Colloidal Gold

Since its introduction in 1971, the development and application of colloidal gold as a marker in electron microscopy has been phenomenal. Colloidal gold has become the method of choice in immunocytochemistry and many areas of cell biology. This universal method is applicable to most microscopical systems including optical microscopy; scanning, transmission, and high voltage electron microscopy; photoelectron, photon, fluorescent darkfield, and epipolarization microscopy. Colloidal gold allows high and low resolution studies, enzyme and nucleic acid labeling, study of dynamic cellular processes, and virus detection. This book is among the first available to cover the principles and methodology of colloidal gold in microscopy. Methods are described step by step, to enable researchers to learn these complex procedures solely by reference to these books Problems and limitations of techniques are discussed Guides users to avoid problems and choose the correct procedures for specific applications Contributors are eminent authorities in their fields

Since its introduction in 1971, the development and application of colloidal gold as a marker in electron microscopy has been phenomenal. This state-of-the art, multi-volume treatise provides researchers, technicians, teachers, and students with the most comprehensive coverage of the principles and methodology of colloidal gold microscopy available today. This universal method is applicable to most microscopical systems including optical microscopy, scanning, transmission and high voltage electron microscopy, and photoelectron, photon, fluorescent darkfield and epipolarization microscopy. Colloidal gold allows high and low resolution studies, enzyme and nucleic acid labeling, study of dynamic cellular processes, and virus detection. Principles, methods, and applications of colloidal gold methodology in cytochemistry and immunochemistry Methods for preparing colloidal gold particles of different sizes Protein A-gold, protein G-gold, and lectin-gold techniques The use of resins and thin cryosections Multiple labeling

This book covers the synthesis and applications of colloidal gold nanorods including their properties, approaches for various chemical synthesis, and different gold nanorod-based nanocomposites with their properties and application potentials. Furthermore, it covers the surface chemistry and functionalization of gold nanorods for numerous biomedical applications. Various applications of gold nanorods including optical probes, dark filed contrast agents, photothermal therapy agents, and plasmonic photocatalyst are covered, along with the toxicological aspects. Features: Covers all aspects of gold nanorods along with selected protocols Focuses on synthetic chemistry, optical property, and functionalization approach of colloidal gold nanorods Describes standard synthetic methods and advantages of gold nanorods in biomedical applications Includes authentic and reproducible experimental procedures Discusses applications like redox catalysts, catalyst promoters, delivery carriers, solar cell materials, and so forth This book aims at graduate students and researchers interested in nanotechnology and gold nanoparticles.

Due to the simplicity, relative accuracy, fast result reporting, and user-friendliness of lateral flow immunoassay, its use has undergone tremendous growth in the diagnostic industry in the last few years. Such technology has been utilized widely and includes pregnancy and woman's health determination, cardiac and emergency conditions monitoring and testing, infectious disease including Flu screening, cancer marker screening, and drugs abuse testing. This book covers the scope of utilization, the principle of the technology, the patent concerns, information on the development and production of the test device and specific applications will be of interest to the diagnostic industry and the general scientific community.

Since its introduction in 1971, the development and application of colloidal gold as a marker in electron microscopy has been phenomenal. This state-of-the art, multi-volume treatise provides researchers, technicians, teachers, and students with the most comprehensive coverage of the principles and methodology of colloidal gold microscopy available today. Colloidal gold allows high and low resolution studies, enzyme and nucleic acid labeling, study of dynamic cellular processes, and virus detection. This third volume completes Hayat's coverage of the principles and methodology of colloidal gold in microscopy. The three-volume set should become the standard reference in the field. Among the first books to cover principles and methodology of colloidal gold in microscopy Describes methods step by step to enable researchers to learn these complex procedures solely by reference to the book Discusses problems and limitations of techniques Guides users to avoid problems and choose the correct procedures for specific applications Contributors are eminent authorities in their fields Topics covered in Volume 3 include: Production of monoclonal and polyclonal antibodies, Lowicryl, polar, and apolar resins Preserving and localizing antigenicity; sectioning; high-resolution morphological localization Diaminobenzidine-colloidal gold; microinjection of colloidal gold; double labeling with backscattered electrons; microwave irradiation Applications to biomedical studies Colloidal gold in conjunction with video-enhanced microscopy

Electron microscopy is frequently portrayed as a discipline that stands alone, separated from molecular biology, light microscopy, physiology, and biochemistry, among other disciplines. It is also presented as a technically demanding discipline operating largely in the sphere of "black boxes" and governed by many absolute laws of procedure. At the introductory level, this portrayal does the discipline and the student a disservice. The instrumentation we use is complex, but ultimately understandable and, more importantly, repairable. The procedures we employ for preparing tissues and cells are not totally understood, but enough information is available to allow investigators to make reasonable choices concerning the best techniques to apply to their parti cular problems. There are countless specialized techniques in the field of electron and light microscopy that require the acquisition of specialized knowledge, particularly for interpretation of results (electron tomography and energy dispersive spectroscopy immediately come to mind), but most laboratories possessing the equipment to effect these approaches have specialists to help the casual user. The advent of computer operated electron microscopes has also broadened access to these instruments, allowing users with little technical knowledge about electron microscope design to quickly become operators. This has been a welcome advance, because earlier instru ments required a level of knowledge about electron optics and vacuum systems to produce optimal photographs and to avoid "crashing" the instruments that typically made it difficult for beginners.