Evaluation of Biocompatibility Using In Vitro Methods: Interpretation and Limitations, by Arie Bruinink and Reto Luginbuehl. Artificial Scaffolds and Mesenchymal Stem Cells for Hard Tissues, by Margit Schulze and Edda Tobiasch. Bioactive Glass-Based Scaffolds for Bone Tissue Engineering, by Julia Will, Lutz-Christian Gerhardt and Aldo R. Boccaccini. Microenvironment Design for Stem Cell Fate Determination, by Tali Re’em and Smadar Cohen. Stem Cell Differentiation Depending on Different Surfaces, by Sonja Kress, Anne Neumann, Birgit Weyand and Cornelia Kasper. Designing the Biocompatibility of Biohybrids, by Frank Witte, Ivonne Bartsch and Elmar Willbold. Interaction of Cartilage and Ceramic Matrix, by K. Wiegandt, C. Goepfert, R. Pörtner and R. Janssen. Bioresorption and Degradation of Biomaterials, by Debarun Das, Ziyang Zhang, Thomas Winkler, Meenakshi Mour, Christina I. Günter, Michael M. Morlock, Hans-Günther Machens and Arndt F. Schilling.

Step-by-step, practical guidance for the acquisition, manipulation,and use of cell sources for tissue engineering Tissue engineering is a multidisciplinary field incorporatingthe principles of biology, chemistry, engineering, and medicine tocreate biological substitutes of native tissues for scientificresearch or clinical use. Specific applications of this technologyinclude studies of tissue development and function, investigatingdrug response, and tissue repair and replacement. This area israpidly becoming one of the most promising treatment options forpatients suffering from tissue failure. Written by leading experts in the field, Culture of Cellsfor Tissue Engineering offers step-by-step, practicalguidance for the acquisition, manipulation, and use of cell sourcesfor tissue engineering. It offers a unique focus on tissueengineering methods for cell sourcing and utilization, combiningtheoretical overviews and detailed procedures. Features of the text include: Easy-to-use format with a two-part organization Logically organized—part one discusses cell sourcing,preparation, and characterization and the second part examinesspecific engineered tissues Each chapter covers: structural and functional properties oftissues, methodological principles, culture, cellselection/expansion, cell modifications, cell seeding, tissueculture, analytical assays, and a detailed description ofrepresentative studies End-of-chapter features include useful listings of sources forreagents, materials, and supplies, with the contact details of thesuppliers listed at the end of the book A section of elegant color plates to back up the figures in thechapters Culture of Cells for Tissue Engineering givesnovice and seasoned researchers in tissue engineering an invaluableresource. In addition, the text is suitable for professionals inrelated research, particularly in those areas where cell and tissueculture is a new or emerging tool.

In recent years, the field of tissue engineering has begun, in part, to c- lesce around the important clinical goal of developing substitutes or repla- ments for defective tissues or organs. These efforts are focused on many tissues including skin, cartilage, liver, pancreas, bone, blood, muscle, the vascu- ture, and nerves. There is a staggering medical need for new and effective treatments for acquired as well as inherited defects of organs/tissues. Tissue engineering is at the interface of the life sciences, engineering, and clinical medicine and so draws upon advances in cell and molecular biology, mate- als sciences, and surgery, as well as chemical and mechanical engineering. Such an interdisciplinary field requires a broad knowledge base as well as the use of a wide assortment of methods and approaches. It is hoped that by bringing together these protocols, this book will help to form connections - tween the different disciplines and further stimulate the synergism underlying the foundation of the tissue engineering field.

The increased use of biodegradable synthetic or natural scaffolds combined with cells and/or biological molecules, in order to create functional replacement tissue in a damaged tissue site, has led to the need for the development of ‘best practice’ methods in the area of tissue engineering to help ensure the creation of safe, high quality products. Standardisation in cell and tissue engineering introduces concepts and current practice in the field of cell and tissue engineering to a wide audience and aims to provide awareness of the importance of standardisation in this area while suggesting directions for further investigation. Part one provides an overview of methods for cell and tissue engineering and includes chapters on the fundamentals of cell and matrix biology for tissue engineering, 3D collagen biomatrix development, and control and vascularisation of tissue-engineered constructs. Part two begins with a chapter exploring the methods and protocols of standardisation in cell and tissue engineering before moving on to highlight issues of quality control in cell and tissue engineering, standardised chemical analysis and testing of biomaterials and principles of good laboratory practice (GLP) for in vitro cell culture applications. Standardisation in cell and tissue engineering is a standard reference for leading research groups, government agencies, regulatory bodies, and researchers and technicians at all levels across the whole range of disciplines using cell culture within the pharmaceutical, biotechnology and biomedical industries. Introduces concepts and current practice in the field of cell and tissue engineering Highlights the importance of standardisation in cell and tissue engineering and suggests directions for further investigation Explores methods and protocols of standardisation in cell and tissue engineering and issues of quality control in cell and tissue engineering

This volume provides a state-of-art-report on the new methodologies in tissue engineering and developments in the biomaterials field based on the extracellular matrix-relevant discovery. Extracellular Matrix for Tissue Engineering and Biomaterials opens with an overview of the latest extracellular matrix research and in Part I, focuses on its biology and its role on cell behavior and cell fate relevant for the design of biomimetic surfaces. Part II details issues regarding the strategies currently applied in the research of biologically inspired materials and material systems for the replacement, repair and regeneration of tissues and organs. Part III presents the latest development methods applying knowledge from biology towards nanotechnology, to promote the restoration of the functionality of a living tissue. The book ranges from fundamental biology associated with tissue regeneration for the development of biomimetic approaches to controlling tissue formation, cell function, differentiation and angiogenesis using factors involved in normal tissue development and function. With the breadth and depth of the coverage of this topic, this book will serve as a valuable reference for anyone working in tissue engineering or biomaterials – from scientists, chemists and biologists through physicists, bioengineers and clinicians.

Tissue engineering research continues to captivate the interest of researchers and the general public alike. Popular media outlets like The New York Times, Time, and Wired continue to engage a wide audience and foster excitement for the field as regenerative medicine inches toward becoming a clinical reality. Putting the numerous advances in the fi